Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   BC375_RS10865 Genome accession   NZ_CP016610
Coordinates   2244354..2245217 (-) Length   287 a.a.
NCBI ID   WP_010894958.1    Uniprot ID   Q9PAI1
Organism   Xylella fastidiosa strain Salento-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2239354..2250217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BC375_RS10845 (BC375_10160) rimK 2239895..2240759 (+) 865 Protein_2030 30S ribosomal protein S6--L-glutamate ligase -
  BC375_RS10850 (BC375_10165) - 2241445..2242122 (+) 678 WP_010894955.1 response regulator transcription factor -
  BC375_RS10855 (BC375_10170) - 2242115..2243452 (+) 1338 WP_023908103.1 HAMP domain-containing sensor histidine kinase -
  BC375_RS10860 (BC375_10175) coaE 2243719..2244342 (-) 624 WP_046418843.1 dephospho-CoA kinase -
  BC375_RS10865 (BC375_10180) pilD 2244354..2245217 (-) 864 WP_010894958.1 A24 family peptidase Machinery gene
  BC375_RS10870 (BC375_10185) pilC 2245224..2246426 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  BC375_RS10875 (BC375_10190) pilA/pilAI 2246875..2247321 (+) 447 WP_010894960.1 pilin Machinery gene
  BC375_RS10880 (BC375_10195) - 2247582..2247785 (-) 204 WP_230950314.1 hypothetical protein -
  BC375_RS10885 (BC375_10200) pilA 2248617..2249054 (+) 438 WP_046418469.1 pilin Machinery gene

Sequence

Protein


Download         Length: 287 a.a.        Molecular weight: 31776.90 Da        Isoelectric Point: 8.6709

>NTDB_id=160758 BC375_RS10865 WP_010894958.1 2244354..2245217(-) (pilD) [Xylella fastidiosa strain Salento-2]
MAFLDQHPGLGYPLASGLGLLLGSFLNVVILRLPRRLEWQWKRDAREILEMPDIYDPPPPGIVVEPSHDPVTGDRLKWWE
NIPVLSWLLLRGRSRYSGKSISLQYPLVELLTCLLVLASVWRFGFGWHGFGAIVFSCFLVALAGIDLRTRLLPDQLTLML
MWLGLIGSTDNLFMAAKPALLGAVVGYLSLWIVAALFKLIFNKEGMGHGDFKMLAALGAWCGLKGILPIILISSLAGVVL
GSVWLVVRGHNRTTMIPFGPYLAVGGWIVFFFGDQIIHAYLELLSIG

Nucleotide


Download         Length: 864 bp        

>NTDB_id=160758 BC375_RS10865 WP_010894958.1 2244354..2245217(-) (pilD) [Xylella fastidiosa strain Salento-2]
ATGGCATTTCTTGACCAGCACCCCGGTCTCGGCTATCCCCTTGCGTCCGGTTTGGGGTTGTTGCTTGGTAGTTTTCTCAA
TGTGGTGATCCTGCGTTTGCCGCGCCGTTTGGAATGGCAATGGAAGCGCGATGCGCGCGAAATCCTGGAGATGCCGGACA
TTTATGATCCGCCACCACCGGGAATTGTGGTCGAGCCTTCCCACGATCCAGTGACTGGTGATCGGTTGAAGTGGTGGGAA
AACATTCCGGTGCTTAGCTGGTTGTTGCTACGTGGACGTTCGCGTTACAGCGGCAAGTCAATCTCGTTGCAGTATCCGTT
GGTCGAATTGCTGACTTGCCTGCTGGTGCTGGCCAGTGTTTGGCGTTTTGGATTCGGTTGGCATGGTTTTGGGGCAATTG
TGTTCAGTTGTTTCCTGGTTGCGTTGGCTGGGATTGATTTGCGTACTCGGCTCTTACCTGACCAGTTAACTTTAATGCTC
ATGTGGCTGGGTTTGATTGGATCGACGGATAACTTGTTTATGGCAGCCAAACCTGCGTTATTGGGGGCTGTAGTTGGTTA
TTTATCTCTGTGGATTGTGGCGGCGTTGTTCAAACTGATCTTTAACAAGGAAGGGATGGGCCATGGTGACTTCAAAATGC
TGGCCGCGCTTGGGGCATGGTGTGGGTTGAAGGGTATTCTCCCGATCATTTTGATTTCATCACTGGCTGGAGTGGTATTG
GGCTCCGTTTGGCTTGTTGTGCGTGGTCATAACCGTACAACGATGATTCCGTTTGGACCTTATCTGGCAGTTGGTGGATG
GATCGTGTTCTTTTTCGGCGATCAGATCATCCATGCTTATCTGGAGCTATTAAGTATCGGGTGA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(133-241)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9PAI1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

42.403

98.606

0.418
  pilD Vibrio campbellii strain DS40M4

41.754

99.303

0.415
  pilD Acinetobacter nosocomialis M2

42.049

98.606

0.415
  pilD Acinetobacter baumannii D1279779

42.049

98.606

0.415
  pilD Neisseria gonorrhoeae MS11

44.03

93.38

0.411