Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   RN80_RS01105 Genome accession   NZ_CP012646
Coordinates   195161..196102 (-) Length   313 a.a.
NCBI ID   WP_060627217.1    Uniprot ID   -
Organism   Streptococcus mitis strain KCOM 1350 (= ChDC B183)     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 190161..201102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RN80_RS01065 (RN80_01075) comYH 190761..191714 (-) 954 WP_049515760.1 class I SAM-dependent methyltransferase Machinery gene
  RN80_RS01070 (RN80_01080) - 191775..192362 (-) 588 WP_060627211.1 class I SAM-dependent methyltransferase -
  RN80_RS01075 (RN80_01085) comGG/cglG 192395..192808 (-) 414 WP_060627212.1 competence type IV pilus minor pilin ComGG Machinery gene
  RN80_RS01080 (RN80_01090) comGF/cglF 192786..193247 (-) 462 WP_060627213.1 competence type IV pilus minor pilin ComGF Machinery gene
  RN80_RS01085 (RN80_01095) comGE/cglE 193210..193512 (-) 303 WP_253275437.1 competence type IV pilus minor pilin ComGE Machinery gene
  RN80_RS01090 (RN80_01100) comGD/cglD 193475..193909 (-) 435 WP_060627215.1 competence type IV pilus minor pilin ComGD Machinery gene
  RN80_RS01095 (RN80_01105) comGC/cglC 193872..194195 (-) 324 WP_060627216.1 competence type IV pilus major pilin ComGC Machinery gene
  RN80_RS01100 (RN80_01110) comGB/cglB 194197..195213 (-) 1017 WP_080998467.1 competence type IV pilus assembly protein ComGB Machinery gene
  RN80_RS01105 (RN80_01115) comGA/cglA/cilD 195161..196102 (-) 942 WP_060627217.1 competence type IV pilus ATPase ComGA Machinery gene
  RN80_RS01110 (RN80_01120) - 196179..196544 (-) 366 WP_000286405.1 DUF1033 family protein -
  RN80_RS01115 (RN80_01125) - 196694..197752 (-) 1059 WP_060627218.1 zinc-dependent alcohol dehydrogenase family protein -
  RN80_RS01120 (RN80_01130) nagA 197915..199066 (-) 1152 WP_001134549.1 N-acetylglucosamine-6-phosphate deacetylase -
  RN80_RS01125 (RN80_01135) - 199218..201035 (-) 1818 WP_060627219.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35621.60 Da        Isoelectric Point: 6.0086

>NTDB_id=155982 RN80_RS01105 WP_060627217.1 195161..196102(-) (comGA/cglA/cilD) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGCYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQELHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDRETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQSTSWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=155982 RN80_RS01105 WP_060627217.1 195161..196102(-) (comGA/cglA/cilD) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCCCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATCGGTTGTTATGATTTTGAAAAATTTGCGGCAG
TCATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTAGGAGAAAAGCGACGTAGTCAACTTGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCATCTCTACGTCTATCTACTGTAGGCGATTATCGAGGACATGAGAGTTTAGTTATTCGTTTGTT
GCATGATGAGGAGCAGGAGTTGCATTTTTGGTTTCAGGATATTGAAGAACTGGGTAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGCCCAGTCGGCAGTGGCAAGACGACCCTGATGCACGAATTGGCCAAGTCTCTCTTTAAGGGGCAACAA
GTTATGTCTATTGAAGATCCAGTCGAAATAAAGCAGGACGACATGCTCCAATTGCAGTTGAACGAAGCAATCGGGTTGAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGAGACAGAGAGACGG
CGCGTGCAGTAGTTAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCATGCCAAGAGTATTCGAGGTGTTTAT
GAGCGCCTGCTGGAGTTGGGTGTGAGTGAGGAGGAATTAGCAGTCGTTCTGCAAGGAGTCTGCTACCAGAGATTAATTGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAAGAACACCAGTCAACTAGCTGGAATGAACAGATTGACCAGC
TTCTTAAAGATGGACATATCACAAGCCTTCAAGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

96.166

100

0.962
  comGA/cglA/cilD Streptococcus pneumoniae D39

96.166

100

0.962
  comGA/cglA/cilD Streptococcus pneumoniae R6

96.166

100

0.962
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

96.166

100

0.962
  comYA Streptococcus gordonii str. Challis substr. CH1

78.71

99.042

0.78
  comYA Streptococcus mutans UA159

65.273

99.361

0.649
  comYA Streptococcus mutans UA140

65.273

99.361

0.649
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

63.226

99.042

0.626
  comGA Lactococcus lactis subsp. cremoris KW2

55.449

99.681

0.553
  comGA Latilactobacillus sakei subsp. sakei 23K

43.019

84.665

0.364

Multiple sequence alignment