Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   RN80_RS01100 Genome accession   NZ_CP012646
Coordinates   194197..195213 (-) Length   338 a.a.
NCBI ID   WP_080998467.1    Uniprot ID   -
Organism   Streptococcus mitis strain KCOM 1350 (= ChDC B183)     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 189197..200213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RN80_RS01060 (RN80_01070) - 189521..190711 (-) 1191 WP_060627210.1 acetate kinase -
  RN80_RS01065 (RN80_01075) comYH 190761..191714 (-) 954 WP_049515760.1 class I SAM-dependent methyltransferase Machinery gene
  RN80_RS01070 (RN80_01080) - 191775..192362 (-) 588 WP_060627211.1 class I SAM-dependent methyltransferase -
  RN80_RS01075 (RN80_01085) comGG/cglG 192395..192808 (-) 414 WP_060627212.1 competence type IV pilus minor pilin ComGG Machinery gene
  RN80_RS01080 (RN80_01090) comGF/cglF 192786..193247 (-) 462 WP_060627213.1 competence type IV pilus minor pilin ComGF Machinery gene
  RN80_RS01085 (RN80_01095) comGE/cglE 193210..193512 (-) 303 WP_253275437.1 competence type IV pilus minor pilin ComGE Machinery gene
  RN80_RS01090 (RN80_01100) comGD/cglD 193475..193909 (-) 435 WP_060627215.1 competence type IV pilus minor pilin ComGD Machinery gene
  RN80_RS01095 (RN80_01105) comGC/cglC 193872..194195 (-) 324 WP_060627216.1 competence type IV pilus major pilin ComGC Machinery gene
  RN80_RS01100 (RN80_01110) comGB/cglB 194197..195213 (-) 1017 WP_080998467.1 competence type IV pilus assembly protein ComGB Machinery gene
  RN80_RS01105 (RN80_01115) comGA/cglA/cilD 195161..196102 (-) 942 WP_060627217.1 competence type IV pilus ATPase ComGA Machinery gene
  RN80_RS01110 (RN80_01120) - 196179..196544 (-) 366 WP_000286405.1 DUF1033 family protein -
  RN80_RS01115 (RN80_01125) - 196694..197752 (-) 1059 WP_060627218.1 zinc-dependent alcohol dehydrogenase family protein -
  RN80_RS01120 (RN80_01130) nagA 197915..199066 (-) 1152 WP_001134549.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38358.27 Da        Isoelectric Point: 9.3061

>NTDB_id=155981 RN80_RS01100 WP_080998467.1 194197..195213(-) (comGB/cglB) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
MDISQAFKLKRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRTGLSQGKSFSEMMESLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGFVSVGILLALTFYKRSSKMRAFSILARLPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQIMQEQGSQLFKEI
GQDLAQSLQNGCEFSQTIATYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=155981 RN80_RS01100 WP_080998467.1 194197..195213(-) (comGB/cglB) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
ATGGACATATCACAAGCCTTCAAGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTGTTTAA
CAATCTCTTTTCCAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGCACGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATTTTCAAGTGCCATT
GTCACTCAGTTATCCCTAGCGGAAGTCCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
AGCCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGATTTCTTCTCTTAATTATGCTGG
GACTACGTAATTACCTGCTACCACAACTGGATAGTAGTAATATTGCCACCCAAATCATTGGAAATCTCCCACAAATCTTT
CTAGGAATGGTAGGCTTTGTTTCAGTAGGTATCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGCGCGCCTT
TTCTATCTTAGCACGCCTTCCCTTTCTTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTATGCGCGTGAATGGGGGA
ATATGATTTCGCAGGGGATGGAGCTGACGCAGATTTTTCAGATCATGCAGGAACAAGGTTCTCAACTCTTTAAAGAAATC
GGTCAAGACCTAGCTCAATCCCTACAAAATGGCTGCGAATTTTCTCAGACGATAGCGACCTATCCTTTCTTTAGGAAAGA
GTTGAGTCTCATTATCGAGTATGGGGAAGTCAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAACTTAGTACAGCCACTGGTCTTTATCTTTGTGGCTCTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

96.45

100

0.965
  comGB/cglB Streptococcus mitis NCTC 12261

96.154

100

0.962
  comGB/cglB Streptococcus pneumoniae Rx1

94.97

100

0.95
  comGB/cglB Streptococcus pneumoniae D39

94.97

100

0.95
  comGB/cglB Streptococcus pneumoniae R6

94.97

100

0.95
  comGB/cglB Streptococcus pneumoniae TIGR4

94.97

100

0.95
  comYB Streptococcus gordonii str. Challis substr. CH1

72.619

99.408

0.722
  comYB Streptococcus mutans UA140

58.934

94.379

0.556
  comYB Streptococcus mutans UA159

58.934

94.379

0.556
  comGB Lactococcus lactis subsp. cremoris KW2

50.299

98.817

0.497

Multiple sequence alignment