Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LG3211_RS19060 Genome accession   NZ_CP011131
Coordinates   4699525..4700640 (+) Length   371 a.a.
NCBI ID   WP_057944209.1    Uniprot ID   -
Organism   Lysobacter gummosus strain 3.2.11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4694525..4705640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG3211_RS19040 (LG3211_4191) - 4695688..4697058 (-) 1371 WP_057944207.1 pyridoxal-phosphate dependent enzyme -
  LG3211_RS19045 (LG3211_4193) - 4697522..4697737 (-) 216 WP_057945597.1 YdcH family protein -
  LG3211_RS19050 (LG3211_4194) - 4698411..4699049 (-) 639 WP_057944208.1 hypothetical protein -
  LG3211_RS19055 (LG3211_4195) - 4699046..4699426 (-) 381 WP_083512669.1 DUF4398 domain-containing protein -
  LG3211_RS19060 (LG3211_4196) pilU 4699525..4700640 (+) 1116 WP_057944209.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG3211_RS19065 (LG3211_4197) maiA 4700819..4701481 (-) 663 WP_057944210.1 maleylacetoacetate isomerase -
  LG3211_RS19070 (LG3211_4198) - 4701670..4702656 (-) 987 WP_057944211.1 fumarylacetoacetate hydrolase family protein -
  LG3211_RS19075 (LG3211_4199) - 4703153..4705120 (+) 1968 WP_187313053.1 peptidoglycan DD-metalloendopeptidase family protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41050.44 Da        Isoelectric Point: 6.1912

>NTDB_id=142640 LG3211_RS19060 WP_057944209.1 4699525..4700640(+) (pilU) [Lysobacter gummosus strain 3.2.11]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGRLYPLGNTGLPPGMVKKIAYSLMDEGQVPLFERDLELNMALALPDAGR
FRINVFKQRGEVGMVIRAIRSIIPSIEELQLPQVLKNIIMAPRGLVLIVGSTGSGKSTTLASMIDHRNSSTAGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFPEAAHKNVLMNLALNLKAVVSQRLVIGVDGRRLPAAEILINTPHVRDLMRRGQVHEIKQAMEESLEEGMESFDQCL
FRLYKEGKIEMEEALKAADSRDGLALKFRLSEGGTGEHDPYADMYDASAAH

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=142640 LG3211_RS19060 WP_057944209.1 4699525..4700640(+) (pilU) [Lysobacter gummosus strain 3.2.11]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTCTTGACGACCGGCGCGCCGGTGTA
CATCAAGGTCGAAGGCCGTTTGTACCCGCTCGGCAATACGGGCCTGCCTCCGGGCATGGTCAAGAAGATCGCGTATTCGC
TGATGGACGAGGGCCAGGTGCCGCTGTTCGAGCGTGACCTCGAACTGAACATGGCCCTTGCCCTGCCCGACGCCGGCCGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTCATCCGCGCCATCCGCAGCATCATTCCGAGCATCGA
AGAGCTGCAGCTGCCGCAGGTGCTCAAGAACATCATCATGGCCCCGCGCGGGCTGGTGCTGATCGTGGGCTCCACCGGTT
CGGGCAAATCGACCACGCTGGCCTCGATGATCGACCATCGCAACTCCAGCACGGCCGGTCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACGCACGCCTTCCACAA
CGCGCTCAAGAACGCGATGCGCGAAGCGCCCGACGTGATCCTGATCGGCGAAATCCTGGACGCGACCACGATGGAAGCGG
CGATCGCCTTCGCCGAAACCGGCCATCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCCTGGAGCGCATC
CTCAACTTCTTCCCGGAAGCCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTCAAGGCCGTGGTCTCGCAGCG
CCTGGTGATCGGCGTGGACGGGCGTCGCCTGCCGGCGGCCGAGATCCTGATCAACACCCCGCACGTGCGCGATCTGATGC
GTCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAAGAGTCGCTGGAAGAAGGCATGGAGTCGTTCGACCAGTGCCTG
TTCCGCCTGTACAAGGAAGGCAAGATCGAGATGGAGGAGGCGCTCAAGGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCGGCACCGGCGAGCACGATCCGTATGCGGACATGTACGACGCGTCCGCGGCGCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.975

96.765

0.493
  pilU Acinetobacter baylyi ADP1

48.87

95.418

0.466
  pilU Vibrio cholerae strain A1552

48.305

95.418

0.461
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.608

91.375

0.426
  pilT Pseudomonas aeruginosa PAK

41.42

91.105

0.377
  pilT Acinetobacter baylyi ADP1

40.588

91.644

0.372
  pilT Acinetobacter baumannii strain A118

40.828

91.105

0.372
  pilT Acinetobacter nosocomialis M2

40.828

91.105

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.828

91.105

0.372
  pilT Acinetobacter baumannii D1279779

40.828

91.105

0.372
  pilT Legionella pneumophila strain Lp02

40.533

91.105

0.369
  pilT Legionella pneumophila strain ERS1305867

40.533

91.105

0.369
  pilT Neisseria gonorrhoeae MS11

39.193

93.531

0.367
  pilT Neisseria meningitidis 8013

39.193

93.531

0.367

Multiple sequence alignment