Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LA76x_RS04930 Genome accession   NZ_CP011129
Coordinates   1229094..1230212 (-) Length   372 a.a.
NCBI ID   WP_057916856.1    Uniprot ID   -
Organism   Lysobacter antibioticus strain 76     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1224094..1235212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA76x_RS04915 (LA76x_1061) - 1224903..1226873 (-) 1971 WP_057916853.1 M23 family metallopeptidase -
  LA76x_RS04920 (LA76x_1062) - 1227236..1228222 (+) 987 WP_057916854.1 fumarylacetoacetate hydrolase family protein -
  LA76x_RS04925 (LA76x_1063) maiA 1228320..1228982 (+) 663 WP_057916855.1 maleylacetoacetate isomerase -
  LA76x_RS04930 (LA76x_1064) pilU 1229094..1230212 (-) 1119 WP_057916856.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LA76x_RS04935 (LA76x_1065) - 1230309..1230686 (+) 378 WP_082638455.1 DUF4398 domain-containing protein -
  LA76x_RS04940 (LA76x_1066) - 1230683..1231312 (+) 630 WP_057916857.1 hypothetical protein -
  LA76x_RS26040 - 1231712..1231861 (+) 150 WP_169795634.1 hypothetical protein -
  LA76x_RS04945 (LA76x_1068) - 1231873..1232088 (+) 216 WP_031371506.1 YdcH family protein -
  LA76x_RS04950 (LA76x_1069) - 1232476..1233846 (+) 1371 WP_057916858.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41248.67 Da        Isoelectric Point: 6.1912

>NTDB_id=142591 LA76x_RS04930 WP_057916856.1 1229094..1230212(-) (pilU) [Lysobacter antibioticus strain 76]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGRLYPLGNTGLPPGMVKKIAYSLMDEGQVPLFERDLELNMALALPDAGR
FRINVFKQRGEVGMVIRAIRSIIPSIEELQLPQVLKQIIMAPRGLVLIVGSTGSGKSTTLASMIDHRNSSTAGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFPEAAHKNVLMNLALNLKAVVSQRLVIGLDGRRLPAAEILINTPHVRDLMRRGQVHEIKQAMEESLEEGMESFDQCL
FRLYKEGRIEMEEALKAADSRDGLALKFRLSEGGTGEHDPYADMYDATAAQH

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=142591 LA76x_RS04930 WP_057916856.1 1229094..1230212(-) (pilU) [Lysobacter antibioticus strain 76]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAAAAAAACGCGTCGGATATGTTCCTGACGACTGGCGCGCCGGTGTA
CATCAAGGTCGAGGGTCGACTGTACCCGCTCGGCAATACCGGTCTGCCCCCGGGCATGGTCAAGAAGATCGCCTATTCGT
TGATGGACGAGGGCCAGGTGCCGCTGTTCGAGCGTGACCTGGAGTTGAACATGGCGCTAGCCCTGCCCGACGCCGGCCGT
TTCCGCATCAACGTCTTCAAGCAGCGCGGCGAAGTCGGCATGGTCATCCGCGCCATCCGCAGCATCATTCCGAGCATCGA
AGAGCTGCAACTGCCGCAGGTGCTCAAGCAGATCATCATGGCGCCGCGCGGCCTGGTTCTGATCGTCGGTTCGACCGGCT
CGGGCAAGTCGACCACGCTGGCCTCGATGATCGACCACCGCAACAGCAGTACCGCCGGTCACATCCTCACCATCGAGGAT
CCGATCGAGTATCTGCACAAGCACAAGAAGTCGATCGTGAACCAGCGCGAGGTCGGCCTGGATACCCACGCCTTCCACAA
CGCGCTCAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTCGACGCCACGACCATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCGACGCTGCACTCGAACAACGCCGACCAGACCCTGGAGCGCATC
CTCAACTTCTTCCCCGAGGCCGCGCACAAGAACGTGTTGATGAACCTCGCCCTGAACCTCAAGGCGGTGGTCTCGCAACG
CCTGGTGATCGGCCTCGACGGGCGCCGCCTGCCGGCCGCGGAAATCCTCATCAACACCCCGCACGTGCGCGATCTGATGC
GCCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAAGAGTCGCTCGAAGAAGGCATGGAGTCGTTCGACCAGTGCCTG
TTCCGCCTCTACAAAGAGGGCCGGATCGAGATGGAGGAAGCGCTCAAGGCGGCCGACTCGCGCGACGGTCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGGCACCGGCGAGCACGATCCCTACGCGGACATGTACGACGCGACTGCCGCGCAGCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.975

96.505

0.492
  pilU Acinetobacter baylyi ADP1

48.87

95.161

0.465
  pilU Vibrio cholerae strain A1552

47.74

95.161

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.608

91.129

0.425
  pilT Pseudomonas aeruginosa PAK

41.42

90.86

0.376
  pilT Acinetobacter baylyi ADP1

40.588

91.398

0.371
  pilT Acinetobacter baumannii strain A118

40.828

90.86

0.371
  pilT Acinetobacter nosocomialis M2

40.828

90.86

0.371
  pilT Acinetobacter baumannii D1279779

40.828

90.86

0.371
  pilT Pseudomonas stutzeri DSM 10701

40.533

90.86

0.368
  pilT Legionella pneumophila strain Lp02

40.533

90.86

0.368
  pilT Legionella pneumophila strain ERS1305867

40.533

90.86

0.368
  pilT Neisseria gonorrhoeae MS11

39.193

93.28

0.366
  pilT Neisseria meningitidis 8013

39.193

93.28

0.366

Multiple sequence alignment