Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   AzCIB_RS19835 Genome accession   NZ_CP011072
Coordinates   4428143..4428571 (+) Length   142 a.a.
NCBI ID   WP_050418476.1    Uniprot ID   -
Organism   Azoarcus sp. CIB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4423143..4433571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AzCIB_RS19815 (AzCIB_3967) - 4426351..4426653 (+) 303 WP_050417475.1 hypothetical protein -
  AzCIB_RS19820 (AzCIB_3968) - 4426650..4427198 (+) 549 WP_050417476.1 hypothetical protein -
  AzCIB_RS19825 (AzCIB_3969) - 4427302..4427565 (-) 264 WP_050417477.1 type II toxin-antitoxin system RelE/ParE family toxin -
  AzCIB_RS19830 (AzCIB_3970) - 4427552..4427785 (-) 234 WP_050417478.1 ribbon-helix-helix protein, CopG family -
  AzCIB_RS19835 (AzCIB_3971) pilA2 4428143..4428571 (+) 429 WP_050418476.1 pilin Machinery gene
  AzCIB_RS19840 (AzCIB_3972) - 4428997..4430100 (+) 1104 WP_050417479.1 transposase -
  AzCIB_RS19845 (AzCIB_3973) - 4430200..4430685 (-) 486 WP_050417480.1 hypothetical protein -
  AzCIB_RS19850 (AzCIB_3974) - 4430682..4430981 (-) 300 WP_050417481.1 nucleotidyltransferase domain-containing protein -
  AzCIB_RS19855 (AzCIB_3975) pilR 4431075..4432466 (-) 1392 WP_050417482.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14358.55 Da        Isoelectric Point: 5.7581

>NTDB_id=142036 AzCIB_RS19835 WP_050418476.1 4428143..4428571(+) (pilA2) [Azoarcus sp. CIB]
MKKVQQGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVSEALVQAAPAKLAVAETAAALGGLANVTAANSGFAFSGTT
NVKSVDITDATGVITVVTQATGAAVDPAFTLTPTPGASTDDPLTWACAKTAGETKHLPATCR

Nucleotide


Download         Length: 429 bp        

>NTDB_id=142036 AzCIB_RS19835 WP_050418476.1 4428143..4428571(+) (pilA2) [Azoarcus sp. CIB]
ATGAAAAAGGTCCAGCAAGGCTTTACCCTGATCGAACTGATGATCGTTGTGGCGATTATCGGTATTTTGGCTGCTGTGGC
GCTGCCGGCGTATCAGGATTACACGATCCGGGCGCGTGTTTCGGAGGCTTTGGTCCAAGCCGCGCCGGCAAAACTCGCCG
TTGCCGAAACCGCGGCTGCGCTCGGTGGCCTCGCCAACGTCACGGCAGCCAATAGCGGCTTTGCGTTTAGCGGTACGACG
AACGTCAAATCGGTCGACATTACGGATGCAACGGGCGTCATCACTGTAGTCACTCAGGCTACAGGCGCAGCGGTCGATCC
GGCATTTACGCTGACCCCCACGCCCGGCGCGTCGACCGACGACCCGCTCACTTGGGCGTGCGCCAAAACGGCTGGCGAGA
CGAAGCACCTGCCTGCAACCTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

52.899

97.183

0.514
  pilA2 Legionella pneumophila str. Paris

52.899

97.183

0.514
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.945

100

0.493
  pilA Ralstonia pseudosolanacearum GMI1000

42.073

100

0.486
  pilE Neisseria gonorrhoeae MS11

41.718

100

0.479
  pilE Neisseria gonorrhoeae strain FA1090

40.881

100

0.458
  pilA/pilA1 Eikenella corrodens VA1

41.83

100

0.451
  comP Acinetobacter baylyi ADP1

42.857

100

0.444
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.781

100

0.43
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.426

99.296

0.401
  pilA Glaesserella parasuis strain SC1401

34.839

100

0.38
  pilA Acinetobacter baumannii strain A118

37.063

100

0.373

Multiple sequence alignment