Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N805_RS23855 Genome accession   NZ_CP010979
Coordinates   5390707..5391705 (+) Length   332 a.a.
NCBI ID   WP_019472089.1    Uniprot ID   A0AAU8S332
Organism   Pseudomonas putida S13.1.2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5385707..5396705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N805_RS23825 (N805_23830) - 5386184..5386618 (-) 435 WP_019472084.1 DUF4426 domain-containing protein -
  N805_RS23830 (N805_23835) metW 5386641..5387261 (-) 621 WP_003249106.1 methionine biosynthesis protein MetW -
  N805_RS23835 (N805_23840) metX 5387269..5388408 (-) 1140 WP_019472085.1 homoserine O-succinyltransferase MetX -
  N805_RS23840 (N805_23845) - 5388497..5389087 (-) 591 WP_019472086.1 YggT family protein -
  N805_RS23845 (N805_23850) proC 5389098..5389916 (-) 819 WP_019472087.1 pyrroline-5-carboxylate reductase -
  N805_RS23850 (N805_23855) - 5389955..5390641 (-) 687 WP_019472088.1 YggS family pyridoxal phosphate-dependent enzyme -
  N805_RS23855 (N805_23860) pilT 5390707..5391705 (+) 999 WP_019472089.1 type IV pilus twitching motility protein PilT Machinery gene
  N805_RS23860 (N805_23865) - 5391817..5392443 (-) 627 WP_019472090.1 C40 family peptidase -
  N805_RS23865 (N805_23870) - 5392610..5393026 (+) 417 WP_016489634.1 NINE protein -
  N805_RS23870 (N805_23875) - 5393093..5393791 (-) 699 WP_019472091.1 SPOR domain-containing protein -
  N805_RS23875 (N805_23880) argS 5393793..5395529 (-) 1737 WP_019472092.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36007.56 Da        Isoelectric Point: 6.3973

>NTDB_id=141083 N805_RS23855 WP_019472089.1 5390707..5391705(+) (pilT) [Pseudomonas putida S13.1.2]
MDVTDLLARAVNAGASDLHLAAGQIPMLRLEGDLQRMNFPALVPADLAEGMAPWLAEHQRRQWAEGDELDLALELPALGR
FRLNLFRQLNGPGVTFRLIPGRIATLDELDLGDVYQAVAQCTDGLILVGGPTGSGKSSTLATLIDQLNRDRALHIVTLED
PVEVIHSSQRSLINQREIGRHSGGFAQGLRSALRQDPDVIMIGELRDLESVRLALRAAETGHLVLATVHTRSAASSIDRL
VEVFAAEEKPLVRAMVAESLRLVVVQTLVRRVGGGRVAAREVLVATPAVRNLVREGRMAQLCSVMQAGGAEGMRTMEGAM
RELREKGLIKDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=141083 N805_RS23855 WP_019472089.1 5390707..5391705(+) (pilT) [Pseudomonas putida S13.1.2]
ATGGATGTGACCGACCTGTTGGCCCGGGCCGTAAATGCGGGGGCTTCGGACCTGCACCTGGCGGCGGGCCAGATCCCGAT
GCTGCGCCTGGAGGGTGACTTGCAGCGCATGAATTTCCCGGCGCTGGTGCCGGCAGACCTGGCCGAGGGCATGGCGCCCT
GGCTGGCTGAACACCAAAGGCGGCAGTGGGCCGAGGGCGATGAGCTGGACCTGGCGCTTGAGTTGCCGGCGCTTGGCCGC
TTTCGGCTGAACCTGTTTCGCCAACTGAACGGCCCTGGGGTTACCTTTAGGCTGATCCCGGGGCGGATCGCCACGCTGGA
TGAGCTCGACCTGGGGGATGTGTATCAAGCTGTTGCGCAATGCACTGATGGCCTGATCCTGGTAGGTGGGCCTACCGGCA
GTGGCAAGTCCAGCACCCTGGCGACGTTGATCGACCAGCTGAACCGCGACCGGGCGCTGCACATCGTCACCCTCGAAGAC
CCGGTCGAAGTTATCCACAGCAGCCAGCGCAGCCTGATCAACCAGCGTGAGATTGGCCGCCATAGCGGCGGCTTCGCCCA
GGGGCTGCGCAGTGCGCTGCGCCAGGACCCGGATGTGATCATGATCGGTGAGCTACGTGACCTGGAGAGCGTTCGCCTGG
CCTTGCGCGCAGCCGAGACCGGGCACCTGGTGCTGGCGACCGTGCATACGCGTTCGGCGGCCAGCAGCATTGACCGGCTG
GTGGAGGTGTTTGCTGCCGAGGAAAAGCCCTTGGTGCGGGCGATGGTGGCCGAGTCGCTGCGCCTTGTGGTGGTGCAGAC
GCTGGTCAGGCGTGTGGGCGGTGGGCGGGTGGCGGCGCGGGAAGTACTGGTGGCGACGCCGGCGGTGCGCAACCTGGTTC
GGGAGGGGCGAATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGTGGTGCAGAAGGGATGCGGACGATGGAGGGGGCAATG
CGGGAGCTGAGAGAGAAGGGGCTGATCAAAGACCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.064

99.096

0.506
  pilT Pseudomonas stutzeri DSM 10701

50.456

99.096

0.5
  pilT Acinetobacter baylyi ADP1

50.303

99.398

0.5
  pilT Acinetobacter nosocomialis M2

50.152

99.096

0.497
  pilT Acinetobacter baumannii D1279779

50.152

99.096

0.497
  pilT Acinetobacter baumannii strain A118

50.152

99.096

0.497
  pilT Vibrio cholerae strain A1552

48.632

99.096

0.482
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.632

99.096

0.482
  pilT Neisseria meningitidis 8013

48.182

99.398

0.479
  pilT Neisseria gonorrhoeae MS11

47.879

99.398

0.476
  pilT Legionella pneumophila strain Lp02

47.416

99.096

0.47
  pilT Legionella pneumophila strain ERS1305867

47.416

99.096

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.364

99.398

0.461
  pilU Pseudomonas stutzeri DSM 10701

36.97

99.398

0.367

Multiple sequence alignment