Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IX91_RS01735 Genome accession   NZ_CP009354
Coordinates   344873..345979 (+) Length   368 a.a.
NCBI ID   WP_004742513.1    Uniprot ID   F9SZF8
Organism   Vibrio tubiashii ATCC 19109     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 339873..350979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IX91_RS01700 (IX91_01740) - 340206..340811 (-) 606 WP_004742506.1 XTP/dITP diphosphatase -
  IX91_RS01705 (IX91_01745) - 340867..341298 (-) 432 WP_004742507.1 DUF4426 domain-containing protein -
  IX91_RS01710 (IX91_01750) yggU 341352..341642 (-) 291 WP_004742508.1 DUF167 family protein YggU -
  IX91_RS01715 (IX91_01755) - 341642..342199 (-) 558 WP_004742509.1 YggT family protein -
  IX91_RS01720 (IX91_01760) proC 342239..343057 (-) 819 WP_004742510.1 pyrroline-5-carboxylate reductase -
  IX91_RS01725 (IX91_01765) - 343083..343799 (-) 717 WP_004742511.1 YggS family pyridoxal phosphate-dependent enzyme -
  IX91_RS01730 (IX91_01770) pilT 343825..344862 (+) 1038 WP_004742512.1 type IV pilus twitching motility protein PilT Machinery gene
  IX91_RS01735 (IX91_01775) pilU 344873..345979 (+) 1107 WP_004742513.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IX91_RS25690 - 346068..346223 (-) 156 WP_004742514.1 FeoB-associated Cys-rich membrane protein -
  IX91_RS01740 (IX91_01785) feoB 346226..348727 (-) 2502 WP_004742515.1 ferrous iron transport protein B -
  IX91_RS01745 (IX91_01790) - 348751..348981 (-) 231 WP_004742516.1 FeoA family protein -
  IX91_RS01750 (IX91_01795) - 349201..349512 (+) 312 WP_004742517.1 hypothetical protein -
  IX91_RS01755 (IX91_01800) - 349538..350323 (+) 786 WP_004742518.1 DUF4198 domain-containing protein -
  IX91_RS01760 (IX91_01805) ruvX 350396..350821 (-) 426 WP_004742519.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41340.09 Da        Isoelectric Point: 6.1132

>NTDB_id=128844 IX91_RS01735 WP_004742513.1 344873..345979(+) (pilU) [Vibrio tubiashii ATCC 19109]
MDIDVFLQQMIDQNASDLYITVDAPILYRVDGELRAHGDKLSHAVVSSLLDGIMDHDRQHEFHSSKEANFAIVRHFGRFR
VSAFYQRELPGAVIRRIETKIPTFDELKLPDVLQDLSIAKRGLVLVVGATGSGKSTTMAAMTGFRNSQRTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVIGQQLIRDKNGQGRHGVFEVLLNSPRVSDLIRRGDLHELKATMAKSKEVGMQTFDQALY
QLVVEDKISEEDAMHSADSANDLRLMLKTQRGDVSSSGSLSGVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=128844 IX91_RS01735 WP_004742513.1 344873..345979(+) (pilU) [Vibrio tubiashii ATCC 19109]
ATGGATATTGATGTTTTTTTGCAACAGATGATCGACCAGAATGCGTCTGATCTTTACATTACAGTTGATGCGCCAATTCT
TTACCGTGTCGATGGAGAGTTGCGTGCACACGGGGACAAACTATCGCACGCTGTTGTGAGCTCTTTGTTGGATGGGATTA
TGGATCATGATCGCCAACACGAGTTTCATTCGAGCAAGGAAGCGAACTTTGCCATTGTGCGTCACTTTGGTCGATTCCGT
GTCTCTGCATTTTATCAGCGCGAATTGCCTGGGGCGGTGATTCGCCGAATTGAAACCAAGATTCCTACCTTTGATGAGCT
TAAGTTACCAGATGTTTTGCAAGACTTATCGATTGCGAAACGGGGTTTGGTGCTGGTGGTTGGAGCGACGGGATCGGGTA
AGTCGACGACCATGGCTGCAATGACAGGGTTTCGTAATAGCCAGCGGACAGGGCATATTTTAACTGTTGAAGACCCGATA
GAGTTTGTTCATGAGCATCAGCGTTGTATTGTTACGCAACGTGAAGTGGGGTTAGATACCGAAAGCTATGAAGTGGCGTT
GAAGAACTCACTGCGCCAAGCTCCAGATATGATCTTAATAGGTGAGATTCGTACACGTGAGACGATGGAATACGCAATGA
CCTTTGCTGAAACTGGGCATCTGTGTATGGCGACCCTTCATGCAAACAATGCTAACCAAGCGTTAGAGCGGATCCTTCAT
TTGGTGCCTAAGGAACAAAAAGAACAGTTCCTATTTGATTTATCGATGAACCTACGTGGTGTGATAGGTCAGCAACTTAT
TCGAGATAAAAATGGTCAAGGTCGGCACGGCGTGTTTGAAGTTTTACTCAACAGCCCACGTGTGTCGGATCTGATTCGCC
GCGGCGATCTGCATGAGCTTAAAGCGACCATGGCTAAATCGAAAGAGGTTGGCATGCAAACTTTTGATCAGGCGCTTTAT
CAACTAGTAGTGGAAGACAAGATCAGTGAAGAAGATGCCATGCACAGTGCAGATTCAGCTAATGATTTACGCCTAATGCT
TAAAACACAACGTGGTGATGTATCTAGCTCAGGATCACTCAGTGGCGTGAAGATTGATATGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F9SZF8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.337

100

0.823
  pilU Pseudomonas stutzeri DSM 10701

57.143

95.109

0.543
  pilU Acinetobacter baylyi ADP1

53.276

95.38

0.508
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.593

92.12

0.383
  pilT Legionella pneumophila strain Lp02

41.003

92.12

0.378
  pilT Legionella pneumophila strain ERS1305867

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

40.762

92.663

0.378
  pilT Acinetobacter nosocomialis M2

40.708

92.12

0.375
  pilT Acinetobacter baumannii D1279779

40.708

92.12

0.375
  pilT Acinetobacter baumannii strain A118

40.708

92.12

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.118

92.12

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.923

88.315

0.361
  pilT Vibrio cholerae strain A1552

40.923

88.315

0.361

Multiple sequence alignment