Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HL41_RS01830 Genome accession   NZ_CP008796
Coordinates   356917..358008 (+) Length   363 a.a.
NCBI ID   WP_038060022.1    Uniprot ID   A0A075WTF7
Organism   Thermodesulfobacterium commune DSM 2178     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 351917..363008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HL41_RS01810 (HL41_01875) - 352066..353568 (-) 1503 WP_038060024.1 UvrD-helicase domain-containing protein -
  HL41_RS01815 (HL41_01880) mnmA 353591..354607 (-) 1017 WP_038060023.1 tRNA 2-thiouridine(34) synthase MnmA -
  HL41_RS01820 - 354676..356418 (+) 1743 WP_051754430.1 YcaO-like family protein -
  HL41_RS01825 (HL41_01890) - 356435..356869 (+) 435 WP_000262566.1 NIL domain-containing protein -
  HL41_RS01830 (HL41_01895) pilT 356917..358008 (+) 1092 WP_038060022.1 type IV pilus twitching motility protein PilT Machinery gene
  HL41_RS01835 (HL41_01900) - 358016..359179 (+) 1164 WP_038060021.1 type IV pilus twitching motility protein PilT -
  HL41_RS01840 (HL41_01905) - 359189..359665 (+) 477 WP_038060020.1 hypothetical protein -
  HL41_RS01845 (HL41_01910) - 359665..360546 (+) 882 WP_051754431.1 UTP--glucose-1-phosphate uridylyltransferase -
  HL41_RS01850 (HL41_01915) priA 360569..362926 (+) 2358 WP_038060016.1 primosomal protein N' -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 41295.05 Da        Isoelectric Point: 6.5110

>NTDB_id=124267 HL41_RS01830 WP_038060022.1 356917..358008(+) (pilT) [Thermodesulfobacterium commune DSM 2178]
MAKLDAFFKLMVDTQASDLHLSAGNPPLLRIHGDLQRVKYKVLEDEELREMLYEIAPEEIIKKFEEQGEVDWGYEIPGLA
RFRVNYYRQRRGVAAAFRLIPNKIKTVEELGLPPILNRLALLPRGLVLVTGPTGSGKSTTLAAIIDYANRMRYDHIITIE
DPIEFVHEPKNCLINQREVGTHTKSFANALRAALREDPDIIMIGEMRDLETISLALEAALTGHLVFATLHTISAAQTVSR
IVDAFPPEERDQIRVSLSEALRAVISQTMFKRIDRPGRIVAMEIMIATPAIRNLIRENKIHQIPSMIQMGRKYGMMSLDD
CIMEYLNNKWISPEEAFIKAVDKSRFIPFVQNKEILDFTEMPG

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=124267 HL41_RS01830 WP_038060022.1 356917..358008(+) (pilT) [Thermodesulfobacterium commune DSM 2178]
ATGGCGAAGTTAGACGCTTTTTTTAAATTGATGGTTGACACCCAAGCTTCTGACCTGCATCTTTCTGCAGGTAATCCTCC
CCTTCTTAGGATCCACGGCGACCTTCAAAGGGTTAAATATAAAGTTTTAGAAGATGAAGAACTTAGAGAAATGCTCTATG
AAATAGCTCCTGAAGAAATCATCAAAAAGTTTGAAGAACAAGGGGAAGTAGACTGGGGTTATGAAATTCCTGGTTTAGCT
CGTTTTAGGGTGAACTATTATCGTCAAAGAAGAGGGGTTGCTGCAGCCTTTAGGCTTATTCCTAACAAGATAAAAACTGT
AGAAGAATTAGGTCTTCCTCCTATCCTTAATCGGTTAGCTCTTTTACCAAGAGGTTTAGTTTTGGTTACTGGTCCTACAG
GGTCAGGTAAATCTACAACCTTAGCTGCTATTATAGACTATGCCAACAGAATGCGTTATGACCATATTATTACCATAGAA
GACCCTATCGAGTTTGTGCATGAACCTAAGAATTGCTTAATAAACCAAAGAGAGGTGGGTACCCATACTAAAAGTTTTGC
TAATGCCCTTAGGGCTGCTCTGAGAGAAGACCCTGATATTATCATGATAGGTGAAATGAGAGATTTAGAAACCATTTCTT
TAGCTCTTGAAGCAGCTTTAACCGGACATTTAGTATTTGCTACCCTTCATACCATCAGCGCAGCTCAAACTGTGAGTAGA
ATTGTGGATGCTTTTCCTCCGGAGGAAAGAGACCAGATAAGGGTTTCTTTATCAGAGGCTTTAAGAGCAGTTATTTCTCA
AACGATGTTTAAAAGGATAGACAGACCTGGTAGAATTGTGGCTATGGAAATCATGATAGCTACTCCTGCTATCAGAAATC
TGATAAGAGAAAATAAGATCCATCAAATACCTTCGATGATTCAGATGGGTAGAAAATATGGGATGATGTCTCTTGATGAT
TGTATTATGGAATATCTTAACAACAAGTGGATAAGCCCTGAAGAAGCCTTTATTAAGGCAGTTGATAAAAGTAGGTTTAT
TCCTTTTGTCCAAAATAAAGAAATTTTAGACTTCACCGAAATGCCGGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-243)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A075WTF7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

58.462

89.532

0.523
  pilT Acinetobacter baylyi ADP1

57.057

91.736

0.523
  pilT Legionella pneumophila strain Lp02

54.913

95.317

0.523
  pilT Legionella pneumophila strain ERS1305867

54.913

95.317

0.523
  pilT Acinetobacter nosocomialis M2

55.357

92.562

0.512
  pilT Acinetobacter baumannii D1279779

55.357

92.562

0.512
  pilT Acinetobacter baumannii strain A118

55.357

92.562

0.512
  pilT Pseudomonas stutzeri DSM 10701

56.193

91.185

0.512
  pilT Neisseria meningitidis 8013

52.907

94.766

0.501
  pilT Neisseria gonorrhoeae MS11

52.616

94.766

0.499
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.672

90.634

0.468
  pilT Vibrio cholerae strain A1552

51.672

90.634

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.493

93.388

0.444
  pilU Acinetobacter baylyi ADP1

38.757

93.113

0.361

Multiple sequence alignment