Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   ACLV7A_RS01080 Genome accession   NZ_OZ217344
Coordinates   208569..209510 (+) Length   313 a.a.
NCBI ID   WP_411863363.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis E22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 203569..214510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7A_RS01060 (SMIE22_02050) - 203638..205455 (+) 1818 WP_411863362.1 acyltransferase family protein -
  ACLV7A_RS01065 (SMIE22_02060) nagA 205607..206758 (+) 1152 WP_218757951.1 N-acetylglucosamine-6-phosphate deacetylase -
  ACLV7A_RS01070 (SMIE22_02070) - 206921..207979 (+) 1059 WP_084886478.1 zinc-dependent alcohol dehydrogenase family protein -
  ACLV7A_RS01075 (SMIE22_02080) - 208129..208494 (+) 366 WP_045606653.1 DUF1033 family protein -
  ACLV7A_RS01080 (SMIE22_02090) comGA/cglA/cilD 208569..209510 (+) 942 WP_411863363.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLV7A_RS01085 (SMIE22_02100) comGB/cglB 209458..210474 (+) 1017 WP_411864201.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLV7A_RS01090 (SMIE22_02110) comGC/cglC 210476..210799 (+) 324 WP_000738649.1 competence type IV pilus major pilin ComGC Machinery gene
  ACLV7A_RS01095 (SMIE22_02120) comGD/cglD 210762..211196 (+) 435 WP_411863364.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLV7A_RS01100 (SMIE22_02130) comGE/cglE 211159..211461 (+) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACLV7A_RS01105 (SMIE22_02140) comGF/cglF 211424..211885 (+) 462 WP_000250514.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLV7A_RS01110 (SMIE22_02150) comGG/cglG 211863..212276 (+) 414 WP_411863365.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLV7A_RS01115 (SMIE22_02160) - 212309..212896 (+) 588 WP_411863366.1 class I SAM-dependent methyltransferase -
  ACLV7A_RS01120 (SMIE22_02170) comYH 212957..213910 (+) 954 WP_411863367.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35554.47 Da        Isoelectric Point: 5.8143

>NTDB_id=1170252 ACLV7A_RS01080 WP_411863363.1 208569..209510(+) (comGA/cglA/cilD) [Streptococcus mitis isolate S. mitis E22]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGCYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDDKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQNIDELGKQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNEQIDQLFKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1170252 ACLV7A_RS01080 WP_411863363.1 208569..209510(+) (comGA/cglA/cilD) [Streptococcus mitis isolate S. mitis E22]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCCCGGAAAAAAGGGGCGCAGGATATTTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATCGGTTGTTATGATTTTGAAAAGTTTGCGGCTG
TCATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTTGGAGAAAAGCGACGTAGTCAACTTGGTTCCTGTGATTATGCA
TATGACGATAAGATGGCGTCACTACGTTTATCTACCGTAGGCGATTATCGAGGTCATGAGAGTTTGGTGATCCGTTTGTT
GCATGATGAGGAGCAGGATTTGCATTTTTGGTTTCAGAATATTGACGAATTGGGCAAGCAGTATAGACAACGGGGGCTTT
ATCTTTTTGCTGGCCCAGTCGGCAGTGGCAAGACGACCCTGATGCACGAATTGGCCAAGTCTCTCTTTAAGGGGCAACAA
GTTATGTCTATTGAAGATCCAGTCGAAATCAAGCAGGACGACATGCTCCAGTTGCAGTTGAATGAGGCGATTGGGCTTAC
CTATGAAAATCTAATCAAACTTTCTTTGCGTCATCGTCCTGATCTCTTGATTATCGGAGAAATTCGGGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCATGCCAAGAGTATTCGAGGTGTTTAT
GAGCGCTTGCTGGAGTTGGGTGTGAGTGAGGAAGAATTAGCAGTCGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGAACAGATTGACCAGC
TTTTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

97.444

100

0.974
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae D39

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae R6

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

95.847

100

0.958
  comYA Streptococcus gordonii str. Challis substr. CH1

79.032

99.042

0.783
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.258

99.042

0.617
  comGA Lactococcus lactis subsp. cremoris KW2

55.449

99.681

0.553
  comGA Latilactobacillus sakei subsp. sakei 23K

42.647

86.901

0.371

Multiple sequence alignment