Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE/cglE   Type   Machinery gene
Locus tag   ACLV7A_RS01100 Genome accession   NZ_OZ217344
Coordinates   211159..211461 (+) Length   100 a.a.
NCBI ID   WP_000413380.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis E22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 206159..216461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7A_RS01070 (SMIE22_02070) - 206921..207979 (+) 1059 WP_084886478.1 zinc-dependent alcohol dehydrogenase family protein -
  ACLV7A_RS01075 (SMIE22_02080) - 208129..208494 (+) 366 WP_045606653.1 DUF1033 family protein -
  ACLV7A_RS01080 (SMIE22_02090) comGA/cglA/cilD 208569..209510 (+) 942 WP_411863363.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLV7A_RS01085 (SMIE22_02100) comGB/cglB 209458..210474 (+) 1017 WP_411864201.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLV7A_RS01090 (SMIE22_02110) comGC/cglC 210476..210799 (+) 324 WP_000738649.1 competence type IV pilus major pilin ComGC Machinery gene
  ACLV7A_RS01095 (SMIE22_02120) comGD/cglD 210762..211196 (+) 435 WP_411863364.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLV7A_RS01100 (SMIE22_02130) comGE/cglE 211159..211461 (+) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACLV7A_RS01105 (SMIE22_02140) comGF/cglF 211424..211885 (+) 462 WP_000250514.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLV7A_RS01110 (SMIE22_02150) comGG/cglG 211863..212276 (+) 414 WP_411863365.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLV7A_RS01115 (SMIE22_02160) - 212309..212896 (+) 588 WP_411863366.1 class I SAM-dependent methyltransferase -
  ACLV7A_RS01120 (SMIE22_02170) comYH 212957..213910 (+) 954 WP_411863367.1 class I SAM-dependent methyltransferase Machinery gene
  ACLV7A_RS01125 (SMIE22_02180) - 213961..215151 (+) 1191 WP_000167777.1 acetate kinase -
  ACLV7A_RS01130 (SMIE22_02190) rnpA 215299..215670 (+) 372 WP_000739245.1 ribonuclease P protein component -

Sequence

Protein


Download         Length: 100 a.a.        Molecular weight: 11135.11 Da        Isoelectric Point: 9.7273

>NTDB_id=1170256 ACLV7A_RS01100 WP_000413380.1 211159..211461(+) (comGE/cglE) [Streptococcus mitis isolate S. mitis E22]
MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVAKMALQTGQNQVNINGVEIQVFS
SEKGLEVYHGSEQLLAIKEP

Nucleotide


Download         Length: 303 bp        

>NTDB_id=1170256 ACLV7A_RS01100 WP_000413380.1 211159..211461(+) (comGE/cglE) [Streptococcus mitis isolate S. mitis E22]
ATGGAAAAATTAAACGCATTAAGGAAACAAAAAATTAGGGCAGTGATTTTACTGGAAGCAGTAGTGGCTCTAGCTATCTT
TGCTAGTATTGCTACCCTTCTTTTGGGACAAATTCAAAAGAATAGGCAAGAAGAAGCAAAAATCTTGCAGAAAGAAGAAG
TCTTGCGTGTAGCTAAGATGGCACTGCAGACAGGTCAAAATCAGGTAAACATAAACGGAGTGGAGATTCAGGTGTTTTCT
AGTGAAAAGGGATTGGAGGTTTATCATGGTTCAGAACAGTTGTTGGCTATCAAAGAGCCATAA

Domains

Predicted by InterproScan.

Type II secretory pathway pseudopilin (ComGE)

(16-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE/cglE Streptococcus mitis NCTC 12261

99

100

0.99
  comGE/cglE Streptococcus pneumoniae Rx1

99

100

0.99
  comGE/cglE Streptococcus pneumoniae D39

99

100

0.99
  comGE/cglE Streptococcus pneumoniae R6

99

100

0.99
  comGE/cglE Streptococcus pneumoniae TIGR4

99

100

0.99
  comGE/cglE Streptococcus mitis SK321

98

100

0.98
  comYE Streptococcus mutans UA140

43.333

90

0.39
  comYE Streptococcus mutans UA159

43.333

90

0.39

Multiple sequence alignment