Detailed information NTG1170257

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: ACLV7A_RS01105
• Genome accession: NZ_OZ217344
• Coordinates: 211424..211885 (+)
• Length: 153 a.a.
• NCBI ID: WP_000250514.1
• Uniprot ID: E9FQG6
• Organism: Streptococcus mitis isolate S. mitis E22
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 206424..216885

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLV7A_RS01070 (SMIE22_02070)	-	206921..207979 (+)	1059	WP_084886478.1	zinc-dependent alcohol dehydrogenase family protein	-
ACLV7A_RS01075 (SMIE22_02080)	-	208129..208494 (+)	366	WP_045606653.1	DUF1033 family protein	-
ACLV7A_RS01080 (SMIE22_02090)	comGA/cglA/cilD	208569..209510 (+)	942	WP_411863363.1	competence type IV pilus ATPase ComGA	Machinery gene
ACLV7A_RS01085 (SMIE22_02100)	comGB/cglB	209458..210474 (+)	1017	WP_411864201.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACLV7A_RS01090 (SMIE22_02110)	comGC/cglC	210476..210799 (+)	324	WP_000738649.1	competence type IV pilus major pilin ComGC	Machinery gene
ACLV7A_RS01095 (SMIE22_02120)	comGD/cglD	210762..211196 (+)	435	WP_411863364.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACLV7A_RS01100 (SMIE22_02130)	comGE/cglE	211159..211461 (+)	303	WP_000413380.1	competence type IV pilus minor pilin ComGE	Machinery gene
ACLV7A_RS01105 (SMIE22_02140)	comGF/cglF	211424..211885 (+)	462	WP_000250514.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACLV7A_RS01110 (SMIE22_02150)	comGG/cglG	211863..212276 (+)	414	WP_411863365.1	competence type IV pilus minor pilin ComGG	Machinery gene
ACLV7A_RS01115 (SMIE22_02160)	-	212309..212896 (+)	588	WP_411863366.1	class I SAM-dependent methyltransferase	-
ACLV7A_RS01120 (SMIE22_02170)	comYH	212957..213910 (+)	954	WP_411863367.1	class I SAM-dependent methyltransferase	Machinery gene
ACLV7A_RS01125 (SMIE22_02180)	-	213961..215151 (+)	1191	WP_000167777.1	acetate kinase	-
ACLV7A_RS01130 (SMIE22_02190)	rnpA	215299..215670 (+)	372	WP_000739245.1	ribonuclease P protein component	-
ACLV7A_RS01135 (SMIE22_02200)	-	215645..216469 (+)	825	WP_218757961.1	membrane protein insertase YidC	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17868.50 Da
• Isoelectric Point: 8.8053

>NTDB_id=1170257 ACLV7A_RS01105 WP_000250514.1 211424..211885(+) (comGF/cglF) [Streptococcus mitis isolate S. mitis E22]
MVQNSCWLSKSHKVKAFTLLESLIALIVISGSLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKEIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVRFRFQFQKGLEREFIYRVEKEKS

Nucleotide

• Length: 462 bp

>NTDB_id=1170257 ACLV7A_RS01105 WP_000250514.1 211424..211885(+) (comGF/cglF) [Streptococcus mitis isolate S. mitis E22]
ATGGTTCAGAACAGTTGTTGGCTATCAAAGAGCCATAAGGTCAAGGCTTTCACCTTGTTGGAATCCCTTATTGCTCTTAT
TGTCATCAGTGGAAGCTTACTCCTCTTTCAAGCCATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAGCTGGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTCTATATGAAGCAGGATGGCAAGGAAATTGCGATAGGTAAGTCTAAATCGGACGATTTTCGGAAAACCGATGCCAG
TGGACGGGGTTATCAACCTATGGTTTATGGACTCAAATCTGCACAGATTACAGAGGATAATCAACTGGTTCGCTTTCGTT
TCCAGTTCCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

3D structure

• AlphaFold DB: E9FQG6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus mitis NCTC 12261	96.078	100	0.961
comGF/cglF	Streptococcus mitis SK321	96.078	100	0.961
comGF/cglF	Streptococcus pneumoniae Rx1	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae D39	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae R6	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae TIGR4	93.464	100	0.935
comGF	Lactococcus lactis subsp. cremoris KW2	40	91.503	0.366