Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   ACLV7A_RS01085 Genome accession   NZ_OZ217344
Coordinates   209458..210474 (+) Length   338 a.a.
NCBI ID   WP_411864201.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis E22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 204458..215474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7A_RS01065 (SMIE22_02060) nagA 205607..206758 (+) 1152 WP_218757951.1 N-acetylglucosamine-6-phosphate deacetylase -
  ACLV7A_RS01070 (SMIE22_02070) - 206921..207979 (+) 1059 WP_084886478.1 zinc-dependent alcohol dehydrogenase family protein -
  ACLV7A_RS01075 (SMIE22_02080) - 208129..208494 (+) 366 WP_045606653.1 DUF1033 family protein -
  ACLV7A_RS01080 (SMIE22_02090) comGA/cglA/cilD 208569..209510 (+) 942 WP_411863363.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLV7A_RS01085 (SMIE22_02100) comGB/cglB 209458..210474 (+) 1017 WP_411864201.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLV7A_RS01090 (SMIE22_02110) comGC/cglC 210476..210799 (+) 324 WP_000738649.1 competence type IV pilus major pilin ComGC Machinery gene
  ACLV7A_RS01095 (SMIE22_02120) comGD/cglD 210762..211196 (+) 435 WP_411863364.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLV7A_RS01100 (SMIE22_02130) comGE/cglE 211159..211461 (+) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACLV7A_RS01105 (SMIE22_02140) comGF/cglF 211424..211885 (+) 462 WP_000250514.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLV7A_RS01110 (SMIE22_02150) comGG/cglG 211863..212276 (+) 414 WP_411863365.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLV7A_RS01115 (SMIE22_02160) - 212309..212896 (+) 588 WP_411863366.1 class I SAM-dependent methyltransferase -
  ACLV7A_RS01120 (SMIE22_02170) comYH 212957..213910 (+) 954 WP_411863367.1 class I SAM-dependent methyltransferase Machinery gene
  ACLV7A_RS01125 (SMIE22_02180) - 213961..215151 (+) 1191 WP_000167777.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38499.54 Da        Isoelectric Point: 9.5998

>NTDB_id=1170253 ACLV7A_RS01085 WP_411864201.1 209458..210474(+) (comGB/cglB) [Streptococcus mitis isolate S. mitis E22]
MDISQVFRLKRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVAQMRMGLSQGKSFSEMMDSLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGFISVGVLLALTFYKRSSKMRVFSILARLPFFGIFVQTYLTAYYAREWGNMISQGMELTQIFQIMQEQGSQLFKEI
GQDLAQSLQNGREFSQTIATYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFIRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1170253 ACLV7A_RS01085 WP_411864201.1 209458..210474(+) (comGB/cglB) [Streptococcus mitis isolate S. mitis E22]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCTTGTTTAA
CAATCTCTTTTCCAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCCTTGTTGGACAAGCAGTGTG
TGGCTCAGATGCGCATGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGACAGTTTGGGCTTTTCAAGTGCCATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGTAATCTTCACCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
TGCCAAGGTCAAGAAAAAACTAATTGAGGTAGCGACCTATCCCTTGATTTTGCTAGGTTTTCTTCTCTTAATCATGCTGG
GGCTACGGAACTACCTACTACCACAACTGGATAGTAGCAATATTGCCACCCAAATCATTGGTAATCTGCCACAAATCTTT
CTAGGAATGGTAGGGTTTATTTCAGTAGGTGTCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGCGCGTCTT
TTCTATCCTAGCACGCCTTCCCTTTTTTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTACGCGCGTGAGTGGGGGA
ATATGATTTCGCAGGGAATGGAGCTGACGCAGATTTTTCAAATCATGCAGGAACAAGGTTCCCAACTCTTTAAAGAAATC
GGCCAAGACCTAGCTCAATCCCTACAAAATGGCCGCGAATTTTCTCAAACCATAGCAACCTATCCTTTCTTTAAGAAAGA
GTTGAGTCTTATTATCGAGTATGGTGAAGTCAAGTCCAAGCTGGGGAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTATCCGAGTTAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

96.154

100

0.962
  comGB/cglB Streptococcus mitis NCTC 12261

96.154

100

0.962
  comGB/cglB Streptococcus pneumoniae Rx1

94.675

100

0.947
  comGB/cglB Streptococcus pneumoniae D39

94.675

100

0.947
  comGB/cglB Streptococcus pneumoniae R6

94.675

100

0.947
  comGB/cglB Streptococcus pneumoniae TIGR4

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

72.917

99.408

0.725
  comYB Streptococcus mutans UA140

58.934

94.379

0.556
  comYB Streptococcus mutans UA159

58.934

94.379

0.556
  comGB Lactococcus lactis subsp. cremoris KW2

50.599

98.817

0.5

Multiple sequence alignment