Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   ACLV65_RS01230 Genome accession   NZ_OZ217342
Coordinates   230065..231006 (+) Length   313 a.a.
NCBI ID   WP_411864325.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis B22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 225065..236006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV65_RS01210 (SMIB22_02350) - 225504..226787 (-) 1284 WP_411864323.1 DNA cytosine methyltransferase -
  ACLV65_RS01215 (SMIB22_02360) nagA 227099..228250 (+) 1152 WP_001134549.1 N-acetylglucosamine-6-phosphate deacetylase -
  ACLV65_RS01220 (SMIB22_02370) - 228413..229471 (+) 1059 WP_411864324.1 zinc-dependent alcohol dehydrogenase family protein -
  ACLV65_RS01225 (SMIB22_02380) - 229622..229987 (+) 366 WP_000286405.1 DUF1033 family protein -
  ACLV65_RS01230 (SMIB22_02390) comGA/cglA/cilD 230065..231006 (+) 942 WP_411864325.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLV65_RS01235 (SMIB22_02400) comGB/cglB 230954..231970 (+) 1017 WP_411865197.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLV65_RS01240 (SMIB22_02410) comGC/cglC 231972..232295 (+) 324 WP_172925580.1 competence type IV pilus major pilin ComGC Machinery gene
  ACLV65_RS01245 (SMIB22_02420) comGD/cglD 232258..232692 (+) 435 WP_411864326.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLV65_RS01250 (SMIB22_02430) comGE/cglE 232655..232957 (+) 303 WP_411864327.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACLV65_RS01255 (SMIB22_02440) comGF/cglF 232920..233381 (+) 462 WP_173234886.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLV65_RS01260 (SMIB22_02450) comGG/cglG 233359..233772 (+) 414 WP_411864328.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLV65_RS01265 (SMIB22_02460) - 233805..234392 (+) 588 WP_411864329.1 class I SAM-dependent methyltransferase -
  ACLV65_RS01270 (SMIB22_02470) comYH 234453..235406 (+) 954 WP_411864330.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35563.48 Da        Isoelectric Point: 5.9649

>NTDB_id=1170084 ACLV65_RS01230 WP_411864325.1 230065..231006(+) (comGA/cglA/cilD) [Streptococcus mitis isolate S. mitis B22]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGCYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIDELGKQYRHRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELTVVLQGVCYQRLIGGGGIVDFANKDYQEHQSTSWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1170084 ACLV65_RS01230 WP_411864325.1 230065..231006(+) (comGA/cglA/cilD) [Streptococcus mitis isolate S. mitis B22]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTTGTTATGATTTTGAAAAGTTTGCGGCCG
TCATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAGCGACGTAGCCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCATCTCTACGTCTATCTACTGTAGGCGATTATCGAGGGCATGAGAGTTTAGTCATTCGCTTGTT
GCATGATGAGGAGCAAGATTTACATTTTTGGTTTCAGGATATTGACGAATTGGGCAAGCAGTACAGGCACCGTGGTCTCT
ATCTCTTTGCTGGCCCAGTCGGCAGTGGCAAGACAACCTTGATGCACGAATTGGCCAAGTCCCTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGGTTGAC
CTATGAAAATCTAATCAAACTTTCTTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGAGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCCAGTTTGACAGGGGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATTCGAGGTGTTTAT
GAGCGCTTGCTGGAGTTGGGTGTGAGTGAGGAGGAATTAACAGTCGTTCTGCAAGGTGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAAGAACACCAGTCCACTAGCTGGAATGAACAGATTGACCAGC
TTCTTAAAGATGGACATATCACAAGCCTTCAAGCTGAAACGGAAAAAATTAGCTATAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

98.083

100

0.981
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae D39

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae R6

95.847

100

0.958
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

95.847

100

0.958
  comYA Streptococcus gordonii str. Challis substr. CH1

78.71

99.042

0.78
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361

Multiple sequence alignment