Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   ACLV65_RS01235 Genome accession   NZ_OZ217342
Coordinates   230954..231970 (+) Length   338 a.a.
NCBI ID   WP_411865197.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis B22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 225954..236970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV65_RS01215 (SMIB22_02360) nagA 227099..228250 (+) 1152 WP_001134549.1 N-acetylglucosamine-6-phosphate deacetylase -
  ACLV65_RS01220 (SMIB22_02370) - 228413..229471 (+) 1059 WP_411864324.1 zinc-dependent alcohol dehydrogenase family protein -
  ACLV65_RS01225 (SMIB22_02380) - 229622..229987 (+) 366 WP_000286405.1 DUF1033 family protein -
  ACLV65_RS01230 (SMIB22_02390) comGA/cglA/cilD 230065..231006 (+) 942 WP_411864325.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLV65_RS01235 (SMIB22_02400) comGB/cglB 230954..231970 (+) 1017 WP_411865197.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLV65_RS01240 (SMIB22_02410) comGC/cglC 231972..232295 (+) 324 WP_172925580.1 competence type IV pilus major pilin ComGC Machinery gene
  ACLV65_RS01245 (SMIB22_02420) comGD/cglD 232258..232692 (+) 435 WP_411864326.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLV65_RS01250 (SMIB22_02430) comGE/cglE 232655..232957 (+) 303 WP_411864327.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACLV65_RS01255 (SMIB22_02440) comGF/cglF 232920..233381 (+) 462 WP_173234886.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLV65_RS01260 (SMIB22_02450) comGG/cglG 233359..233772 (+) 414 WP_411864328.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLV65_RS01265 (SMIB22_02460) - 233805..234392 (+) 588 WP_411864329.1 class I SAM-dependent methyltransferase -
  ACLV65_RS01270 (SMIB22_02470) comYH 234453..235406 (+) 954 WP_411864330.1 class I SAM-dependent methyltransferase Machinery gene
  ACLV65_RS01275 (SMIB22_02480) - 235456..236646 (+) 1191 WP_411864331.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38492.54 Da        Isoelectric Point: 9.5925

>NTDB_id=1170085 ACLV65_RS01235 WP_411865197.1 230954..231970(+) (comGB/cglB) [Streptococcus mitis isolate S. mitis B22]
MDISQAFKLKRKKLAIAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRTGLSQGKSFSEMMESLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGFVSILALLALTFYKRSSKMRVFSILARIPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFHMMQEQGSQLFKEI
GQDLAQSLQNGREFSQTIATYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRINRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1170085 ACLV65_RS01235 WP_411865197.1 230954..231970(+) (comGB/cglB) [Streptococcus mitis isolate S. mitis B22]
ATGGACATATCACAAGCCTTCAAGCTGAAACGGAAAAAATTAGCTATAGCTAAGCAAAAAAATATCATCACCTTGTTTAA
CAATCTCTTTTCCAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCCTTGTTGGACAAGCAGTGTG
TGACTCAGATGCGTACAGGCTTGTCTCAGGGCAAATCATTCTCAGAAATGATGGAAAGTTTGGGCTTTTCAAGTGCCATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTAGGAAAGATAGAAGAATATCTGGATAATCT
GGCCAAGGTCAAGAAAAAACTAATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAACTACCTACTACCACAACTGGATAGTAGCAATATTGCCACCCAAATCATTGGCAATCTGCCACAAATTTTT
CTAGGAATGGTAGGGTTTGTTTCCATACTTGCCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGCGCGTCTT
TTCTATCCTAGCACGCATTCCTTTTCTTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTATGCGCGTGAGTGGGGGA
ATATGATTTCGCAGGGAATGGAGTTGACGCAGATTTTTCACATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGACTTGGCTCAGTCCCTACAAAATGGTCGCGAATTTTCTCAAACCATAGCAACCTATCCTTTCTTTAAGAAGGA
GTTGAGTCTCATCATCGAGTATGGGGAAGTCAAGTCCAAGCTGGGTAGTGAGTTGGAAATTTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAATTAACCGCACCATGAATTTAGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

96.746

100

0.967
  comGB/cglB Streptococcus mitis NCTC 12261

95.266

100

0.953
  comGB/cglB Streptococcus pneumoniae Rx1

94.675

100

0.947
  comGB/cglB Streptococcus pneumoniae D39

94.675

100

0.947
  comGB/cglB Streptococcus pneumoniae R6

94.675

100

0.947
  comGB/cglB Streptococcus pneumoniae TIGR4

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

72.024

99.408

0.716
  comYB Streptococcus mutans UA140

57.595

93.491

0.538
  comYB Streptococcus mutans UA159

57.595

93.491

0.538
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment