Detailed information NTG1170089

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: ACLV65_RS01255
• Genome accession: NZ_OZ217342
• Coordinates: 232920..233381 (+)
• Length: 153 a.a.
• NCBI ID: WP_173234886.1
• Uniprot ID: -
• Organism: Streptococcus mitis isolate S. mitis B22
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 227920..238381

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLV65_RS01220 (SMIB22_02370)	-	228413..229471 (+)	1059	WP_411864324.1	zinc-dependent alcohol dehydrogenase family protein	-
ACLV65_RS01225 (SMIB22_02380)	-	229622..229987 (+)	366	WP_000286405.1	DUF1033 family protein	-
ACLV65_RS01230 (SMIB22_02390)	comGA/cglA/cilD	230065..231006 (+)	942	WP_411864325.1	competence type IV pilus ATPase ComGA	Machinery gene
ACLV65_RS01235 (SMIB22_02400)	comGB/cglB	230954..231970 (+)	1017	WP_411865197.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACLV65_RS01240 (SMIB22_02410)	comGC/cglC	231972..232295 (+)	324	WP_172925580.1	competence type IV pilus major pilin ComGC	Machinery gene
ACLV65_RS01245 (SMIB22_02420)	comGD/cglD	232258..232692 (+)	435	WP_411864326.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACLV65_RS01250 (SMIB22_02430)	comGE/cglE	232655..232957 (+)	303	WP_411864327.1	competence type IV pilus minor pilin ComGE	Machinery gene
ACLV65_RS01255 (SMIB22_02440)	comGF/cglF	232920..233381 (+)	462	WP_173234886.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACLV65_RS01260 (SMIB22_02450)	comGG/cglG	233359..233772 (+)	414	WP_411864328.1	competence type IV pilus minor pilin ComGG	Machinery gene
ACLV65_RS01265 (SMIB22_02460)	-	233805..234392 (+)	588	WP_411864329.1	class I SAM-dependent methyltransferase	-
ACLV65_RS01270 (SMIB22_02470)	comYH	234453..235406 (+)	954	WP_411864330.1	class I SAM-dependent methyltransferase	Machinery gene
ACLV65_RS01275 (SMIB22_02480)	-	235456..236646 (+)	1191	WP_411864331.1	acetate kinase	-
ACLV65_RS01280 (SMIB22_02490)	rnpA	236796..237167 (+)	372	WP_173282963.1	ribonuclease P protein component	-
ACLV65_RS01285 (SMIB22_02500)	-	237142..237966 (+)	825	WP_200893270.1	membrane protein insertase YidC	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17781.43 Da
• Isoelectric Point: 9.6093

>NTDB_id=1170089 ACLV65_RS01255 WP_173234886.1 232920..233381(+) (comGF/cglF) [Streptococcus mitis isolate S. mitis B22]
MVQNSCWLSKSHKVKAFTLLESLIALIVISGSLLLFQAMSQLLISEVRYQQQSEQKEWILFVDQLEAELNRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQVVRFRFQFQKGLEREFIYRVEKAKS

Nucleotide

• Length: 462 bp

>NTDB_id=1170089 ACLV65_RS01255 WP_173234886.1 232920..233381(+) (comGF/cglF) [Streptococcus mitis isolate S. mitis B22]
ATGGTTCAGAACAGTTGTTGGCTATCAAAGAGTCATAAGGTCAAGGCTTTCACCTTGTTGGAATCCCTGATTGCCCTCAT
TGTCATCAGTGGAAGTTTACTCCTATTTCAGGCCATGAGTCAGCTCCTCATTTCAGAAGTTCGTTACCAGCAGCAAAGCG
AGCAAAAAGAGTGGATCTTGTTTGTGGATCAGTTGGAGGCGGAATTAAATCGTTCGCAATTCGAAAAAGTGGAGGGCAAT
CGCTTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGGAAAACCGATGCCAG
TGGACGGGGTTATCAACCTATGGTTTATGGCCTCAAATCCGCTCAAATTACAGAGGACAATCAAGTGGTTCGTTTTCGTT
TCCAGTTTCAGAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGCAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus mitis NCTC 12261	95.425	100	0.954
comGF/cglF	Streptococcus mitis SK321	95.425	100	0.954
comGF/cglF	Streptococcus pneumoniae Rx1	91.503	100	0.915
comGF/cglF	Streptococcus pneumoniae D39	91.503	100	0.915
comGF/cglF	Streptococcus pneumoniae R6	91.503	100	0.915
comGF/cglF	Streptococcus pneumoniae TIGR4	91.503	100	0.915