Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   ACLV7Q_RS01055 Genome accession   NZ_OZ217341
Coordinates   200612..201553 (+) Length   313 a.a.
NCBI ID   WP_218757953.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis C22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 195612..206553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7Q_RS01035 (SMIC22_01970) - 195679..197496 (+) 1818 WP_218757950.1 acyltransferase family protein -
  ACLV7Q_RS01040 (SMIC22_01980) nagA 197648..198799 (+) 1152 WP_218757951.1 N-acetylglucosamine-6-phosphate deacetylase -
  ACLV7Q_RS01045 (SMIC22_01990) - 198962..200020 (+) 1059 WP_218757952.1 zinc-dependent alcohol dehydrogenase family protein -
  ACLV7Q_RS01050 (SMIC22_02000) - 200170..200535 (+) 366 WP_045606653.1 DUF1033 family protein -
  ACLV7Q_RS01055 (SMIC22_02010) comGA/cglA/cilD 200612..201553 (+) 942 WP_218757953.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLV7Q_RS01060 (SMIC22_02020) comGB/cglB 201501..202517 (+) 1017 WP_218757970.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLV7Q_RS01065 (SMIC22_02030) comGC/cglC 202519..202842 (+) 324 WP_218757954.1 competence type IV pilus major pilin ComGC Machinery gene
  ACLV7Q_RS01070 (SMIC22_02040) comGD/cglD 202805..203239 (+) 435 WP_218757955.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLV7Q_RS01075 (SMIC22_02050) comGE/cglE 203202..203504 (+) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACLV7Q_RS01080 (SMIC22_02060) comGF/cglF 203467..203928 (+) 462 WP_218757956.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLV7Q_RS01085 (SMIC22_02070) comGG/cglG 203906..204319 (+) 414 WP_218757957.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLV7Q_RS01090 (SMIC22_02080) - 204352..204939 (+) 588 WP_218757958.1 class I SAM-dependent methyltransferase -
  ACLV7Q_RS01095 (SMIC22_02090) comYH 205000..205953 (+) 954 WP_218757959.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35405.36 Da        Isoelectric Point: 6.0055

>NTDB_id=1170002 ACLV7Q_RS01055 WP_218757953.1 200612..201553(+) (comGA/cglA/cilD) [Streptococcus mitis isolate S. mitis C22]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGCYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDDKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIGELGKQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANKGYQEHQPTSWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1170002 ACLV7Q_RS01055 WP_218757953.1 200612..201553(+) (comGA/cglA/cilD) [Streptococcus mitis isolate S. mitis C22]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGACGCCTATGAGCTTCATATGAGGGTCGGAGACGAGCGCTGTAAAATCGGTTGTTATGATTTTGAAAAGTTTGCGGCCG
TTATCAGTCACTTTAAGTTTGTAGCTGGTATGAATGTTGGAGAAAAGCGACGTAGTCAACTTGGTTCCTGCGATTATGCC
TATGACGATAAGATGGCGTCACTACGTTTGTCCACCGTTGGCGATTATCGAGGTCATGAGAGTTTAGTTATTCGCTTGTT
GCACGATGAGGAGCAGGATTTGCATTTTTGGTTTCAGGATATTGGAGAACTGGGTAAGCAGTATAGGCAACGGGGACTTT
ATCTCTTTGCTGGTCCAGTCGGCAGTGGTAAGACGACCTTGATGCATGAATTGGCCAAGTCTCTCTTTAAAGGACAGCAA
GTCATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTCCAATTACAGTTGAATGAAGCGATCGGGTTGAC
CTATGAAAATCTAATCAAACTTTCCTTGCGCCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGATAGCGAAACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGGGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATTCGAGGTGTTTAT
GAGCGCCTGCTGGAGTTGGGTGTGAGTGAGGAGGAATTGGCAGTCGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGGCTATCAAGAACACCAGCCAACTAGCTGGAATGAACAGATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

97.764

100

0.978
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

96.166

100

0.962
  comGA/cglA/cilD Streptococcus pneumoniae D39

96.166

100

0.962
  comGA/cglA/cilD Streptococcus pneumoniae R6

96.166

100

0.962
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

96.166

100

0.962
  comYA Streptococcus gordonii str. Challis substr. CH1

79.355

99.042

0.786
  comYA Streptococcus mutans UA159

65.273

99.361

0.649
  comYA Streptococcus mutans UA140

65.273

99.361

0.649
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

55.449

99.681

0.553
  comGA Latilactobacillus sakei subsp. sakei 23K

42.647

86.901

0.371

Multiple sequence alignment