Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   ACLV7Q_RS01060 Genome accession   NZ_OZ217341
Coordinates   201501..202517 (+) Length   338 a.a.
NCBI ID   WP_218757970.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis C22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 196501..207517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7Q_RS01040 (SMIC22_01980) nagA 197648..198799 (+) 1152 WP_218757951.1 N-acetylglucosamine-6-phosphate deacetylase -
  ACLV7Q_RS01045 (SMIC22_01990) - 198962..200020 (+) 1059 WP_218757952.1 zinc-dependent alcohol dehydrogenase family protein -
  ACLV7Q_RS01050 (SMIC22_02000) - 200170..200535 (+) 366 WP_045606653.1 DUF1033 family protein -
  ACLV7Q_RS01055 (SMIC22_02010) comGA/cglA/cilD 200612..201553 (+) 942 WP_218757953.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLV7Q_RS01060 (SMIC22_02020) comGB/cglB 201501..202517 (+) 1017 WP_218757970.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLV7Q_RS01065 (SMIC22_02030) comGC/cglC 202519..202842 (+) 324 WP_218757954.1 competence type IV pilus major pilin ComGC Machinery gene
  ACLV7Q_RS01070 (SMIC22_02040) comGD/cglD 202805..203239 (+) 435 WP_218757955.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLV7Q_RS01075 (SMIC22_02050) comGE/cglE 203202..203504 (+) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACLV7Q_RS01080 (SMIC22_02060) comGF/cglF 203467..203928 (+) 462 WP_218757956.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLV7Q_RS01085 (SMIC22_02070) comGG/cglG 203906..204319 (+) 414 WP_218757957.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLV7Q_RS01090 (SMIC22_02080) - 204352..204939 (+) 588 WP_218757958.1 class I SAM-dependent methyltransferase -
  ACLV7Q_RS01095 (SMIC22_02090) comYH 205000..205953 (+) 954 WP_218757959.1 class I SAM-dependent methyltransferase Machinery gene
  ACLV7Q_RS01100 (SMIC22_02100) - 206004..207194 (+) 1191 WP_218757960.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38497.52 Da        Isoelectric Point: 9.5998

>NTDB_id=1170003 ACLV7Q_RS01060 WP_218757970.1 201501..202517(+) (comGB/cglB) [Streptococcus mitis isolate S. mitis C22]
MDISQVFRLKRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRTGLSQGKSFSEMMESLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGFVSILALLALTFYKRSSKMRVFSILARIPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQALQNGREFSQTIATYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1170003 ACLV7Q_RS01060 WP_218757970.1 201501..202517(+) (comGB/cglB) [Streptococcus mitis isolate S. mitis C22]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATTACTTTGTTTAA
CAATCTCTTTTCCAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGAAAATCATTCTCAGAAATGATGGAAAGTTTGGGTTTTTCAAGTGCCATT
GTCACTCAGTTATCCCTAGCGGAAGTCCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
AGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCCTTGATTTTACTGGGATTTCTTCTCTTAATCATGCTGG
GGCTACGAAACTATCTACTACCACAACTGGATAGTAGCAATATTGCCACTCAAATTATCGGTAATCTCCCACAAATCTTT
CTAGGCATGGTAGGGTTTGTTTCCATACTTGCCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGCGCGTCTT
TTCTATCCTAGCACGCATTCCTTTTCTGGGAATCTTTGTGCAGACTTATCTGACAGCCTATTATGCGCGTGAATGGGGAA
ATATGATTTCGCAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATTTAGCTCAAGCCCTACAAAATGGTCGCGAATTTTCTCAAACCATAGCAACCTATCCTTTCTTTAAAAAGGA
GTTGAGTCTCATCATCGAGTATGGGGAAGTCAAGTCCAAGCTGGGGAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCTCTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

97.929

100

0.979
  comGB/cglB Streptococcus mitis NCTC 12261

97.041

100

0.97
  comGB/cglB Streptococcus pneumoniae Rx1

96.154

100

0.962
  comGB/cglB Streptococcus pneumoniae D39

96.154

100

0.962
  comGB/cglB Streptococcus pneumoniae R6

96.154

100

0.962
  comGB/cglB Streptococcus pneumoniae TIGR4

96.154

100

0.962
  comYB Streptococcus gordonii str. Challis substr. CH1

72.917

99.408

0.725
  comYB Streptococcus mutans UA140

57.911

93.491

0.541
  comYB Streptococcus mutans UA159

57.911

93.491

0.541
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment