Detailed information NTG1170007

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: ACLV7Q_RS01080
• Genome accession: NZ_OZ217341
• Coordinates: 203467..203928 (+)
• Length: 153 a.a.
• NCBI ID: WP_218757956.1
• Uniprot ID: -
• Organism: Streptococcus mitis isolate S. mitis C22
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 198467..208928

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLV7Q_RS01045 (SMIC22_01990)	-	198962..200020 (+)	1059	WP_218757952.1	zinc-dependent alcohol dehydrogenase family protein	-
ACLV7Q_RS01050 (SMIC22_02000)	-	200170..200535 (+)	366	WP_045606653.1	DUF1033 family protein	-
ACLV7Q_RS01055 (SMIC22_02010)	comGA/cglA/cilD	200612..201553 (+)	942	WP_218757953.1	competence type IV pilus ATPase ComGA	Machinery gene
ACLV7Q_RS01060 (SMIC22_02020)	comGB/cglB	201501..202517 (+)	1017	WP_218757970.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACLV7Q_RS01065 (SMIC22_02030)	comGC/cglC	202519..202842 (+)	324	WP_218757954.1	competence type IV pilus major pilin ComGC	Machinery gene
ACLV7Q_RS01070 (SMIC22_02040)	comGD/cglD	202805..203239 (+)	435	WP_218757955.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACLV7Q_RS01075 (SMIC22_02050)	comGE/cglE	203202..203504 (+)	303	WP_000413380.1	competence type IV pilus minor pilin ComGE	Machinery gene
ACLV7Q_RS01080 (SMIC22_02060)	comGF/cglF	203467..203928 (+)	462	WP_218757956.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACLV7Q_RS01085 (SMIC22_02070)	comGG/cglG	203906..204319 (+)	414	WP_218757957.1	competence type IV pilus minor pilin ComGG	Machinery gene
ACLV7Q_RS01090 (SMIC22_02080)	-	204352..204939 (+)	588	WP_218757958.1	class I SAM-dependent methyltransferase	-
ACLV7Q_RS01095 (SMIC22_02090)	comYH	205000..205953 (+)	954	WP_218757959.1	class I SAM-dependent methyltransferase	Machinery gene
ACLV7Q_RS01100 (SMIC22_02100)	-	206004..207194 (+)	1191	WP_218757960.1	acetate kinase	-
ACLV7Q_RS01105 (SMIC22_02110)	rnpA	207344..207715 (+)	372	WP_000739245.1	ribonuclease P protein component	-
ACLV7Q_RS01110 (SMIC22_02120)	-	207690..208514 (+)	825	WP_218757961.1	membrane protein insertase YidC	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17891.54 Da
• Isoelectric Point: 8.8086

>NTDB_id=1170007 ACLV7Q_RS01080 WP_218757956.1 203467..203928(+) (comGF/cglF) [Streptococcus mitis isolate S. mitis C22]
MVQNSCWLSKSHKIKAFTLLESLIALIVISGSLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAHITEENQLVRFRFQFQKGLEREFIYRVEKEKS

Nucleotide

• Length: 462 bp

>NTDB_id=1170007 ACLV7Q_RS01080 WP_218757956.1 203467..203928(+) (comGF/cglF) [Streptococcus mitis isolate S. mitis C22]
ATGGTTCAGAACAGTTGTTGGCTATCAAAGAGCCATAAGATCAAGGCCTTTACCTTGTTGGAATCTCTGATTGCTCTCAT
TGTCATCAGTGGAAGCTTACTTCTCTTTCAAGCCATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AACAAAAAGAGTGGCTCTTGTTTGTGGACCAGTTGGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTGGAGGGCAAT
CGCCTCTATATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCCAG
TGGACGGGGTTATCAGCCTATGGTTTATGGACTCAAATCAGCACATATTACAGAGGAAAATCAACTGGTGCGTTTTCGTT
TCCAGTTCCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus mitis NCTC 12261	96.078	100	0.961
comGF/cglF	Streptococcus mitis SK321	94.771	100	0.948
comGF/cglF	Streptococcus pneumoniae Rx1	92.81	100	0.928
comGF/cglF	Streptococcus pneumoniae D39	92.81	100	0.928
comGF/cglF	Streptococcus pneumoniae R6	92.81	100	0.928
comGF/cglF	Streptococcus pneumoniae TIGR4	92.81	100	0.928
comGF	Lactococcus lactis subsp. cremoris KW2	41.429	91.503	0.379