Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QMX71_RS14630 Genome accession   NZ_OX458332
Coordinates   3132611..3133729 (-) Length   372 a.a.
NCBI ID   WP_017365684.1    Uniprot ID   -
Organism   Methylococcus capsulatus isolate Mc(Nor)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3127611..3138729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMX71_RS14610 (MCNOR_3069) - 3128916..3129698 (+) 783 WP_010960814.1 succinate dehydrogenase iron-sulfur subunit -
  QMX71_RS14615 (MCNOR_3070) - 3129695..3129946 (+) 252 WP_017365687.1 succinate dehydrogenase assembly factor 2 -
  QMX71_RS14620 (MCNOR_3071) - 3129940..3130362 (+) 423 WP_017365686.1 protein YgfX -
  QMX71_RS14625 (MCNOR_3072) - 3130373..3132166 (+) 1794 WP_154656727.1 VWA domain-containing protein -
  QMX71_RS14630 (MCNOR_3074) pilU 3132611..3133729 (-) 1119 WP_017365684.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QMX71_RS14635 (MCNOR_3075) pilT 3133745..3134779 (-) 1035 WP_010960809.1 type IV pilus twitching motility protein PilT Machinery gene
  QMX71_RS14640 (MCNOR_3076) - 3134835..3135524 (+) 690 WP_282213351.1 YggS family pyridoxal phosphate-dependent enzyme -
  QMX71_RS14645 (MCNOR_3077) proC 3135595..3136419 (+) 825 WP_017365682.1 pyrroline-5-carboxylate reductase -
  QMX71_RS14650 (MCNOR_3078) - 3136423..3137001 (+) 579 WP_010960806.1 YggT family protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41711.05 Da        Isoelectric Point: 6.5905

>NTDB_id=1158454 QMX71_RS14630 WP_017365684.1 3132611..3133729(-) (pilU) [Methylococcus capsulatus isolate Mc(Nor)]
MEFESLLKLMVLKKASDLFITAAKEPCMKLNGTIVPLSSTKLTTDQVRQLVLGIMNQRQRDEFENTNECNFALSAAGLGR
FRVSAFVQRNSPGMVLRRIETEIPTVEQLNLPSVLNDLVMTKRGLILFVGATGTGKSTSLAAMLKYRNEHSSGHIITIED
PLEFVHPHAGCIVTQREVGIDTESYEVALKNTLRQAPDVILIGEIRTRETMQQAITFAETGHLCLSTLHANNANQALDRI
LHFFPEDMHPQVFMDLSLNLRAIIAQQLVRRADGKGRYPAVEILINTPLVSDLIRQGEVHKLKDVMKQSREQGMQTFDQA
LFELFKAGRIGYEDALYSADSKNEVRLMIKLSEEGSIDKYAPKDDSIRIVDN

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1158454 QMX71_RS14630 WP_017365684.1 3132611..3133729(-) (pilU) [Methylococcus capsulatus isolate Mc(Nor)]
ATGGAATTCGAATCGCTGCTCAAGCTGATGGTACTGAAGAAAGCATCCGATCTCTTCATCACCGCGGCCAAGGAGCCCTG
CATGAAGCTGAACGGCACCATCGTGCCGCTGTCCAGCACCAAACTCACCACCGACCAGGTGCGCCAGCTCGTCCTCGGGA
TCATGAACCAGCGCCAGCGCGACGAGTTCGAGAACACCAACGAATGCAACTTCGCCCTCTCCGCGGCCGGACTGGGCCGG
TTCCGGGTCAGCGCCTTCGTGCAGCGGAACAGTCCCGGCATGGTGCTGCGCAGGATCGAGACCGAGATTCCCACCGTGGA
ACAGCTCAATCTGCCCTCCGTCCTCAACGACCTGGTCATGACCAAGCGCGGCCTGATCCTGTTCGTGGGCGCGACCGGCA
CCGGCAAGTCGACGTCGCTGGCGGCGATGCTCAAGTACCGCAACGAGCACTCCAGCGGCCACATCATCACCATCGAGGAT
CCCCTGGAATTCGTACACCCGCACGCGGGCTGCATCGTCACCCAGCGCGAGGTCGGCATCGATACCGAATCCTACGAAGT
CGCGCTGAAGAACACCCTGCGGCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGCACCCGCGAGACCATGCAGCAGG
CCATCACCTTCGCCGAAACCGGCCACCTCTGCCTGAGCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTCCATTTCTTCCCGGAAGACATGCATCCGCAGGTGTTCATGGACCTGTCGCTGAACCTGCGTGCCATCATCGCCCAGCA
GCTCGTCAGGCGCGCCGACGGCAAGGGGCGCTATCCGGCGGTGGAAATCCTCATCAATACCCCTCTGGTCTCCGACCTGA
TCCGCCAGGGCGAGGTCCACAAGCTCAAGGACGTGATGAAACAGTCGCGCGAACAGGGCATGCAGACCTTCGACCAGGCG
CTGTTCGAATTGTTCAAGGCGGGCAGGATCGGCTACGAGGATGCCCTCTACTCCGCCGATTCCAAGAACGAAGTCCGCTT
GATGATCAAGCTCAGCGAAGAAGGCAGCATCGACAAATACGCGCCCAAGGACGACTCGATCCGGATCGTCGACAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.492

95.161

0.661
  pilU Acinetobacter baylyi ADP1

64.706

95.968

0.621
  pilU Vibrio cholerae strain A1552

58

94.086

0.546
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.103

93.548

0.403
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.617

89.785

0.374
  pilT Vibrio cholerae strain A1552

41.617

89.785

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.204

0.371
  pilT Legionella pneumophila strain Lp02

40.233

92.204

0.371
  pilT Acinetobacter baylyi ADP1

39.883

91.667

0.366
  pilT Pseudomonas aeruginosa PAK

40.597

90.054

0.366
  pilT Pseudomonas stutzeri DSM 10701

40

90.054

0.36
  pilT Acinetobacter nosocomialis M2

39.528

91.129

0.36
  pilT Acinetobacter baumannii D1279779

39.528

91.129

0.36
  pilT Acinetobacter baumannii strain A118

39.528

91.129

0.36

Multiple sequence alignment