Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CKW05_RS10885 Genome accession   NZ_LT906457
Coordinates   2412950..2414068 (-) Length   372 a.a.
NCBI ID   WP_058482153.1    Uniprot ID   A0A0W0ZBI6
Organism   Legionella spiritensis strain NCTC11990     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2407950..2419068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW05_RS10860 (SAMEA4412668_02168) - 2407973..2408704 (-) 732 WP_058482158.1 RES family NAD+ phosphorylase -
  CKW05_RS10865 (SAMEA4412668_02169) - 2408701..2409075 (-) 375 WP_058482157.1 MbcA/ParS/Xre antitoxin family protein -
  CKW05_RS10870 (SAMEA4412668_02170) - 2409427..2410290 (-) 864 WP_156413330.1 hypothetical protein -
  CKW05_RS10880 (SAMEA4412668_02171) ankK 2410642..2412684 (-) 2043 WP_058482154.1 Dot/Icm T4SS effector AnkK/LegA5 -
  CKW05_RS10885 (SAMEA4412668_02172) pilU 2412950..2414068 (-) 1119 WP_058482153.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CKW05_RS10890 (SAMEA4412668_02173) - 2414283..2414858 (+) 576 WP_058482152.1 hypothetical protein -
  CKW05_RS10895 (SAMEA4412668_02174) - 2414877..2415410 (+) 534 WP_133141189.1 hypothetical protein -
  CKW05_RS10900 (SAMEA4412668_02175) - 2415388..2417937 (+) 2550 WP_058482150.1 PD-(D/E)XK nuclease family protein -
  CKW05_RS10905 (SAMEA4412668_02176) - 2417941..2418798 (+) 858 WP_065238351.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41811.09 Da        Isoelectric Point: 5.0944

>NTDB_id=1147579 CKW05_RS10885 WP_058482153.1 2412950..2414068(-) (pilU) [Legionella spiritensis strain NCTC11990]
MDITPFFKLMVDRGASDLFFSVGAPPNIKIEGITSPIGQTPLKAQQMAEIAASVMDDDQRKEFEATMELNMAISLGGVGR
FRVNMFRQRGEIAMVVRYIKGIIPSIEELNLPPILNTIVMELRGLILVVGSTGSGKSTTLASMIDYRNENQRGHILTIED
PIEYLYRHKKSIVDQREVGIDTLNYENALKNAMREAPDMIMIGEVRDRGTMKHAIAYAETGHLCLSTLHANNANQTLDRI
LNFFPEDARHQILLDLSLNLRAIISLRLIPGLENQRVPAVEVMINSPYISDLIEKGKVDEIKEVMSRSREQGMQTFDQAL
FDLYKAGKISKENAIRFADSKNNVGLQIRLSEERGIDGDVDLTIEEDEKNKF

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1147579 CKW05_RS10885 WP_058482153.1 2412950..2414068(-) (pilU) [Legionella spiritensis strain NCTC11990]
ATGGATATAACGCCCTTTTTTAAGCTGATGGTAGACCGAGGCGCTTCTGATTTATTTTTCAGCGTGGGTGCTCCTCCTAA
CATCAAAATTGAAGGGATCACGTCTCCAATTGGACAAACTCCTTTAAAAGCGCAACAAATGGCAGAAATAGCGGCGTCTG
TTATGGACGATGATCAGCGCAAGGAGTTTGAAGCGACTATGGAATTGAATATGGCCATTTCCCTTGGCGGGGTGGGACGT
TTCAGGGTCAATATGTTTCGGCAACGTGGTGAGATCGCTATGGTGGTACGCTACATCAAAGGAATTATCCCGTCGATTGA
GGAGCTGAATTTACCGCCCATTCTCAATACCATTGTGATGGAGTTACGAGGTTTGATCCTGGTGGTTGGGTCGACCGGTT
CCGGTAAATCAACCACTCTCGCCTCAATGATTGATTACCGCAATGAAAACCAGCGCGGGCATATATTAACTATTGAAGAT
CCTATCGAGTATTTGTACCGTCACAAAAAATCGATTGTCGATCAGCGGGAAGTGGGCATTGATACCTTAAATTACGAAAA
CGCGCTTAAAAACGCCATGCGTGAGGCGCCTGACATGATTATGATTGGTGAGGTAAGGGATAGAGGCACCATGAAACACG
CCATTGCCTATGCGGAAACAGGGCATTTATGTTTATCCACGCTTCATGCCAACAATGCCAACCAGACCCTGGACAGGATC
CTGAATTTCTTTCCGGAAGACGCCAGACACCAGATCCTGCTTGATTTATCCCTTAATTTAAGGGCAATCATTTCATTACG
GTTAATTCCTGGACTTGAGAACCAACGTGTTCCGGCCGTGGAGGTGATGATTAATTCACCTTATATCTCCGATTTGATTG
AAAAAGGCAAAGTGGACGAAATTAAGGAAGTTATGTCAAGAAGCCGCGAGCAGGGGATGCAGACGTTTGATCAGGCGTTA
TTCGATCTCTACAAAGCCGGTAAAATCAGTAAAGAGAATGCGATTCGATTCGCAGATTCGAAAAATAATGTGGGCTTGCA
AATTCGTTTGTCCGAAGAGCGTGGAATCGATGGCGATGTTGATTTAACCATCGAGGAAGACGAAAAAAATAAATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W0ZBI6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

52.958

95.43

0.505
  pilU Pseudomonas stutzeri DSM 10701

53.107

95.161

0.505
  pilU Vibrio cholerae strain A1552

48.883

96.237

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

91.129

0.395
  pilT Pseudomonas aeruginosa PAK

42.814

89.785

0.384
  pilT Neisseria meningitidis 8013

41.21

93.28

0.384
  pilT Neisseria gonorrhoeae MS11

41.21

93.28

0.384
  pilT Pseudomonas stutzeri DSM 10701

41.108

92.204

0.379
  pilT Legionella pneumophila strain ERS1305867

41.617

89.785

0.374
  pilT Legionella pneumophila strain Lp02

41.617

89.785

0.374
  pilT Acinetobacter baylyi ADP1

40.588

91.398

0.371
  pilT Acinetobacter nosocomialis M2

41.018

89.785

0.368
  pilT Acinetobacter baumannii D1279779

41.018

89.785

0.368
  pilT Acinetobacter baumannii strain A118

41.018

89.785

0.368

Multiple sequence alignment