Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   CFBP1590_RS04975 Genome accession   NZ_LT855380
Coordinates   1029823..1030260 (+) Length   145 a.a.
NCBI ID   WP_025993964.1    Uniprot ID   A0A1Y6JFS9
Organism   Pseudomonas viridiflava strain CFBP 1590 isolate E12-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 1030783..1033439 1029823..1030260 flank 523


Gene organization within MGE regions


Location: 1029823..1033439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFBP1590_RS04975 (CFBP1590__1048) pilA2 1029823..1030260 (+) 438 WP_025993964.1 pilin Machinery gene
  CFBP1590_RS04980 (CFBP1590__1049) - 1030783..1031505 (-) 723 Protein_962 IS66 family transposase -
  CFBP1590_RS04985 (CFBP1590__1050) - 1031550..1033061 (-) 1512 WP_088234579.1 IS66 family transposase -
  CFBP1590_RS04990 (CFBP1590__1051) tnpB 1033080..1033439 (-) 360 WP_004883347.1 IS66 family insertion sequence element accessory protein TnpB -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14901.06 Da        Isoelectric Point: 9.0030

>NTDB_id=1146529 CFBP1590_RS04975 WP_025993964.1 1029823..1030260(+) (pilA2) [Pseudomonas viridiflava strain CFBP 1590 isolate E12-5]
MKVQKTLQKGFTLIELMIVVAIVGILAAVAIPAYQDYTIRAQVAELATLADGAKVAVSETYQTTGAFPTSNATANYGGAS
GTYTTSVAIGAGGVITATTINGAPVNNAIRNQTFVLTPVLGTNNSLGWTCTAGTIVAQYRPANCR

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1146529 CFBP1590_RS04975 WP_025993964.1 1029823..1030260(+) (pilA2) [Pseudomonas viridiflava strain CFBP 1590 isolate E12-5]
ATGAAAGTACAAAAAACGCTACAAAAAGGTTTTACGCTGATCGAACTGATGATCGTCGTAGCGATCGTGGGCATTCTCGC
AGCGGTGGCCATTCCTGCATATCAGGACTACACAATCCGCGCTCAGGTAGCAGAGCTGGCGACGCTTGCGGATGGTGCCA
AGGTCGCGGTTAGCGAGACTTACCAGACTACCGGCGCTTTTCCTACAAGCAACGCTACTGCAAACTACGGTGGTGCGTCT
GGTACTTACACTACCTCCGTTGCAATTGGAGCTGGCGGAGTTATCACCGCAACTACAATTAACGGAGCACCCGTGAATAA
TGCCATTCGTAACCAGACTTTTGTACTGACTCCGGTTCTAGGAACAAACAACTCTCTTGGCTGGACATGTACTGCAGGTA
CAATCGTCGCTCAATATCGTCCAGCCAACTGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-145)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-30)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y6JFS9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

58.273

95.862

0.559
  pilA2 Legionella pneumophila strain ERS1305867

57.554

95.862

0.552
  pilA Ralstonia pseudosolanacearum GMI1000

38.636

100

0.469
  pilE Neisseria gonorrhoeae MS11

50.388

88.966

0.448
  pilE Neisseria gonorrhoeae strain FA1090

50.781

88.276

0.448
  pilA/pilA1 Eikenella corrodens VA1

39.241

100

0.428
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

33.155

100

0.428
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.789

100

0.428
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.541

100

0.414
  pilA Vibrio campbellii strain DS40M4

45.113

91.724

0.414
  comP Acinetobacter baylyi ADP1

40.69

100

0.407
  pilA Acinetobacter baumannii strain A118

36.735

100

0.372
  pilA Haemophilus influenzae Rd KW20

37.063

98.621

0.366
  pilA Haemophilus influenzae 86-028NP

36.301

100

0.366

Multiple sequence alignment