Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NBC2815_RS16035 Genome accession   NZ_LT853880
Coordinates   3461391..3461807 (-) Length   138 a.a.
NCBI ID   WP_002812272.1    Uniprot ID   A0A1Y6GZ11
Organism   Xanthomonas fragariae strain NBC2815     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3456391..3466807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBC2815_RS16005 (NBC2815_03195) - 3456580..3456762 (+) 183 WP_088061932.1 YhfG family protein -
  NBC2815_RS16010 (NBC2815_03196) - 3456859..3457356 (+) 498 WP_231895734.1 Fic family protein -
  NBC2815_RS16015 (NBC2815_03197) - 3457427..3458794 (-) 1368 WP_088062623.1 IS5 family transposase -
  NBC2815_RS16025 (NBC2815_03198) pilB 3459072..3460808 (-) 1737 WP_002812268.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NBC2815_RS16030 - 3460878..3461309 (-) 432 WP_088061936.1 pilin -
  NBC2815_RS16035 (NBC2815_03199) pilA 3461391..3461807 (-) 417 WP_002812272.1 pilin Machinery gene
  NBC2815_RS16040 (NBC2815_03200) pilC 3462158..3463414 (+) 1257 WP_088061938.1 type II secretion system F family protein Machinery gene
  NBC2815_RS16045 (NBC2815_03201) - 3463421..3464284 (+) 864 WP_088061940.1 A24 family peptidase -
  NBC2815_RS16050 (NBC2815_03202) coaE 3464298..3464909 (+) 612 WP_002812280.1 dephospho-CoA kinase -
  NBC2815_RS16055 (NBC2815_03203) - 3465669..3466794 (-) 1126 Protein_3127 IS3 family transposase -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14395.66 Da        Isoelectric Point: 8.4815

>NTDB_id=1146429 NBC2815_RS16035 WP_002812272.1 3461391..3461807(-) (pilA) [Xanthomonas fragariae strain NBC2815]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQNYVAKSQVAAGLAEITPGKTQYEVMVNEGKASTITPDLIGLKTSTRCTI
STPSPSETGAITCALLNTSPKVAGQSISWTRAADGTWTCSTTLSATDKPKYAPATCQG

Nucleotide


Download         Length: 417 bp        

>NTDB_id=1146429 NBC2815_RS16035 WP_002812272.1 3461391..3461807(-) (pilA) [Xanthomonas fragariae strain NBC2815]
ATGAAGAAGCAACAAGGCTTTACGTTGATTGAACTGATGATCGTGGTTGCCATCATTGCCATCCTGGCAGCAATTGCTCT
CCCTGCTTATCAGAATTATGTTGCCAAGTCGCAGGTCGCTGCAGGTCTGGCGGAAATCACTCCTGGTAAAACTCAGTATG
AAGTCATGGTCAACGAAGGTAAGGCTTCCACGATTACTCCTGATCTTATCGGTCTGAAGACGTCCACCCGCTGCACGATT
AGCACTCCGAGCCCGAGCGAGACTGGTGCAATCACCTGCGCTCTGCTGAACACAAGTCCTAAGGTTGCTGGTCAGAGCAT
CTCTTGGACGCGTGCTGCAGATGGTACGTGGACTTGCTCGACCACTTTGAGCGCCACTGACAAGCCCAAGTATGCACCGG
CAACCTGCCAGGGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-137)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y6GZ11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

50.35

100

0.522
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50

100

0.5
  pilA Pseudomonas aeruginosa PAK

43.151

100

0.457
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.588

98.551

0.449
  pilA Ralstonia pseudosolanacearum GMI1000

35.119

100

0.428
  comP Acinetobacter baylyi ADP1

38.411

100

0.42
  pilA Vibrio cholerae C6706

41.304

100

0.413
  pilA Vibrio cholerae strain A1552

41.304

100

0.413
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.304

100

0.413
  pilA Vibrio parahaemolyticus RIMD 2210633

41.667

95.652

0.399
  pilE Neisseria gonorrhoeae strain FA1090

34.615

100

0.391
  pilA2 Legionella pneumophila str. Paris

35.333

100

0.384
  pilA2 Legionella pneumophila strain ERS1305867

35.333

100

0.384

Multiple sequence alignment