Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   O987_RS25150 Genome accession   NZ_CP006704
Coordinates   5559180..5560316 (+) Length   378 a.a.
NCBI ID   WP_043375486.1    Uniprot ID   A0A076PX65
Organism   Comamonas testosteroni TK102     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5554180..5565316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O987_RS25130 (O987_26090) - 5555014..5555784 (+) 771 WP_019043137.1 TetR/AcrR family transcriptional regulator -
  O987_RS25135 (O987_26095) - 5555831..5557078 (+) 1248 WP_043375482.1 branched-chain amino acid ABC transporter substrate-binding protein -
  O987_RS25140 (O987_26100) - 5557244..5557975 (-) 732 WP_043375484.1 YggS family pyridoxal phosphate-dependent enzyme -
  O987_RS25145 (O987_26105) pilT 5558013..5559056 (+) 1044 WP_003050584.1 type IV pilus twitching motility protein PilT Machinery gene
  O987_RS25150 (O987_26110) pilU 5559180..5560316 (+) 1137 WP_043375486.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  O987_RS25155 (O987_26115) - 5560465..5561376 (+) 912 WP_003050579.1 NAD(P)-dependent oxidoreductase -
  O987_RS25160 (O987_26120) - 5561438..5562091 (-) 654 WP_043375488.1 BON domain-containing protein -
  O987_RS25165 (O987_26125) - 5562088..5562696 (-) 609 WP_043375490.1 SIS domain-containing protein -
  O987_RS25170 (O987_26130) - 5562786..5563187 (-) 402 WP_043375492.1 YraN family protein -
  O987_RS25175 (O987_26135) rsmI 5563187..5564110 (+) 924 WP_043375494.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41932.44 Da        Isoelectric Point: 6.9661

>NTDB_id=113602 O987_RS25150 WP_043375486.1 5559180..5560316(+) (pilU) [Comamonas testosteroni TK102]
MERGQASKFINDLLKLMVSRNGSDLFITAEFPPAIKIDGKVTKVSPQPLSPLHTLALARSIMNDRQVADFEHTKECNFAI
SPAGVGRFRVNAFMQQGKVGMVLRTIPTTLPTIDGLGVPQVLKEVTMTKRGLCVLVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIVNFFPEERRPQLLMDVSLNLRAIISQRLIPKQDSKGRVAAIEVMLNSPLIADLIFKGEVVEIKEIMKKSRNIG
MQTFDQALFDLFESNLITYEDALRNADSVNDLRLQIKLNSQRARTLDLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=113602 O987_RS25150 WP_043375486.1 5559180..5560316(+) (pilU) [Comamonas testosteroni TK102]
ATGGAACGCGGTCAGGCCAGTAAATTCATCAACGATCTGCTCAAGCTCATGGTCAGCCGCAACGGCAGCGACCTGTTCAT
CACGGCGGAGTTTCCGCCCGCCATCAAGATTGACGGCAAAGTCACCAAGGTCTCGCCCCAGCCGCTGAGCCCGCTGCATA
CGCTGGCCCTGGCACGCTCCATCATGAACGACAGGCAGGTGGCGGACTTCGAGCACACCAAGGAGTGCAACTTCGCCATC
TCGCCGGCCGGCGTGGGTCGCTTCCGCGTCAATGCCTTCATGCAGCAGGGCAAGGTCGGCATGGTGCTGCGTACCATTCC
CACCACCCTGCCCACGATTGACGGCCTGGGCGTGCCGCAGGTGCTCAAGGAAGTGACCATGACCAAGCGCGGCCTGTGCG
TCCTGGTGGGCGCGACGGGCTCGGGCAAGTCCACCACGCTGGCTGCCATGGTGGACTGGCGCAATGAGAACTCCTTCGGC
CACATCATCACGGTGGAAGACCCCGTGGAATTCGTGCACCCGCACAAGAACTGCGTAGTCACGCAACGCGAGGTGGGGCT
GGATACGGACAGCTGGGAAGCGGCACTCAAGAACACGCTGCGCCAGGCTCCCGATGTGATTTTGATGGGCGAAATCCGCG
ACCGTGAAACCATGGAGCACGCGATTGCGTTCTCGGAAACCGGTCACCTGTGCCTGGCCACGCTGCATGCCAACAGTGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTTCCCGAAGAACGCCGTCCACAGCTGCTGATGGATGTGTCGCTGAACCT
GCGGGCCATCATCTCCCAGCGTCTGATTCCCAAGCAGGACAGCAAGGGCCGTGTCGCCGCCATCGAGGTGATGCTCAACT
CTCCGCTGATTGCCGACCTGATCTTCAAGGGCGAGGTCGTCGAGATCAAGGAGATCATGAAGAAAAGCCGCAATATCGGC
ATGCAGACCTTCGATCAGGCGCTGTTCGACCTGTTCGAATCCAATCTCATCACCTACGAAGATGCGCTGCGCAATGCAGA
CTCGGTCAACGACCTGCGTCTGCAGATCAAGCTCAACAGCCAGCGCGCCCGGACTCTGGACCTGGCAGCCGGCACCGAAC
ATCTGACCATCGTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A076PX65

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.749

96.825

0.598
  pilU Acinetobacter baylyi ADP1

55.989

94.974

0.532
  pilU Vibrio cholerae strain A1552

54.023

92.063

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter baumannii D1279779

42.943

88.095

0.378
  pilT Acinetobacter baumannii strain A118

42.943

88.095

0.378
  pilT Acinetobacter nosocomialis M2

42.943

88.095

0.378
  pilT Legionella pneumophila strain ERS1305867

42.643

88.095

0.376
  pilT Legionella pneumophila strain Lp02

42.643

88.095

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362
  pilT Acinetobacter baylyi ADP1

41.768

86.772

0.362

Multiple sequence alignment