Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CFBP8129_RS07055 Genome accession   NZ_LR828257
Coordinates   1664316..1665449 (-) Length   377 a.a.
NCBI ID   WP_006449645.1    Uniprot ID   A0A0G8M6N0
Organism   Xanthomonas hortorum pv. vitians strain CFBP 498     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1659316..1670449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFBP8129_RS07040 (CFBP498_14520) - 1660321..1661709 (-) 1389 WP_006450465.1 DEAD/DEAH box helicase -
  CFBP8129_RS07045 (CFBP498_14530) - 1662018..1663076 (+) 1059 WP_172839291.1 fumarylacetoacetate hydrolase family protein -
  CFBP8129_RS07050 (CFBP498_14540) maiA 1663132..1663794 (+) 663 WP_074056612.1 maleylacetoacetate isomerase -
  CFBP8129_RS07055 (CFBP498_14550) pilU 1664316..1665449 (-) 1134 WP_006449645.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CFBP8129_RS07060 (CFBP498_14560) - 1665548..1665931 (+) 384 WP_006449646.1 DUF4398 domain-containing protein -
  CFBP8129_RS07065 (CFBP498_14570) - 1665928..1666818 (+) 891 WP_074056613.1 hypothetical protein -
  CFBP8129_RS07070 (CFBP498_14580) - 1667164..1667382 (+) 219 WP_002814034.1 YdcH family protein -
  CFBP8129_RS07075 (CFBP498_14590) - 1667601..1668971 (+) 1371 WP_074056614.1 pyridoxal-phosphate dependent enzyme -
  CFBP8129_RS07080 (CFBP498_14600) - 1669074..1670273 (+) 1200 WP_095574960.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41755.01 Da        Isoelectric Point: 6.1395

>NTDB_id=1132397 CFBP8129_RS07055 WP_006449645.1 1664316..1665449(-) (pilU) [Xanthomonas hortorum pv. vitians strain CFBP 498]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMAISLPDAGR
FRVNVFKQRGEAGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLLVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHTFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGQDGRRRPATEVLLNTPMIRDLLRRGQVHEVKAAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETAAAPKISHGFV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1132397 CFBP8129_RS07055 WP_006449645.1 1664316..1665449(-) (pilU) [Xanthomonas hortorum pv. vitians strain CFBP 498]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTTTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCCTACTCGT
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAACTCAACATGGCCATCTCGCTGCCCGACGCCGGACGC
TTCCGTGTGAACGTGTTCAAGCAGCGTGGCGAAGCAGGCATGGTGATCCGCGCCATCCGCAGCCGGATCCCGAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACGCCGCGCGGTCTGGTACTGCTGGTCGGCTCGACCGGTT
CCGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAAGTCGGCCTGGATACCCACACCTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAAATCCTGGATGCCGAGACCATGGAGGCGG
CGATTGCATTTGCCGAAACCGGTCACCTGTGTCTGGCGACGCTGCATTCCAACAATGCCGATCAGACCATCGAGCGCATT
CTCAATTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAATCTGGCGCTGAATTTGCGTGCGGTGGTGTCGCAACG
CCTGGTCAAGGGCCAGGACGGACGCCGCCGCCCCGCCACCGAAGTCTTGCTCAACACCCCGATGATTCGCGACCTGCTGC
GACGCGGCCAGGTGCACGAGGTCAAGGCGGCGATGGAAGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGCATGGTTAAATCAGGTGAAATCGAACAGGAGGAAGCACTGCGCGCCGCCGACTCGCGCGATGGTCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGGCTCCGGCGAACACGACCCGTATGCGGATTACGAGACCGCCGCAGCGCCCAAGATCAGCC
ACGGGTTTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8M6N0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.925

95.225

0.504
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.455

100

0.44
  pilT Pseudomonas aeruginosa PAK

42.899

89.655

0.385
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Acinetobacter baumannii strain A118

41.42

89.655

0.371
  pilT Acinetobacter nosocomialis M2

41.42

89.655

0.371
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.42

89.655

0.371
  pilT Acinetobacter baylyi ADP1

40.882

90.186

0.369
  pilT Neisseria gonorrhoeae MS11

39.655

92.308

0.366
  pilT Neisseria meningitidis 8013

39.655

92.308

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.317

88.594

0.366

Multiple sequence alignment