Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L1X59_RS17615 Genome accession   NZ_LR813086
Coordinates   3724794..3725930 (+) Length   378 a.a.
NCBI ID   WP_045265948.1    Uniprot ID   A0A1B2D0T0
Organism   Comamonas aquatica isolate BB1454     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3719794..3730930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1X59_RS17595 (TML_03485) - 3721165..3721569 (-) 405 WP_234687130.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  L1X59_RS17600 (TML_03486) - 3721595..3722746 (-) 1152 WP_210929696.1 aminotransferase class V-fold PLP-dependent enzyme -
  L1X59_RS17605 (TML_03487) - 3722917..3723636 (-) 720 WP_234687131.1 YggS family pyridoxal phosphate-dependent enzyme -
  L1X59_RS17610 (TML_03488) pilT 3723726..3724769 (+) 1044 WP_043383872.1 type IV pilus twitching motility protein PilT Machinery gene
  L1X59_RS17615 (TML_03489) pilU 3724794..3725930 (+) 1137 WP_045265948.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L1X59_RS17620 (TML_03490) - 3725995..3726654 (-) 660 WP_045265947.1 BON domain-containing protein -
  L1X59_RS17625 (TML_03491) - 3726654..3727250 (-) 597 WP_042417407.1 SIS domain-containing protein -
  L1X59_RS17630 (TML_03492) - 3727365..3727763 (-) 399 WP_042417409.1 YraN family protein -
  L1X59_RS17635 (TML_03493) rsmI 3727763..3728686 (+) 924 WP_234687132.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  L1X59_RS17640 (TML_03494) - 3728796..3730046 (-) 1251 WP_234687133.1 Hsp70 family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42189.76 Da        Isoelectric Point: 6.4338

>NTDB_id=1130412 L1X59_RS17615 WP_045265948.1 3724794..3725930(+) (pilU) [Comamonas aquatica isolate BB1454]
MERDQASKFINDLLKLMVARKGSDLFITAEFPPAIKVDGKVTRLMDQPLVPTHTLMLARSIMSDKQVAEFERTKECNFAI
SPAGIGRFRVNAFIQQGRVGMVLRTIPADIPTIDGMGLPQVLKEVAMTKRGLCIFVGATGSGKSTSLAAMVDWRNEHSEG
HIVTVEDPIEFVHPHKNCIITQREVGLDTDSWEAALKNSLRQAPDVILMGEIRDRETMEHAIVFSETGHLCLATLHANNA
NQALDRIVNFFPEERREQLLMDLSLNLRAMISQRLLPKEGGQGRVAALEVMLNTPLISDLIAKGDVVEIKEIMKKSRELG
MQTFDQSLLDLYESGAVMYEDALRNADSVNDLRLQIKLHSKRAHNTDLAAGTEHFRIL

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1130412 L1X59_RS17615 WP_045265948.1 3724794..3725930(+) (pilU) [Comamonas aquatica isolate BB1454]
ATGGAACGCGATCAGGCCAGCAAATTCATCAACGACCTTCTCAAGCTGATGGTGGCGCGCAAGGGCAGCGACCTGTTCAT
CACCGCCGAATTTCCGCCCGCCATCAAGGTCGACGGCAAGGTCACACGGCTGATGGACCAGCCGCTGGTGCCCACGCACA
CCCTGATGCTGGCACGCTCCATCATGAGCGACAAGCAGGTGGCGGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCCATC
TCGCCCGCCGGCATCGGGCGTTTTCGCGTCAACGCCTTCATCCAGCAGGGCCGCGTGGGCATGGTGCTGCGGACCATTCC
GGCCGACATCCCCACCATCGACGGCATGGGCCTGCCCCAGGTGCTGAAGGAGGTAGCCATGACCAAGCGTGGCCTGTGCA
TCTTCGTGGGGGCCACCGGGTCGGGCAAGTCGACCTCGCTGGCGGCCATGGTGGACTGGCGCAACGAACACTCCGAGGGG
CATATCGTCACGGTGGAAGACCCCATCGAATTCGTGCACCCACACAAGAACTGCATCATCACCCAGCGCGAGGTGGGGCT
GGACACCGACAGCTGGGAGGCGGCGCTCAAGAACTCGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCACGCCATCGTGTTCTCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCC
AACCAGGCGCTGGACCGTATCGTCAACTTCTTCCCGGAAGAGCGCCGCGAGCAACTGCTGATGGACCTGTCGCTGAACCT
GCGCGCCATGATCTCGCAGCGCCTGCTGCCCAAAGAAGGCGGCCAGGGCCGTGTGGCCGCGCTGGAGGTGATGCTCAACA
CCCCACTGATCTCGGACCTGATCGCCAAGGGCGACGTGGTGGAAATCAAGGAAATCATGAAGAAAAGTCGCGAGCTGGGC
ATGCAGACCTTCGACCAGTCGCTGCTCGACCTCTACGAAAGCGGCGCCGTCATGTACGAGGACGCGTTGCGCAATGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTGCACAGCAAACGTGCCCACAACACCGACCTGGCGGCGGGTACCGAGC
ACTTCCGCATCCTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B2D0T0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.496

95.503

0.587
  pilU Acinetobacter baylyi ADP1

59.143

92.593

0.548
  pilU Vibrio cholerae strain A1552

55.46

92.063

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Acinetobacter baylyi ADP1

44.88

87.831

0.394
  pilT Pseudomonas stutzeri DSM 10701

43.402

90.212

0.392
  pilT Pseudomonas aeruginosa PAK

43.917

89.153

0.392
  pilT Acinetobacter nosocomialis M2

43.844

88.095

0.386
  pilT Acinetobacter baumannii D1279779

43.844

88.095

0.386
  pilT Acinetobacter baumannii strain A118

43.844

88.095

0.386
  pilT Legionella pneumophila strain ERS1305867

44.72

85.185

0.381
  pilT Legionella pneumophila strain Lp02

44.72

85.185

0.381
  pilT Vibrio cholerae strain A1552

43.293

86.772

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.293

86.772

0.376

Multiple sequence alignment