Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL239_RS05615 Genome accession   NZ_LR134374
Coordinates   1289411..1290529 (-) Length   372 a.a.
NCBI ID   WP_126338734.1    Uniprot ID   -
Organism   Legionella spiritensis strain NCTC12082     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1284411..1295529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL239_RS05600 (NCTC12082_01139) - 1285103..1285477 (-) 375 WP_126338731.1 MbcA/ParS/Xre antitoxin family protein -
  EL239_RS05605 (NCTC12082_01140) - 1285828..1286763 (-) 936 WP_126338732.1 hypothetical protein -
  EL239_RS05610 (NCTC12082_01141) ankK 1287103..1289145 (-) 2043 WP_126338733.1 Dot/Icm T4SS effector AnkK/LegA5 -
  EL239_RS05615 (NCTC12082_01142) pilU 1289411..1290529 (-) 1119 WP_126338734.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL239_RS05620 (NCTC12082_01143) - 1290744..1291319 (+) 576 WP_126338735.1 hypothetical protein -
  EL239_RS05625 (NCTC12082_01144) - 1291338..1291871 (+) 534 WP_126338736.1 hypothetical protein -
  EL239_RS05630 (NCTC12082_01145) - 1291849..1294398 (+) 2550 WP_126338737.1 PD-(D/E)XK nuclease family protein -
  EL239_RS05635 (NCTC12082_01146) - 1294402..1295259 (+) 858 WP_126338738.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41797.06 Da        Isoelectric Point: 5.0944

>NTDB_id=1122401 EL239_RS05615 WP_126338734.1 1289411..1290529(-) (pilU) [Legionella spiritensis strain NCTC12082]
MDITPFFKLMVDRGASDLFFSVGAPPNIKIEGITSPIGQTPLKAQQMAEIAASVMDDDQRKEFEATMELNMAVSLGGVGR
FRVNMFRQRGEIAMVVRYIKGIIPSIEELNLPPILNTIVMELRGLILVVGSTGSGKSTTLASMIDYRNENQRGHILTIED
PIEYLYRHKKSIVDQREVGIDTLNYENALKNAMREAPDMIMIGEVRDRGTMKHAIAYAETGHLCLSTLHANNANQTLDRI
LNFFPEDARHQILLDLSLNLRAIISLRLIPGLENQRVPAVEVMINSPYISDLIEKGKVDEIKEVMSRSREQGMQTFDQAL
FDLYKAGKISKENAIRFADSKNNVGLQIRLSEERGIDGDVDLTIEEDEKNKF

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1122401 EL239_RS05615 WP_126338734.1 1289411..1290529(-) (pilU) [Legionella spiritensis strain NCTC12082]
ATGGATATAACGCCCTTTTTTAAGCTGATGGTAGACCGAGGCGCCTCTGATTTATTTTTCAGCGTAGGCGCTCCTCCTAA
CATCAAAATTGAAGGGATCACGTCTCCAATTGGACAAACTCCTTTAAAAGCACAACAAATGGCAGAAATAGCGGCGTCTG
TTATGGACGATGATCAGCGCAAGGAGTTTGAAGCGACTATGGAATTGAATATGGCCGTTTCCCTTGGCGGGGTGGGACGT
TTCAGGGTCAATATGTTTCGGCAACGTGGTGAGATCGCTATGGTGGTACGCTACATCAAAGGAATTATCCCGTCGATTGA
GGAGCTGAATTTACCGCCCATTCTCAATACCATTGTGATGGAGTTACGAGGTTTGATCCTGGTGGTTGGGTCGACCGGTT
CCGGTAAATCAACCACTCTCGCCTCAATGATTGATTACCGCAATGAAAACCAGCGCGGGCATATATTAACTATTGAAGAT
CCTATCGAGTATTTGTACCGTCACAAAAAATCGATTGTCGATCAGCGGGAAGTGGGCATTGATACCTTAAATTACGAAAA
CGCGCTTAAAAACGCCATGCGTGAGGCGCCTGACATGATTATGATTGGTGAGGTAAGGGATAGAGGCACCATGAAACACG
CCATTGCCTATGCGGAAACAGGGCATTTATGTTTATCCACGCTTCATGCCAACAATGCCAACCAGACCCTGGACAGGATC
CTGAATTTCTTTCCGGAAGATGCCAGACACCAGATCCTGCTTGATTTATCCCTTAATTTAAGGGCAATCATTTCATTACG
GTTAATTCCTGGACTTGAGAACCAACGTGTTCCGGCCGTGGAGGTGATGATTAATTCACCTTATATCTCCGATTTGATTG
AAAAAGGCAAAGTGGACGAAATTAAGGAAGTTATGTCAAGAAGCCGCGAGCAGGGGATGCAGACGTTTGATCAGGCGTTA
TTCGATCTCTACAAAGCCGGTAAAATCAGTAAAGAGAATGCGATTCGATTCGCAGATTCGAAAAATAATGTGGGCTTGCA
AATTCGTTTATCCGAAGAGCGTGGAATCGATGGCGATGTTGATTTAACCATCGAGGAAGACGAAAAAAATAAATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.825

95.161

0.503
  pilU Acinetobacter baylyi ADP1

52.676

95.43

0.503
  pilU Vibrio cholerae strain A1552

49.162

96.237

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

91.129

0.395
  pilT Pseudomonas aeruginosa PAK

42.814

89.785

0.384
  pilT Neisseria meningitidis 8013

41.21

93.28

0.384
  pilT Neisseria gonorrhoeae MS11

41.21

93.28

0.384
  pilT Pseudomonas stutzeri DSM 10701

41.108

92.204

0.379
  pilT Legionella pneumophila strain ERS1305867

41.617

89.785

0.374
  pilT Legionella pneumophila strain Lp02

41.617

89.785

0.374
  pilT Acinetobacter baylyi ADP1

40.588

91.398

0.371
  pilT Acinetobacter nosocomialis M2

41.018

89.785

0.368
  pilT Acinetobacter baumannii D1279779

41.018

89.785

0.368
  pilT Acinetobacter baumannii strain A118

41.018

89.785

0.368

Multiple sequence alignment