Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   EL087_RS00990 Genome accession   NZ_LR134288
Coordinates   188212..189153 (+) Length   313 a.a.
NCBI ID   WP_126406231.1    Uniprot ID   -
Organism   Streptococcus milleri strain NCTC11169     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 183212..194153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL087_RS00965 (NCTC11169_00188) - 183528..184727 (+) 1200 WP_126406222.1 phosphoglycerate kinase -
  EL087_RS00970 (NCTC11169_00189) - 185242..185772 (+) 531 WP_126406225.1 FUSC family protein -
  EL087_RS00975 (NCTC11169_00190) - 185849..186208 (+) 360 WP_003028467.1 MerR family transcriptional regulator -
  EL087_RS00980 (NCTC11169_00191) glnA 186246..187592 (+) 1347 WP_106079126.1 type I glutamate--ammonia ligase -
  EL087_RS00985 (NCTC11169_00192) - 187777..188142 (+) 366 WP_126406228.1 DUF1033 family protein -
  EL087_RS00990 (NCTC11169_00193) comYA 188212..189153 (+) 942 WP_126406231.1 competence type IV pilus ATPase ComGA Machinery gene
  EL087_RS00995 (NCTC11169_00194) comYB 189095..190117 (+) 1023 WP_182465780.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL087_RS01000 (NCTC11169_00195) comYC 190114..190431 (+) 318 WP_003028473.1 competence type IV pilus major pilin ComGC Machinery gene
  EL087_RS01005 (NCTC11169_00196) comYD 190391..190819 (+) 429 WP_037566697.1 competence type IV pilus minor pilin ComGD Machinery gene
  EL087_RS01010 (NCTC11169_00197) comGE/cglE 190791..191084 (+) 294 WP_126406234.1 competence type IV pilus minor pilin ComGE Machinery gene
  EL087_RS01015 (NCTC11169_00198) comGF/cglF 191068..191505 (+) 438 WP_049513212.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL087_RS01020 (NCTC11169_00199) comGG 191486..191812 (+) 327 WP_269470973.1 competence type IV pilus minor pilin ComGG -
  EL087_RS01025 (NCTC11169_00200) comYH 191884..192837 (+) 954 WP_003039058.1 class I SAM-dependent methyltransferase Machinery gene
  EL087_RS01030 (NCTC11169_00201) - 192884..194083 (+) 1200 WP_126406237.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35780.03 Da        Isoelectric Point: 6.1753

>NTDB_id=1120230 EL087_RS00990 WP_126406231.1 188212..189153(+) (comYA) [Streptococcus milleri strain NCTC11169]
MVQNIAQAIICQAKKEQAQDIYVVPKENMYELYMRIGDERRFIQQYAFEEMAAVISHFKFTAGMNVGEKRRSQLGSCDYQ
YGDKKTSLRLSTVGDYRGYESLVIRLLHDEETELKFWFAQIPELKECFKQRGLYLFSGPVGSGKTTLMYQLAQERFAGQQ
IMSIEDPVEIKQPSMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDGETARAVVRASLTGATVFSTIHAKSVRGVY
ERLLELGVTEAELKIVLQGICYQRLIAKGGIVDFVSEKYQEHEPSDWNRQIDQLYAAGHINLEQAEAEKIIHS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1120230 EL087_RS00990 WP_126406231.1 188212..189153(+) (comYA) [Streptococcus milleri strain NCTC11169]
ATGGTTCAAAACATTGCACAGGCTATTATTTGCCAAGCTAAAAAAGAGCAGGCACAGGATATTTACGTTGTTCCAAAGGA
GAATATGTATGAGCTATACATGCGAATTGGCGATGAAAGGCGTTTTATCCAACAGTATGCATTTGAAGAGATGGCAGCAG
TAATTAGTCATTTCAAGTTTACCGCTGGGATGAATGTCGGCGAAAAACGTAGAAGCCAGCTAGGATCTTGTGATTATCAA
TATGGTGATAAGAAAACGTCATTACGACTGTCAACAGTAGGGGATTATCGTGGATATGAAAGTCTGGTGATTCGTTTATT
GCATGATGAAGAGACAGAGTTGAAGTTTTGGTTTGCTCAAATCCCAGAGTTAAAAGAATGCTTCAAACAACGAGGGCTTT
ATCTTTTTTCGGGACCAGTAGGGAGTGGAAAAACGACTCTCATGTACCAACTTGCCCAAGAGCGCTTTGCTGGTCAACAA
ATCATGTCCATTGAAGACCCTGTTGAAATCAAGCAGCCTAGCATGTTACAGTTGCAATTGAATGAAGCGATTGGTTTGAC
TTATGAAAATTTGATTAAACTTTCTCTACGGCACCGACCGGATTTGCTAATCATCGGAGAAATTCGAGATGGGGAGACAG
CACGGGCTGTTGTCCGAGCTAGCTTGACAGGAGCGACGGTCTTTTCAACCATTCATGCTAAAAGCGTGCGAGGTGTCTAT
GAACGGTTGTTGGAATTGGGAGTGACAGAAGCCGAGCTGAAAATTGTCTTGCAAGGGATTTGTTACCAACGTTTAATTGC
AAAAGGAGGAATAGTGGATTTTGTCAGTGAAAAGTATCAAGAACACGAGCCAAGCGACTGGAATCGACAGATTGATCAGC
TTTATGCAGCAGGACATATCAATCTTGAACAAGCAGAAGCGGAAAAAATTATCCACAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus gordonii str. Challis substr. CH1

83.226

99.042

0.824
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

76.038

100

0.76
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

75.719

100

0.757
  comGA/cglA/cilD Streptococcus pneumoniae D39

75.719

100

0.757
  comGA/cglA/cilD Streptococcus pneumoniae R6

75.719

100

0.757
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

75.719

100

0.757
  comYA Streptococcus mutans UA159

65.595

99.361

0.652
  comYA Streptococcus mutans UA140

65.595

99.361

0.652
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.341

99.361

0.54
  comGA Latilactobacillus sakei subsp. sakei 23K

41.115

91.693

0.377

Multiple sequence alignment