Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   EL087_RS00995 Genome accession   NZ_LR134288
Coordinates   189095..190117 (+) Length   340 a.a.
NCBI ID   WP_182465780.1    Uniprot ID   -
Organism   Streptococcus milleri strain NCTC11169     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 184095..195117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL087_RS00970 (NCTC11169_00189) - 185242..185772 (+) 531 WP_126406225.1 FUSC family protein -
  EL087_RS00975 (NCTC11169_00190) - 185849..186208 (+) 360 WP_003028467.1 MerR family transcriptional regulator -
  EL087_RS00980 (NCTC11169_00191) glnA 186246..187592 (+) 1347 WP_106079126.1 type I glutamate--ammonia ligase -
  EL087_RS00985 (NCTC11169_00192) - 187777..188142 (+) 366 WP_126406228.1 DUF1033 family protein -
  EL087_RS00990 (NCTC11169_00193) comYA 188212..189153 (+) 942 WP_126406231.1 competence type IV pilus ATPase ComGA Machinery gene
  EL087_RS00995 (NCTC11169_00194) comYB 189095..190117 (+) 1023 WP_182465780.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL087_RS01000 (NCTC11169_00195) comYC 190114..190431 (+) 318 WP_003028473.1 competence type IV pilus major pilin ComGC Machinery gene
  EL087_RS01005 (NCTC11169_00196) comYD 190391..190819 (+) 429 WP_037566697.1 competence type IV pilus minor pilin ComGD Machinery gene
  EL087_RS01010 (NCTC11169_00197) comGE/cglE 190791..191084 (+) 294 WP_126406234.1 competence type IV pilus minor pilin ComGE Machinery gene
  EL087_RS01015 (NCTC11169_00198) comGF/cglF 191068..191505 (+) 438 WP_049513212.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL087_RS01020 (NCTC11169_00199) comGG 191486..191812 (+) 327 WP_269470973.1 competence type IV pilus minor pilin ComGG -
  EL087_RS01025 (NCTC11169_00200) comYH 191884..192837 (+) 954 WP_003039058.1 class I SAM-dependent methyltransferase Machinery gene
  EL087_RS01030 (NCTC11169_00201) - 192884..194083 (+) 1200 WP_126406237.1 acetate kinase -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 39098.12 Da        Isoelectric Point: 9.7507

>NTDB_id=1120231 EL087_RS00995 WP_182465780.1 189095..190117(+) (comYB) [Streptococcus milleri strain NCTC11169]
MQQDISILNKQKRKKLSTVKQKKVVELLNNLFSSGFHLAEIIDFLKRSALLEEVYVDRMKEGLSTGKPFSDIMASLGFSD
NVVTQLSLAELHGNLSLSLSKIEEYLENVSKIKKKLVEVATYPFILFVFLIFIMLGLRNYLLPQLDRQNVATQIISNLPQ
LFLGSLGVVFVLLAIGFYWFQKNSKITTFGLLARLPFFGAFIQTYLTAYYAREWGNMIGQGLELSQIFQMMQGQRSAIFQ
EIGKDLEVALQNGQEFSQRVKTYPFFKKELGLMIEYGEVKSRLGRELEVYAQKTWETFFKRVHQAMNVIQPLVFVFVALM
IVLLYAAMLLPIYQNMEVQL

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=1120231 EL087_RS00995 WP_182465780.1 189095..190117(+) (comYB) [Streptococcus milleri strain NCTC11169]
ATGCAGCAGGACATATCAATCTTGAACAAGCAGAAGCGGAAAAAATTATCCACAGTTAAGCAAAAAAAGGTCGTAGAGTT
ACTGAATAATCTTTTTTCTAGTGGCTTTCATTTGGCAGAAATTATCGACTTTTTGAAGCGAAGTGCCTTGTTAGAAGAAG
TCTATGTGGACAGGATGAAAGAGGGTTTGTCAACAGGAAAGCCTTTTTCGGACATCATGGCAAGCTTAGGTTTTTCAGAT
AACGTGGTGACACAGCTTTCGCTGGCAGAATTACACGGAAATCTCTCACTCAGCCTGAGTAAGATTGAGGAATACTTGGA
AAATGTCTCAAAAATTAAGAAAAAGTTGGTTGAAGTGGCGACTTATCCCTTTATTTTGTTTGTTTTTTTGATATTCATTA
TGCTGGGATTACGTAATTATTTACTCCCGCAACTCGATCGTCAAAATGTAGCAACGCAGATTATCAGTAATTTGCCGCAA
CTTTTTCTGGGTTCATTAGGAGTAGTCTTTGTTTTGTTAGCGATAGGTTTTTATTGGTTCCAAAAAAACTCAAAAATAAC
AACATTTGGCTTGTTAGCTCGCCTTCCCTTTTTTGGCGCTTTTATTCAAACGTATTTGACAGCTTATTATGCGAGAGAAT
GGGGAAATATGATTGGTCAAGGTTTAGAACTTAGTCAGATTTTTCAGATGATGCAAGGACAACGCTCTGCTATATTTCAA
GAAATTGGGAAAGATTTAGAAGTTGCGCTGCAAAATGGTCAGGAATTTTCACAAAGGGTTAAAACATACCCATTTTTTAA
AAAGGAATTGGGTTTGATGATCGAATACGGCGAAGTGAAGTCTAGATTGGGGCGTGAATTAGAAGTTTATGCTCAAAAAA
CATGGGAGACATTTTTTAAACGTGTTCACCAAGCGATGAATGTCATCCAACCACTAGTGTTTGTCTTTGTGGCTTTAATG
ATTGTATTGTTGTATGCAGCCATGTTGCTGCCCATTTATCAAAATATGGAGGTTCAATTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(26-143)

Type II secretion system (T2SS), protein F (T2SSF)

(211-331)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus gordonii str. Challis substr. CH1

70.294

100

0.703
  comGB/cglB Streptococcus mitis NCTC 12261

67.761

98.529

0.668
  comGB/cglB Streptococcus mitis SK321

67.164

98.529

0.662
  comGB/cglB Streptococcus pneumoniae TIGR4

66.866

98.529

0.659
  comGB/cglB Streptococcus pneumoniae R6

66.866

98.529

0.659
  comGB/cglB Streptococcus pneumoniae Rx1

66.866

98.529

0.659
  comGB/cglB Streptococcus pneumoniae D39

66.866

98.529

0.659
  comYB Streptococcus mutans UA159

60.819

100

0.612
  comYB Streptococcus mutans UA140

60.526

100

0.609
  comGB Lactococcus lactis subsp. cremoris KW2

51.632

99.118

0.512

Multiple sequence alignment