Detailed information NTG1120235

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: EL087_RS01015
• Genome accession: NZ_LR134288
• Coordinates: 191068..191505 (+)
• Length: 145 a.a.
• NCBI ID: WP_049513212.1
• Uniprot ID: A0A4U9ZRD8
• Organism: Streptococcus milleri strain NCTC11169
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 186068..196505

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL087_RS00980 (NCTC11169_00191)	glnA	186246..187592 (+)	1347	WP_106079126.1	type I glutamate--ammonia ligase	-
EL087_RS00985 (NCTC11169_00192)	-	187777..188142 (+)	366	WP_126406228.1	DUF1033 family protein	-
EL087_RS00990 (NCTC11169_00193)	comYA	188212..189153 (+)	942	WP_126406231.1	competence type IV pilus ATPase ComGA	Machinery gene
EL087_RS00995 (NCTC11169_00194)	comYB	189095..190117 (+)	1023	WP_182465780.1	competence type IV pilus assembly protein ComGB	Machinery gene
EL087_RS01000 (NCTC11169_00195)	comYC	190114..190431 (+)	318	WP_003028473.1	competence type IV pilus major pilin ComGC	Machinery gene
EL087_RS01005 (NCTC11169_00196)	comYD	190391..190819 (+)	429	WP_037566697.1	competence type IV pilus minor pilin ComGD	Machinery gene
EL087_RS01010 (NCTC11169_00197)	comGE/cglE	190791..191084 (+)	294	WP_126406234.1	competence type IV pilus minor pilin ComGE	Machinery gene
EL087_RS01015 (NCTC11169_00198)	comGF/cglF	191068..191505 (+)	438	WP_049513212.1	competence type IV pilus minor pilin ComGF	Machinery gene
EL087_RS01020 (NCTC11169_00199)	comGG	191486..191812 (+)	327	WP_269470973.1	competence type IV pilus minor pilin ComGG	-
EL087_RS01025 (NCTC11169_00200)	comYH	191884..192837 (+)	954	WP_003039058.1	class I SAM-dependent methyltransferase	Machinery gene
EL087_RS01030 (NCTC11169_00201)	-	192884..194083 (+)	1200	WP_126406237.1	acetate kinase	-
EL087_RS01035 (NCTC11169_00202)	glmS	194470..196284 (+)	1815	WP_126406239.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16829.39 Da
• Isoelectric Point: 10.0941

>NTDB_id=1120235 EL087_RS01015 WP_049513212.1 191068..191505(+) (comGF/cglF) [Streptococcus milleri strain NCTC11169]
MFKANKVKAFTLLEALIALFVLSGSVLLFQSMTRLLAFEVQARQHSEQREWLLFADQLEAELARSSFEKVENNRLYMKQD
GKVIAFGKSSGQDFRKTNANGKGYQPMVYDVKKAEISQKNELIHVRLTFRRGLEREFVYRVETES

Nucleotide

• Length: 438 bp

>NTDB_id=1120235 EL087_RS01015 WP_049513212.1 191068..191505(+) (comGF/cglF) [Streptococcus milleri strain NCTC11169]
GTGTTCAAGGCAAATAAAGTGAAAGCTTTTACCCTTTTAGAGGCTCTGATTGCCTTATTTGTACTCAGCGGAAGTGTGCT
GCTTTTTCAGTCAATGACGCGTCTTCTGGCTTTTGAAGTTCAAGCCCGGCAACATAGTGAACAACGAGAATGGCTTTTGT
TTGCTGATCAACTAGAAGCAGAGTTGGCTCGTTCTTCATTTGAAAAGGTGGAGAATAATCGTTTATATATGAAACAGGAC
GGCAAGGTGATTGCATTTGGAAAGTCAAGTGGACAGGATTTTAGAAAAACCAATGCGAATGGGAAAGGTTACCAGCCGAT
GGTTTATGATGTGAAAAAAGCAGAAATCTCACAGAAAAATGAGCTGATTCATGTCAGACTGACGTTTAGGCGAGGATTGG
AAAGGGAGTTTGTCTATCGTGTGGAAACAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: A0A4U9ZRD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae R6	65.493	97.931	0.641
comGF/cglF	Streptococcus pneumoniae TIGR4	65.493	97.931	0.641
comGF/cglF	Streptococcus pneumoniae Rx1	65.493	97.931	0.641
comGF/cglF	Streptococcus pneumoniae D39	65.493	97.931	0.641
comGF/cglF	Streptococcus mitis NCTC 12261	65.714	96.552	0.634
comGF/cglF	Streptococcus mitis SK321	65.714	96.552	0.634
comYF	Streptococcus mutans UA159	43.704	93.103	0.407
comYF	Streptococcus mutans UA140	43.704	93.103	0.407
comGF	Lactococcus lactis subsp. cremoris KW2	41.481	93.103	0.386