Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KHO06_RS14260 Genome accession   NZ_HG992751
Coordinates   3085450..3086556 (-) Length   368 a.a.
NCBI ID   WP_005425812.1    Uniprot ID   A7MTP8
Organism   Vibrio sp. B1FIG11 isolate LMG10946-nano     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3080450..3091556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHO06_RS14230 (ACOMICROBIO_LMKGKHOH_00084) - 3081035..3081532 (+) 498 WP_212581779.1 SprT family zinc-dependent metalloprotease -
  KHO06_RS14235 (ACOMICROBIO_LOCUS1915) endA 3081683..3082378 (+) 696 WP_005425828.1 deoxyribonuclease I -
  KHO06_RS14240 (ACOMICROBIO_LOCUS1916) rsmE 3082525..3083256 (+) 732 WP_005425825.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  KHO06_RS14245 (ACOMICROBIO_LOCUS1917) gshB 3083271..3084222 (+) 952 Protein_2720 glutathione synthase -
  KHO06_RS14250 (ACOMICROBIO_LOCUS1918) - 3084352..3084915 (+) 564 WP_038892068.1 YqgE/AlgH family protein -
  KHO06_RS14255 (ACOMICROBIO_LOCUS1919) ruvX 3084963..3085388 (+) 426 WP_038892069.1 Holliday junction resolvase RuvX -
  KHO06_RS14260 (ACOMICROBIO_LOCUS1920) pilU 3085450..3086556 (-) 1107 WP_005425812.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KHO06_RS14265 (ACOMICROBIO_LOCUS1921) pilT 3086588..3087628 (-) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  KHO06_RS14270 (ACOMICROBIO_LMKGKHOH_00092) - 3087657..3088366 (+) 710 Protein_2725 YggS family pyridoxal phosphate-dependent enzyme -
  KHO06_RS14275 (ACOMICROBIO_LOCUS1922) proC 3088497..3089315 (+) 819 WP_212581781.1 pyrroline-5-carboxylate reductase -
  KHO06_RS14280 (ACOMICROBIO_LMKGKHOH_00094) - 3089368..3089925 (+) 558 WP_005425806.1 YggT family protein -
  KHO06_RS14285 (ACOMICROBIO_LMKGKHOH_00095) yggU 3089925..3090215 (+) 291 WP_005425805.1 DUF167 family protein YggU -
  KHO06_RS14290 (ACOMICROBIO_LMKGKHOH_00096) - 3090275..3090706 (+) 432 WP_005425804.1 DUF4426 domain-containing protein -
  KHO06_RS14295 (ACOMICROBIO_LOCUS1923) - 3090812..3091414 (+) 603 WP_005436525.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41335.48 Da        Isoelectric Point: 6.7731

>NTDB_id=1112490 KHO06_RS14260 WP_005425812.1 3085450..3086556(-) (pilU) [Vibrio sp. B1FIG11 isolate LMG10946-nano]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLTENDVALLLDSAMEPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNNNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGTLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1112490 KHO06_RS14260 WP_005425812.1 3085450..3086556(-) (pilU) [Vibrio sp. B1FIG11 isolate LMG10946-nano]
ATGGATCTGAATAAATTTCTCGAAGGCATGTTGGCGTTAAAAGCGTCGGATCTCTACATCACTGTCGGTGCTCCGATTTT
GTTTCGTGTGGATGGTGAATTGCGCCCGCAAGGCGATAAGCTGACTGAAAATGATGTCGCCTTGTTACTTGACAGTGCAA
TGGAACCAGAGCGACGCCAGGAGTTTCGTAAAAGCCGTGAGTCTAACTTTGCCATTGTCCGTGATTGCGGTCGTTTCCGT
GTCAGTGCCTTTTTCCAACGTGAATTACCGGGAGCCGTTATTCGTCGTATTGAAACCAATATTCCGACGTTTGAGCAACT
TAAGTTACCTTTGGTACTGCAAGATCTTGCTATTGCCAAGCGTGGTTTAGTGCTGGTGGTTGGTGCCACTGGTTCTGGTA
AGTCGACCACTATGGCGGCGATGACGGGTTATCGCAACAACAACAAAACGGGTCATATCCTGACGGTAGAAGACCCGATT
GAATTTGTGCACGAACATAAGCGTTGCATCGTGACACAGCGTGAAGTTGGCCTAGACACCGACAGCTATGAAGTGGCGTT
GAAAAACTCGTTACGTCAGGCGCCAGACATGATTTTGATTGGTGAGATCCGTAGCCGAGAAACGATGGAATACGCGATGA
CCTTTGCTGAGACTGGTCACTTGTGTATGGCGACACTGCACGCCAACAACGCAAACCAAGCGCTGGAGCGTATTTTGCAC
CTAGTGCCGAAAGATCAGAAAGATCAGTTCTTATTTGATCTGTCGATGAACTTAAAAGGTGTGGTGGGTCAGCAGTTGAT
CCGTGATAAGAACGGTCAGGGGCGTCATGGCGTGTTTGAGATCTTATTAAACAGTCCACGTGTTTCTGATCTGATCCGTC
GAGGTGATCTGCATGAATTGAAATCGACCATGGCTCGCTCTAACGAGTTCGGGATGTTGACCTTCGACCAATCGCTCTAC
AAGTTGGTGATGCAAGGTAAGATTAGCGAAGAGGATGCATTGCACAGTGCAGATTCAGCCAACGATCTGCGTTTGATGTT
GAAGACGCAACGTGGTGAAGCCTTCTCTACAGGCACGCTGGCGAATGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7MTP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.25

100

0.813
  pilU Pseudomonas stutzeri DSM 10701

58.757

96.196

0.565
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Acinetobacter baylyi ADP1

41.86

93.478

0.391
  pilT Acinetobacter nosocomialis M2

42.183

92.12

0.389
  pilT Acinetobacter baumannii D1279779

42.183

92.12

0.389
  pilT Acinetobacter baumannii strain A118

42.183

92.12

0.389
  pilT Legionella pneumophila strain Lp02

42.183

92.12

0.389
  pilT Legionella pneumophila strain ERS1305867

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Pseudomonas stutzeri DSM 10701

40.936

92.935

0.38
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375

Multiple sequence alignment