Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACOI2P_RS15345 Genome accession   NZ_CP184784
Coordinates   3382873..3383985 (-) Length   370 a.a.
NCBI ID   WP_101055493.1    Uniprot ID   -
Organism   Shewanella chilikensis strain NTT9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3377873..3388985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOI2P_RS15325 (ACOI2P_15325) - 3378999..3379733 (-) 735 WP_226564522.1 tryptophan 2,3-dioxygenase family protein -
  ACOI2P_RS15330 (ACOI2P_15330) hemH 3379905..3380918 (-) 1014 WP_335923330.1 ferrochelatase -
  ACOI2P_RS15335 (ACOI2P_15335) - 3380953..3381444 (-) 492 WP_286996502.1 glutathione peroxidase -
  ACOI2P_RS15340 (ACOI2P_15340) - 3381607..3382716 (-) 1110 WP_420787375.1 hypothetical protein -
  ACOI2P_RS15345 (ACOI2P_15345) pilU 3382873..3383985 (-) 1113 WP_101055493.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOI2P_RS15350 (ACOI2P_15350) pilT 3383995..3385032 (-) 1038 WP_025009461.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOI2P_RS15355 (ACOI2P_15355) - 3385069..3385764 (+) 696 WP_165565434.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACOI2P_RS15360 (ACOI2P_15360) proC 3385897..3386715 (+) 819 WP_109248608.1 pyrroline-5-carboxylate reductase -
  ACOI2P_RS15365 (ACOI2P_15365) - 3386739..3387290 (+) 552 WP_101055490.1 YggT family protein -
  ACOI2P_RS15370 (ACOI2P_15370) yggU 3387290..3387577 (+) 288 WP_037436422.1 DUF167 family protein YggU -
  ACOI2P_RS15375 (ACOI2P_15375) - 3387640..3388074 (+) 435 WP_101055484.1 DUF4426 domain-containing protein -
  ACOI2P_RS15380 (ACOI2P_15380) rdgB 3388295..3388897 (+) 603 WP_101055482.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41069.17 Da        Isoelectric Point: 6.3711

>NTDB_id=1111238 ACOI2P_RS15345 WP_101055493.1 3382873..3383985(-) (pilU) [Shewanella chilikensis strain NTT9]
MDVRPFLKAMVERKASDLFITAAFPPSAKVDGELRPLAENAFTPAQSLEFVESLMTPEQKKEFHETRECNFAVGAKELGR
FRVSAFWQRESAGCVMRRIETKIPTVEQLYLPPILKDLVMSKRGLVIMVGGTGTGKSTSLAALIGYRNANARGHILTIED
PVEFVHQHGKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKVDGTGRRAAIEVLINTPRVASLIQKNELHVLKETMAKSNEQGMQTFDQA
LFKLYCEGEISYADALHHADSPNDLRLMIKLHSNDTGNSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1111238 ACOI2P_RS15345 WP_101055493.1 3382873..3383985(-) (pilU) [Shewanella chilikensis strain NTT9]
ATGGACGTCAGACCTTTTCTCAAGGCTATGGTGGAGCGCAAGGCCTCGGATCTCTTTATTACCGCCGCCTTTCCTCCCAG
TGCCAAGGTGGACGGTGAACTCAGGCCGTTGGCGGAAAACGCCTTTACCCCGGCGCAGTCGCTGGAGTTTGTCGAATCTT
TGATGACGCCGGAGCAGAAAAAGGAGTTTCACGAGACCCGTGAATGTAACTTTGCCGTGGGCGCCAAGGAGCTGGGGCGT
TTCCGGGTCAGTGCCTTCTGGCAACGGGAGTCGGCAGGCTGCGTGATGCGGCGCATCGAAACCAAGATCCCTACAGTGGA
GCAGTTGTACCTGCCGCCCATTCTCAAAGACTTGGTGATGAGCAAGCGCGGATTGGTGATCATGGTCGGGGGAACAGGTA
CCGGTAAGTCCACCTCGCTGGCGGCACTTATCGGTTATCGCAATGCCAATGCCAGGGGACATATACTCACCATCGAAGAT
CCGGTGGAATTTGTTCACCAACATGGCAAGAGCATTATCACCCAGCGGGAAGTGGGTATAGATACCGAATCTTTTGATGC
AGCGCTCAAGAGCTCATTGCGCCAGGCGCCGGATGTTATCCTTATCGGTGAGATCCGTACCCAGGAAACCATGGAGTTTG
CTCTGTCGTTCGCTGAAACCGGCCATTTGTGTATGGCAACTCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATGCACCTGGTGCCTGAGAGTAAGCACCAACAGCTGTTGTTTGACCTGTCGCTTAACCTGCGCGGTATTGTGGCCCAGCA
GTTGGTGCCCAAGGTGGACGGCACAGGTCGGCGCGCCGCCATCGAAGTGCTTATCAACACTCCCAGAGTGGCCAGTCTTA
TTCAAAAGAACGAGCTGCACGTGCTCAAGGAGACCATGGCCAAGTCCAACGAGCAGGGGATGCAAACCTTCGATCAGGCG
CTGTTCAAGCTCTACTGTGAAGGGGAGATCAGCTACGCCGATGCACTGCATCATGCTGACTCGCCTAACGACCTGCGACT
CATGATTAAACTCCACAGCAATGACACCGGCAACTCAGGTTTCATGGAGGGAGTCACCCTGGATATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.995

100

0.622
  pilU Vibrio cholerae strain A1552

58.378

100

0.584
  pilU Acinetobacter baylyi ADP1

59.943

95.135

0.57
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.58

93.243

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Legionella pneumophila strain Lp02

41.994

89.459

0.376
  pilT Legionella pneumophila strain ERS1305867

41.994

89.459

0.376
  pilT Acinetobacter baumannii D1279779

40.643

92.432

0.376
  pilT Acinetobacter baumannii strain A118

40.643

92.432

0.376
  pilT Acinetobacter nosocomialis M2

40.643

92.432

0.376
  pilT Acinetobacter baylyi ADP1

39.474

92.432

0.365
  pilT Pseudomonas stutzeri DSM 10701

38.841

93.243

0.362
  pilT Pseudomonas aeruginosa PAK

38.841

93.243

0.362

Multiple sequence alignment