Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACOBWA_RS01705 Genome accession   NZ_CP184640
Coordinates   360035..360472 (+) Length   145 a.a.
NCBI ID   WP_420230049.1    Uniprot ID   -
Organism   Psychrobacter sp. ER1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 355035..365472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOBWA_RS01680 (ACOBWA_01680) - 355081..356676 (+) 1596 WP_420230044.1 hypothetical protein -
  ACOBWA_RS01685 (ACOBWA_01685) - 356825..358453 (+) 1629 WP_420230045.1 PilC/PilY family type IV pilus protein -
  ACOBWA_RS01690 (ACOBWA_01690) - 358474..358644 (+) 171 WP_420230046.1 hypothetical protein -
  ACOBWA_RS01695 (ACOBWA_01695) - 358646..359131 (+) 486 WP_420230047.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACOBWA_RS01700 (ACOBWA_01700) - 359190..359669 (+) 480 WP_420230048.1 type IV pilin protein -
  ACOBWA_RS01705 (ACOBWA_01705) pilE 360035..360472 (+) 438 WP_420230049.1 pilin Machinery gene
  ACOBWA_RS01710 (ACOBWA_01710) - 360562..362322 (+) 1761 WP_420230050.1 Wzy polymerase domain-containing protein -
  ACOBWA_RS01715 (ACOBWA_01715) - 362385..362861 (+) 477 WP_420230051.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACOBWA_RS01720 (ACOBWA_01720) fdxA 362940..363263 (-) 324 WP_101205516.1 ferredoxin FdxA -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14891.96 Da        Isoelectric Point: 8.4829

>NTDB_id=1110390 ACOBWA_RS01705 WP_420230049.1 360035..360472(+) (pilE) [Psychrobacter sp. ER1]
MNTAQKGFTLIELMIVIAIIGILAAIALPAYQDYTKRARVGEALSLAGGAKTAVTEFYSSNNSWPSDNAAAGLPSASAIN
GNTVSSVTVASGKISVIVNDKVEKTPANAVLVLSPSASGGSVTWNCTSDAGTKLDNKFLPSECRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1110390 ACOBWA_RS01705 WP_420230049.1 360035..360472(+) (pilE) [Psychrobacter sp. ER1]
ATGAACACTGCACAAAAAGGTTTCACACTAATTGAACTAATGATCGTTATCGCTATTATCGGTATCTTGGCTGCGATCGC
TTTACCTGCTTATCAAGACTATACTAAGCGTGCTCGTGTTGGTGAAGCTTTAAGTCTAGCCGGTGGTGCTAAAACTGCTG
TTACAGAGTTCTATTCATCTAACAATAGTTGGCCTAGTGACAATGCGGCAGCTGGTCTACCTTCTGCTAGTGCGATTAAT
GGCAATACAGTTAGCAGTGTCACCGTAGCAAGTGGTAAAATTTCTGTGATAGTTAATGATAAAGTAGAAAAAACACCAGC
CAATGCTGTTCTTGTATTATCCCCATCTGCTAGTGGTGGAAGCGTAACTTGGAACTGTACATCTGATGCTGGTACTAAAT
TGGATAATAAGTTTTTACCATCAGAATGTCGTAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

50.633

100

0.552
  pilE Neisseria gonorrhoeae MS11

47.799

100

0.524
  comP Acinetobacter baylyi ADP1

47.17

100

0.517
  pilA2 Legionella pneumophila str. Paris

48.936

97.241

0.476
  pilA2 Legionella pneumophila strain ERS1305867

48.227

97.241

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

40.123

100

0.448
  pilA/pilA1 Eikenella corrodens VA1

38.854

100

0.421
  pilA Vibrio campbellii strain DS40M4

40.136

100

0.407
  pilA Acinetobacter baumannii strain A118

38.926

100

0.4
  pilA Pseudomonas aeruginosa PAK

36.184

100

0.379
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.762

98.621

0.372
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.324

97.931

0.366
  pilA Haemophilus influenzae 86-028NP

41.085

88.966

0.366
  pilA Haemophilus influenzae Rd KW20

41.085

88.966

0.366

Multiple sequence alignment