Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACLKM6_RS10750 Genome accession   NZ_CP183890
Coordinates   2239951..2241069 (+) Length   372 a.a.
NCBI ID   WP_005179770.1    Uniprot ID   N9M802
Organism   Acinetobacter indicus strain D11_NDM_tet(X5)     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2234951..2246069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM6_RS10735 - 2236933..2238030 (+) 1098 WP_104489467.1 acyltransferase family protein -
  ACLKM6_RS10740 - 2238076..2238762 (-) 687 WP_411686865.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLKM6_RS10745 pilT 2238886..2239923 (+) 1038 WP_005179768.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLKM6_RS10750 pilU 2239951..2241069 (+) 1119 WP_005179770.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLKM6_RS10755 fur 2241144..2241581 (-) 438 WP_005179771.1 ferric iron uptake transcriptional regulator -
  ACLKM6_RS10760 - 2241694..2242092 (+) 399 WP_005179773.1 outer membrane protein assembly factor BamE -
  ACLKM6_RS10765 - 2242135..2242458 (-) 324 WP_005179774.1 RnfH family protein -
  ACLKM6_RS10770 - 2242458..2243540 (-) 1083 WP_411686866.1 hypothetical protein -
  ACLKM6_RS10775 - 2243707..2244162 (-) 456 WP_005179777.1 bacteriohemerythrin -
  ACLKM6_RS10780 - 2244420..2245268 (-) 849 WP_411686873.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41757.97 Da        Isoelectric Point: 6.6668

>NTDB_id=1106898 ACLKM6_RS10750 WP_005179770.1 2239951..2241069(+) (pilU) [Acinetobacter indicus strain D11_NDM_tet(X5)]
MDFNDLLNLMIQQKASDLFITADVEPSMKINGQIVPVAKSKLTGEIVGQLLNSIMTDKQRKEFADTRECNFAITNRDKSA
RFRVSAFQQRDEPGMVLRRIETKIPTMEELKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHGQLFMDLSLNLKAIVAQQLIPTIDGNSRRAAIELLINSPLVSDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGERLMHATSNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1106898 ACLKM6_RS10750 WP_005179770.1 2239951..2241069(+) (pilU) [Acinetobacter indicus strain D11_NDM_tet(X5)]
ATGGACTTTAATGATTTATTGAATTTAATGATTCAGCAAAAAGCATCGGACCTGTTTATTACGGCCGATGTTGAGCCTTC
CATGAAAATTAACGGCCAGATTGTGCCGGTGGCCAAATCCAAACTGACCGGGGAAATTGTTGGCCAGCTGCTCAACTCGA
TTATGACCGACAAGCAGCGCAAGGAATTTGCTGACACCCGTGAATGTAACTTTGCCATCACCAACCGTGATAAAAGCGCG
CGTTTCCGTGTCAGCGCTTTTCAGCAGCGTGATGAACCGGGCATGGTGCTGCGTCGGATTGAAACCAAAATTCCGACCAT
GGAAGAACTGAAACTGCCACCGATTTTAAAAGATCTGGCCATGACCAAACGTGGCATCATCATCTTTGTCGGGGCAACCG
GTACCGGTAAGTCGACCTCTTTGGCGTCGATTATTGGTTATCGTAACCAGAATTCCAAAGGTCATATCATCACCATTGAA
GACCCGATTGAATTTGTGCACGAACACGCCGGCTGTATTATTACCCAGCGTGAAGTAGGCATTGATACCGATTCGTTTGA
AGTGGCACTGAAAAACACCTTGCGTCAGGCCCCAGATGTGATCCTGATTGGTGAGATCCGTTCACGTGAAACCATGGATT
ATGCAATTGCCTTTGCCGAAACCGGTCACCTGGTATTTGCCACCCTGCACGCCAACAACGCCAACCAAGCCATTGACCGG
ATTATCCACTTCTTTGAGGCTGACCGTCATGGCCAGCTGTTTATGGATTTATCACTGAACCTGAAAGCCATTGTGGCACA
GCAGCTGATTCCTACTATTGATGGCAACTCACGTCGTGCCGCGATTGAACTGCTGATTAACTCGCCACTGGTGTCCGATT
TAATCCGTAAAGGCGATGTGCACGAAATTAAAGACCTGATGAAGCGTTCACGTGAGCTGGGTATGCAAACCTTTGACCAG
GCCCTGTATGACCTGTATAAAGCCAAGCAGATCAGCTACAAAGATGCCCTGAAACATGCCGACTCGCCAAACGATTTGCG
TCTGCAAATCAAGCTGTCGGAAGAAGGCGGCGAGCGTTTAATGCATGCCACGTCAAATATTACCTTCGACGGACAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9M802

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

68.464

99.731

0.683
  pilU Vibrio cholerae strain A1552

57.55

94.355

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395
  pilT Legionella pneumophila strain Lp02

41.42

90.86

0.376
  pilT Legionella pneumophila strain ERS1305867

41.42

90.86

0.376
  pilT Pseudomonas aeruginosa PAK

40.462

93.011

0.376
  pilT Acinetobacter baumannii D1279779

40.525

92.204

0.374
  pilT Acinetobacter nosocomialis M2

40.525

92.204

0.374
  pilT Acinetobacter baumannii strain A118

40.525

92.204

0.374
  pilT Acinetobacter baylyi ADP1

39.942

92.204

0.368
  pilT Neisseria gonorrhoeae MS11

40.294

91.398

0.368
  pilT Neisseria meningitidis 8013

40.294

91.398

0.368
  pilT Pseudomonas stutzeri DSM 10701

39.017

93.011

0.363

Multiple sequence alignment