Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACN0NO_RS14825 Genome accession   NZ_CP183298
Coordinates   3230460..3230867 (-) Length   135 a.a.
NCBI ID   WP_095363635.1    Uniprot ID   -
Organism   Stenotrophomonas rhizophila strain SJ-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3210350..3239910 3230460..3230867 within 0


Gene organization within MGE regions


Location: 3210350..3239910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN0NO_RS14770 (ACN0NO_14770) - 3210350..3210898 (+) 549 WP_419403255.1 SymE family type I addiction module toxin -
  ACN0NO_RS14775 (ACN0NO_14775) - 3211022..3211504 (-) 483 WP_419403256.1 hypothetical protein -
  ACN0NO_RS14780 (ACN0NO_14780) - 3211712..3212074 (-) 363 WP_419403257.1 hypothetical protein -
  ACN0NO_RS14785 (ACN0NO_14785) - 3212270..3212962 (-) 693 WP_419403258.1 hypothetical protein -
  ACN0NO_RS14790 (ACN0NO_14790) - 3213664..3213843 (-) 180 WP_419403259.1 hypothetical protein -
  ACN0NO_RS14795 (ACN0NO_14795) - 3214162..3219000 (-) 4839 WP_419403260.1 RHS repeat-associated core domain-containing protein -
  ACN0NO_RS14800 (ACN0NO_14800) - 3219401..3219844 (-) 444 WP_419403261.1 hypothetical protein -
  ACN0NO_RS14805 (ACN0NO_14805) - 3219867..3224531 (-) 4665 WP_419403262.1 RHS repeat-associated core domain-containing protein -
  ACN0NO_RS14810 (ACN0NO_14810) - 3224849..3227896 (-) 3048 WP_419403263.1 spermine synthase -
  ACN0NO_RS14815 (ACN0NO_14815) pilB 3228172..3229899 (-) 1728 WP_095363637.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACN0NO_RS14820 (ACN0NO_14820) pilA 3229953..3230360 (-) 408 WP_419403264.1 pilin Machinery gene
  ACN0NO_RS14825 (ACN0NO_14825) pilA 3230460..3230867 (-) 408 WP_095363635.1 pilin Machinery gene
  ACN0NO_RS14830 (ACN0NO_14830) pilC 3231224..3232480 (+) 1257 WP_419403265.1 type II secretion system F family protein Machinery gene
  ACN0NO_RS14835 (ACN0NO_14835) pilD 3232487..3233350 (+) 864 WP_068853817.1 prepilin peptidase Machinery gene
  ACN0NO_RS14840 (ACN0NO_14840) coaE 3233358..3233966 (+) 609 WP_419403266.1 dephospho-CoA kinase -
  ACN0NO_RS14845 (ACN0NO_14845) - 3234056..3235420 (-) 1365 WP_095363631.1 sensor histidine kinase -
  ACN0NO_RS14850 (ACN0NO_14850) - 3235383..3236060 (-) 678 WP_017356179.1 response regulator transcription factor -
  ACN0NO_RS14855 (ACN0NO_14855) - 3236068..3236538 (-) 471 WP_095363630.1 hypothetical protein -
  ACN0NO_RS14860 (ACN0NO_14860) rimK 3236771..3237676 (-) 906 WP_249833010.1 30S ribosomal protein S6--L-glutamate ligase -
  ACN0NO_RS14865 (ACN0NO_14865) - 3237895..3238674 (+) 780 WP_095363628.1 histidine phosphatase family protein -
  ACN0NO_RS14870 (ACN0NO_14870) - 3238676..3239548 (+) 873 WP_093533699.1 NAD(P)H-hydrate dehydratase -
  ACN0NO_RS14875 (ACN0NO_14875) - 3239551..3239910 (-) 360 WP_210130949.1 nuclear transport factor 2 family protein -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 14228.53 Da        Isoelectric Point: 8.4764

>NTDB_id=1105155 ACN0NO_RS14825 WP_095363635.1 3230460..3230867(-) (pilA) [Stenotrophomonas rhizophila strain SJ-3]
MKKQQGFTLIELMIVVAIIAILAAIALPMYQDYVAKSQVTAGLAEVTPGKTQYEVALNEGKTTVTGISDLGLKNSDRCVI
TPVTALSASGTLVCTLSGSPKIATKKVTLTRSDDGTWACTTDVDDKYRPSGCKKG

Nucleotide


Download         Length: 408 bp        

>NTDB_id=1105155 ACN0NO_RS14825 WP_095363635.1 3230460..3230867(-) (pilA) [Stenotrophomonas rhizophila strain SJ-3]
ATGAAGAAGCAGCAGGGCTTTACCCTCATCGAACTGATGATCGTCGTCGCGATCATCGCCATCCTGGCCGCCATCGCTCT
GCCGATGTACCAGGACTACGTTGCCAAGTCGCAGGTGACCGCTGGCCTGGCCGAAGTGACCCCGGGCAAGACCCAGTACG
AAGTTGCTCTGAACGAAGGCAAGACCACCGTTACCGGCATCAGCGACCTGGGTCTGAAGAATTCGGACCGCTGCGTGATC
ACGCCGGTCACGGCCCTGAGCGCCTCCGGCACCCTGGTCTGCACCCTGTCGGGCAGCCCGAAGATCGCCACCAAGAAGGT
CACCCTGACCCGTTCGGATGATGGCACCTGGGCATGCACCACCGACGTGGACGACAAGTACCGTCCGAGCGGCTGCAAGA
AGGGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-133)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

52.857

100

0.548
  pilA Pseudomonas aeruginosa PAK

46

100

0.511
  pilA/pilAI Pseudomonas stutzeri DSM 10701

51.111

100

0.511
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.704

100

0.437
  pilA Vibrio cholerae C6706

40.278

100

0.43
  pilA Vibrio cholerae strain A1552

40.278

100

0.43
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.278

100

0.43
  comP Acinetobacter baylyi ADP1

38.776

100

0.422
  pilE Neisseria gonorrhoeae MS11

34.356

100

0.415
  pilA2 Legionella pneumophila strain ERS1305867

37.762

100

0.4
  pilA2 Legionella pneumophila str. Paris

37.762

100

0.4
  pilA Vibrio parahaemolyticus RIMD 2210633

42.276

91.111

0.385

Multiple sequence alignment