Detailed information NTG1105153

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ACN0NO_RS14815
• Genome accession: NZ_CP183298
• Coordinates: 3228172..3229899 (-)
• Length: 575 a.a.
• NCBI ID: WP_095363637.1
• Uniprot ID: -
• Organism: Stenotrophomonas rhizophila strain SJ-3
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3210350..3239910	3228172..3229899	within	0

Gene organization within MGE regions

Location: 3210350..3239910

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACN0NO_RS14770 (ACN0NO_14770)	-	3210350..3210898 (+)	549	WP_419403255.1	SymE family type I addiction module toxin	-
ACN0NO_RS14775 (ACN0NO_14775)	-	3211022..3211504 (-)	483	WP_419403256.1	hypothetical protein	-
ACN0NO_RS14780 (ACN0NO_14780)	-	3211712..3212074 (-)	363	WP_419403257.1	hypothetical protein	-
ACN0NO_RS14785 (ACN0NO_14785)	-	3212270..3212962 (-)	693	WP_419403258.1	hypothetical protein	-
ACN0NO_RS14790 (ACN0NO_14790)	-	3213664..3213843 (-)	180	WP_419403259.1	hypothetical protein	-
ACN0NO_RS14795 (ACN0NO_14795)	-	3214162..3219000 (-)	4839	WP_419403260.1	RHS repeat-associated core domain-containing protein	-
ACN0NO_RS14800 (ACN0NO_14800)	-	3219401..3219844 (-)	444	WP_419403261.1	hypothetical protein	-
ACN0NO_RS14805 (ACN0NO_14805)	-	3219867..3224531 (-)	4665	WP_419403262.1	RHS repeat-associated core domain-containing protein	-
ACN0NO_RS14810 (ACN0NO_14810)	-	3224849..3227896 (-)	3048	WP_419403263.1	spermine synthase	-
ACN0NO_RS14815 (ACN0NO_14815)	pilB	3228172..3229899 (-)	1728	WP_095363637.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACN0NO_RS14820 (ACN0NO_14820)	pilA	3229953..3230360 (-)	408	WP_419403264.1	pilin	Machinery gene
ACN0NO_RS14825 (ACN0NO_14825)	pilA	3230460..3230867 (-)	408	WP_095363635.1	pilin	Machinery gene
ACN0NO_RS14830 (ACN0NO_14830)	pilC	3231224..3232480 (+)	1257	WP_419403265.1	type II secretion system F family protein	Machinery gene
ACN0NO_RS14835 (ACN0NO_14835)	pilD	3232487..3233350 (+)	864	WP_068853817.1	prepilin peptidase	Machinery gene
ACN0NO_RS14840 (ACN0NO_14840)	coaE	3233358..3233966 (+)	609	WP_419403266.1	dephospho-CoA kinase	-
ACN0NO_RS14845 (ACN0NO_14845)	-	3234056..3235420 (-)	1365	WP_095363631.1	sensor histidine kinase	-
ACN0NO_RS14850 (ACN0NO_14850)	-	3235383..3236060 (-)	678	WP_017356179.1	response regulator transcription factor	-
ACN0NO_RS14855 (ACN0NO_14855)	-	3236068..3236538 (-)	471	WP_095363630.1	hypothetical protein	-
ACN0NO_RS14860 (ACN0NO_14860)	rimK	3236771..3237676 (-)	906	WP_249833010.1	30S ribosomal protein S6--L-glutamate ligase	-
ACN0NO_RS14865 (ACN0NO_14865)	-	3237895..3238674 (+)	780	WP_095363628.1	histidine phosphatase family protein	-
ACN0NO_RS14870 (ACN0NO_14870)	-	3238676..3239548 (+)	873	WP_093533699.1	NAD(P)H-hydrate dehydratase	-
ACN0NO_RS14875 (ACN0NO_14875)	-	3239551..3239910 (-)	360	WP_210130949.1	nuclear transport factor 2 family protein	-

Sequence

Protein

• Length: 575 a.a.
• Molecular weight: 62159.19 Da
• Isoelectric Point: 6.0477

>NTDB_id=1105153 ACN0NO_RS14815 WP_095363637.1 3228172..3229899(-) (pilB) [Stenotrophomonas rhizophila strain SJ-3]
MNAVATANLVGITGIARRLVQDGALEEASARDAMAKAAAAKQPLPQYFADKKLVTSAQLAAANAVEFGMPLLDVSVFDSA
QNAISLVSEELLRKHHVLPLFKRGGKLFIGTSDPTHSLDEIKFHTNLVVEPILVDEEQIRRTLEQWQSKHDTLGGGLGGD
DEGMGDLDVSAGDEDGGTSDTGHDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRFRIDGLLKNVAKAPVKL
NQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEEVQQKLFL
EAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPFNITSSVTLVIAQRLARRLCNNCKRPVDLPPNA
LLAEGFTQAQLDAGITLYEAVGCEECTEGYKGRTGIYQVMPMSEEISVIVLAGGNAIQIAEAAQRAGVNDLRQSALVKAA
AGTTSLAEINRVTKD

Nucleotide

• Length: 1728 bp

>NTDB_id=1105153 ACN0NO_RS14815 WP_095363637.1 3228172..3229899(-) (pilB) [Stenotrophomonas rhizophila strain SJ-3]
ATGAACGCCGTTGCTACTGCCAATCTCGTCGGCATAACCGGCATTGCCCGCCGTCTGGTTCAAGACGGGGCCCTTGAGGA
AGCGTCAGCGCGGGACGCGATGGCCAAGGCCGCAGCGGCCAAACAGCCGCTCCCGCAGTATTTCGCGGACAAGAAGCTCG
TCACTTCGGCGCAATTGGCGGCTGCCAACGCCGTCGAATTCGGCATGCCGCTGTTGGATGTGTCGGTGTTCGACAGTGCG
CAGAATGCGATCAGCCTGGTCAGCGAGGAACTGCTGCGCAAGCATCACGTGCTTCCGCTGTTCAAGCGTGGCGGAAAGTT
GTTCATTGGTACCAGTGATCCGACCCACTCGTTGGACGAGATCAAGTTCCATACGAATCTGGTGGTCGAGCCGATCCTGG
TGGACGAGGAGCAGATCCGTCGCACCTTGGAGCAATGGCAGTCCAAGCATGACACCCTGGGTGGCGGTCTCGGCGGCGAC
GACGAGGGGATGGGGGATCTCGACGTTTCAGCTGGTGATGAAGATGGCGGAACAAGTGATACCGGCCACGATGCCAAAGG
CGATGACACGCCTGTGGTCAAGTTCGTCAACAAGGTACTGGTAGACGCGATCCGCAAAGGTGCTTCGGACATCCACTTCG
AGCCGTACGAGGATGACTATCGAGTGCGCTTCCGCATCGATGGCCTGCTCAAGAATGTTGCCAAGGCGCCGGTCAAGCTC
AACCAGCGCATTGCCGCGCGCCTGAAGGTCATGTCGCAGCTGGACATCGCCGAAAAACGCGTTCCGCAGGACGGGCGCAT
CAAGCTCAACCTTTCAAAGACAAAGCAGATCGACTTCCGCGTCAGCACCTTGCCCACGCTGTTTGGCGAGAAGATCGTGC
TGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTTGGGTACGAGGAAGTGCAGCAGAAGCTTTTTCTG
GAGGCCATCCACAAGCCGTATGGCATGGTGCTTGTTACCGGTCCTACCGGCTCAGGCAAGACCGTGTCCCTGTACACCGC
GCTTGGCATCCTCAACGACGAAACGCGCAACATCTCCACGGCGGAAGATCCTGTCGAAATCCGCTTGCCAGGTGTCAACC
AGGTGCAGCAGAACGTCAAGCGCGGCATGACATTTGCCGCAGCGTTGCGTTCGTTCCTGCGTCAGGATCCTGACATCATC
ATGGTGGGCGAAATTCGTGACCTGGAAACGGCCGAGATCGCGATCAAGGCTGCCCAGACAGGCCACATGGTGTTGTCGAC
CCTGCACACCAATGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATTGCGCCCTTCAACATCACAAGTTCCG
TAACCCTTGTGATTGCGCAGCGCCTGGCTCGCCGGCTGTGCAACAATTGCAAGCGGCCGGTGGATCTGCCGCCGAACGCG
TTGCTTGCCGAGGGCTTTACCCAGGCCCAGCTGGACGCCGGGATTACCTTGTATGAGGCCGTTGGCTGCGAAGAGTGCAC
CGAGGGTTACAAGGGTCGTACCGGTATCTACCAGGTCATGCCAATGAGTGAGGAGATCTCTGTGATCGTCCTGGCGGGGG
GTAATGCCATCCAGATCGCTGAAGCCGCCCAGCGTGCGGGTGTGAACGATCTGAGGCAGTCTGCATTGGTCAAGGCAGCG
GCAGGTACTACCAGCTTGGCCGAGATCAACCGCGTGACCAAGGACTAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (51-150)

• Type II/IV secretion system protein (T2SSE) (198-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	55.517	100	0.56
pilB	Acinetobacter baylyi ADP1	56.261	98.609	0.555
pilB	Legionella pneumophila strain ERS1305867	53.345	98.783	0.527
pilF	Neisseria gonorrhoeae MS11	49.912	98.609	0.492
pilB	Vibrio cholerae strain A1552	49.174	94.783	0.466
pilB	Vibrio parahaemolyticus RIMD 2210633	46.996	98.435	0.463
pilB	Vibrio campbellii strain DS40M4	45.754	100	0.459