Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ACM66N_RS07280 Genome accession   NZ_CP182111
Coordinates   1538918..1539331 (-) Length   137 a.a.
NCBI ID   WP_312230289.1    Uniprot ID   -
Organism   Pseudomonas sp. OHS18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1533918..1544331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM66N_RS07265 (ACM66N_07265) tfpZ 1536442..1537149 (-) 708 WP_416638433.1 TfpX/TfpZ family type IV pilin accessory protein -
  ACM66N_RS07270 (ACM66N_07270) - 1537476..1537961 (-) 486 WP_416638435.1 hypothetical protein -
  ACM66N_RS07275 (ACM66N_07275) - 1537931..1538707 (-) 777 WP_416638436.1 hypothetical protein -
  ACM66N_RS07280 (ACM66N_07280) pilA/pilAI 1538918..1539331 (-) 414 WP_312230289.1 pilin Machinery gene
  ACM66N_RS07285 (ACM66N_07285) pilB 1539594..1541297 (+) 1704 WP_312230290.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACM66N_RS07290 (ACM66N_07290) pilC 1541300..1542523 (+) 1224 WP_288497825.1 type II secretion system F family protein Machinery gene
  ACM66N_RS07295 (ACM66N_07295) pilD 1542513..1543400 (+) 888 WP_416638437.1 prepilin peptidase Machinery gene
  ACM66N_RS07300 (ACM66N_07300) coaE 1543517..1544125 (+) 609 WP_312230292.1 dephospho-CoA kinase -
  ACM66N_RS07305 (ACM66N_07305) yacG 1544122..1544322 (+) 201 WP_312230293.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14180.22 Da        Isoelectric Point: 8.4733

>NTDB_id=1100404 ACM66N_RS07280 WP_312230289.1 1538918..1539331(-) (pilA/pilAI) [Pseudomonas sp. OHS18]
MKAQKGFTLIELMIVVAIIGILAAVAIPAYQDYTVRSNAAAALAEVTPGKVGFEQAINEGNAPSLTATDKGYIGITSSTS
YCTVTLIAANGGIQCATKGGNQTNFNGKNIQLTRDVNTGIWGCTSSLDAKYKPGKCS

Nucleotide


Download         Length: 414 bp        

>NTDB_id=1100404 ACM66N_RS07280 WP_312230289.1 1538918..1539331(-) (pilA/pilAI) [Pseudomonas sp. OHS18]
ATGAAGGCCCAAAAAGGCTTTACCCTGATCGAACTGATGATCGTTGTTGCCATCATCGGTATCCTTGCTGCTGTTGCTAT
CCCTGCTTACCAAGATTACACCGTCCGTTCTAACGCTGCCGCTGCGTTGGCTGAAGTTACTCCTGGTAAAGTAGGTTTTG
AGCAGGCAATTAATGAAGGAAACGCTCCGTCGTTGACTGCGACTGATAAAGGCTATATTGGCATTACCTCTTCCACGTCT
TATTGCACCGTGACCTTGATTGCAGCTAATGGCGGTATTCAGTGCGCAACAAAAGGCGGTAATCAAACCAATTTCAACGG
TAAGAATATTCAGCTGACGCGTGATGTAAACACCGGCATCTGGGGCTGCACCAGCAGCCTCGACGCTAAATACAAGCCAG
GCAAATGTTCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

75

100

0.766
  pilA/pilAII Pseudomonas stutzeri DSM 10701

64.662

97.08

0.628
  pilA Pseudomonas aeruginosa PAK

43.624

100

0.474
  comP Acinetobacter baylyi ADP1

41.497

100

0.445
  pilA Acinetobacter baumannii strain A118

43.262

100

0.445
  pilA2 Legionella pneumophila str. Paris

41.912

99.27

0.416
  pilA2 Legionella pneumophila strain ERS1305867

41.912

99.27

0.416
  pilA Vibrio cholerae C6706

38.621

100

0.409
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.621

100

0.409
  pilA Vibrio cholerae strain A1552

38.621

100

0.409
  pilA Vibrio parahaemolyticus RIMD 2210633

38.406

100

0.387

Multiple sequence alignment