Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACM66N_RS07290 Genome accession   NZ_CP182111
Coordinates   1541300..1542523 (+) Length   407 a.a.
NCBI ID   WP_288497825.1    Uniprot ID   -
Organism   Pseudomonas sp. OHS18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1536300..1547523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM66N_RS07265 (ACM66N_07265) tfpZ 1536442..1537149 (-) 708 WP_416638433.1 TfpX/TfpZ family type IV pilin accessory protein -
  ACM66N_RS07270 (ACM66N_07270) - 1537476..1537961 (-) 486 WP_416638435.1 hypothetical protein -
  ACM66N_RS07275 (ACM66N_07275) - 1537931..1538707 (-) 777 WP_416638436.1 hypothetical protein -
  ACM66N_RS07280 (ACM66N_07280) pilA/pilAI 1538918..1539331 (-) 414 WP_312230289.1 pilin Machinery gene
  ACM66N_RS07285 (ACM66N_07285) pilB 1539594..1541297 (+) 1704 WP_312230290.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACM66N_RS07290 (ACM66N_07290) pilC 1541300..1542523 (+) 1224 WP_288497825.1 type II secretion system F family protein Machinery gene
  ACM66N_RS07295 (ACM66N_07295) pilD 1542513..1543400 (+) 888 WP_416638437.1 prepilin peptidase Machinery gene
  ACM66N_RS07300 (ACM66N_07300) coaE 1543517..1544125 (+) 609 WP_312230292.1 dephospho-CoA kinase -
  ACM66N_RS07305 (ACM66N_07305) yacG 1544122..1544322 (+) 201 WP_312230293.1 DNA gyrase inhibitor YacG -
  ACM66N_RS07310 (ACM66N_07310) - 1544361..1544888 (+) 528 WP_312230294.1 MOSC domain-containing protein -
  ACM66N_RS07315 (ACM66N_07315) - 1545134..1546432 (+) 1299 WP_312230295.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 43648.25 Da        Isoelectric Point: 9.9751

>NTDB_id=1100406 ACM66N_RS07290 WP_288497825.1 1541300..1542523(+) (pilC) [Pseudomonas sp. OHS18]
MAAAAKAPKTSTFVWEGKDRKGSIVKGELSGQNPALVKAQLRKQGINPTKVRKKGISLGGAGKKVKPLDIALFARQMATM
MKAGVPLLQSFDIIADGVEKPSMRKLVDELKQHVAAGNSFAAALRTKPEYFDDLFCNLVDAGEQSGALETLLDRVATYKE
KTEALKAKIRKAMNYPIAVVVVAVIVTCILLIKVVPQFQEVFAGFGAELPAFTLFVIGISEALQKWWFIVLIGFVGAGYA
FLQAKQRSEKFRDTLDRAALKAPIVGNIVYKASVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRNAVNKVKQDVTS
GMQLNFSMRSTNAFPAMAVQMTAIGEESGALDSMLDKVASYYEAEVDNAVDGLTALMEPLIMSVLGVLVGGLIIAMYLPI
FQLGGVV

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=1100406 ACM66N_RS07290 WP_288497825.1 1541300..1542523(+) (pilC) [Pseudomonas sp. OHS18]
ATGGCAGCAGCAGCAAAAGCGCCCAAGACCAGCACCTTCGTTTGGGAAGGCAAAGATCGCAAGGGCAGCATCGTCAAGGG
CGAGCTCAGCGGCCAGAACCCGGCGCTGGTCAAGGCGCAGTTGCGCAAGCAAGGCATCAACCCGACCAAGGTGCGCAAGA
AAGGCATCAGTCTAGGCGGCGCGGGTAAAAAGGTTAAGCCACTAGATATTGCCTTGTTTGCGCGCCAAATGGCGACCATG
ATGAAAGCGGGTGTACCGCTGCTGCAGTCTTTCGACATTATTGCTGACGGTGTTGAAAAGCCCAGCATGCGCAAGCTGGT
GGATGAACTAAAGCAGCACGTAGCGGCGGGCAATAGTTTTGCTGCAGCATTACGCACCAAACCTGAATACTTTGATGATC
TTTTCTGCAACTTGGTAGACGCTGGTGAGCAGTCGGGAGCTCTGGAAACGCTACTGGATCGCGTCGCTACTTATAAGGAA
AAAACAGAGGCGCTCAAAGCAAAAATTAGAAAAGCGATGAATTATCCGATTGCGGTTGTAGTGGTGGCCGTAATCGTTAC
ATGCATTCTACTGATCAAGGTAGTGCCTCAGTTCCAAGAGGTCTTCGCCGGTTTCGGCGCTGAACTTCCTGCTTTCACAT
TGTTTGTCATTGGGATATCTGAGGCATTACAGAAGTGGTGGTTTATTGTACTGATAGGTTTCGTAGGTGCGGGGTATGCA
TTTCTACAAGCCAAGCAACGGTCTGAAAAGTTCAGAGATACTCTAGACCGAGCAGCACTGAAAGCGCCGATCGTAGGTAA
TATTGTCTACAAGGCCTCTGTCGCAAGGTATGCGAGAACTCTTTCGACAACTTTTGCTGCCGGCGTACCCTTGGTGGAAG
CACTGGACTCGGTTTCAGGTGCGACAGGTAATGTGGTGTTCCGTAACGCCGTCAACAAGGTGAAGCAGGACGTTACCAGC
GGCATGCAGTTAAACTTCTCTATGCGCTCAACGAATGCATTTCCAGCCATGGCGGTTCAGATGACCGCGATCGGGGAGGA
GTCCGGCGCCCTCGACAGCATGTTAGACAAGGTGGCTAGCTACTATGAGGCGGAAGTAGACAATGCCGTGGATGGCCTTA
CGGCTCTAATGGAACCACTAATCATGTCTGTCCTCGGCGTATTGGTTGGCGGCCTGATCATTGCCATGTACCTGCCGATC
TTCCAGCTGGGTGGTGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(73-196)

Type II secretion system (T2SS), protein F (T2SSF)

(278-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.95

99.263

0.794
  pilC Acinetobacter baylyi ADP1

61.765

100

0.619
  pilC Acinetobacter baumannii D1279779

61.916

100

0.619
  pilC Legionella pneumophila strain ERS1305867

51.861

99.017

0.514
  pilG Neisseria gonorrhoeae MS11

44.03

98.771

0.435
  pilG Neisseria meningitidis 44/76-A

44.03

98.771

0.435
  pilC Vibrio cholerae strain A1552

42.211

97.789

0.413
  pilC Vibrio campbellii strain DS40M4

40.547

98.771

0.4
  pilC Thermus thermophilus HB27

37.685

99.754

0.376

Multiple sequence alignment