Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACM66N_RS07295 Genome accession   NZ_CP182111
Coordinates   1542513..1543400 (+) Length   295 a.a.
NCBI ID   WP_416638437.1    Uniprot ID   -
Organism   Pseudomonas sp. OHS18     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1537513..1548400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM66N_RS07275 (ACM66N_07275) - 1537931..1538707 (-) 777 WP_416638436.1 hypothetical protein -
  ACM66N_RS07280 (ACM66N_07280) pilA/pilAI 1538918..1539331 (-) 414 WP_312230289.1 pilin Machinery gene
  ACM66N_RS07285 (ACM66N_07285) pilB 1539594..1541297 (+) 1704 WP_312230290.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACM66N_RS07290 (ACM66N_07290) pilC 1541300..1542523 (+) 1224 WP_288497825.1 type II secretion system F family protein Machinery gene
  ACM66N_RS07295 (ACM66N_07295) pilD 1542513..1543400 (+) 888 WP_416638437.1 prepilin peptidase Machinery gene
  ACM66N_RS07300 (ACM66N_07300) coaE 1543517..1544125 (+) 609 WP_312230292.1 dephospho-CoA kinase -
  ACM66N_RS07305 (ACM66N_07305) yacG 1544122..1544322 (+) 201 WP_312230293.1 DNA gyrase inhibitor YacG -
  ACM66N_RS07310 (ACM66N_07310) - 1544361..1544888 (+) 528 WP_312230294.1 MOSC domain-containing protein -
  ACM66N_RS07315 (ACM66N_07315) - 1545134..1546432 (+) 1299 WP_312230295.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 295 a.a.        Molecular weight: 32521.74 Da        Isoelectric Point: 7.9137

>NTDB_id=1100407 ACM66N_RS07295 WP_416638437.1 1542513..1543400(+) (pilD) [Pseudomonas sp. OHS18]
MWFNQCPFLRLLAGNTPAFVFCTLIIGLLVGSFLNVVIHRLPKMMLRDWRIQAREVLELPAEPAGETYNLVLPHSSCPHC
NHEIRPWENIPVISYLFLRGKCAGCKAKISLRYPLVELACGLISAYVASHFGLGWQAAGMLVLAWGLLAMSLIDADHQLL
PDALVLPLLWLGLIANNFGLFTSLEDALWGAVAGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILL
SSVVGAVLGMIMLRLRDASTSTPIPFGPYLAIAGFIALLWGKQITEGYLRFAGFN

Nucleotide


Download         Length: 888 bp        

>NTDB_id=1100407 ACM66N_RS07295 WP_416638437.1 1542513..1543400(+) (pilD) [Pseudomonas sp. OHS18]
GTGTGGTTTAATCAATGTCCGTTTTTGAGGCTCCTGGCCGGCAATACGCCGGCCTTTGTTTTCTGCACCCTGATCATCGG
CCTACTGGTCGGCAGCTTTCTCAACGTAGTCATTCACCGCTTGCCAAAGATGATGCTGCGCGACTGGCGCATCCAAGCGC
GCGAAGTACTGGAGCTGCCTGCCGAGCCGGCCGGAGAAACCTATAACCTGGTACTGCCTCACTCCAGCTGCCCACACTGC
AACCATGAAATCCGACCGTGGGAAAATATCCCAGTCATCAGCTATCTCTTCTTGCGCGGCAAGTGCGCCGGCTGCAAGGC
GAAGATCAGTCTGCGCTATCCACTGGTCGAACTCGCCTGCGGCCTGATCTCAGCCTACGTCGCTTCGCACTTCGGGCTTG
GCTGGCAAGCGGCAGGAATGTTGGTGCTGGCCTGGGGCCTCCTGGCGATGAGCCTGATCGATGCCGATCACCAATTGCTG
CCGGACGCCCTGGTGCTGCCGCTGCTGTGGCTGGGCTTGATCGCCAACAATTTTGGGCTGTTTACCAGCCTGGAAGACGC
CTTGTGGGGCGCCGTCGCGGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGTA
TGGGTTATGGCGACTTCAAATTGCTGGCCATGCTTGGTGCCTGGGGCGGCTGGCAGATTCTGCCGCTGACCATTTTGCTG
TCTTCGGTAGTCGGTGCCGTGCTCGGGATGATCATGCTGCGCTTACGCGATGCCAGCACCAGTACGCCGATTCCCTTTGG
CCCCTACCTGGCCATCGCGGGCTTTATCGCGCTGCTGTGGGGCAAGCAGATTACCGAAGGGTATTTGCGCTTCGCGGGAT
TCAATTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(25-131)

Type IV leader peptidase family (Peptidase_A24)

(141-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

54.212

92.542

0.502
  pilD Vibrio cholerae strain A1552

55.056

90.508

0.498
  pilD Acinetobacter baumannii D1279779

51.625

93.898

0.485
  pilD Acinetobacter nosocomialis M2

50.903

93.898

0.478
  pilD Neisseria gonorrhoeae MS11

49.618

88.814

0.441

Multiple sequence alignment