Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACM66N_RS07285 Genome accession   NZ_CP182111
Coordinates   1539594..1541297 (+) Length   567 a.a.
NCBI ID   WP_312230290.1    Uniprot ID   -
Organism   Pseudomonas sp. OHS18     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1534594..1546297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM66N_RS07265 (ACM66N_07265) tfpZ 1536442..1537149 (-) 708 WP_416638433.1 TfpX/TfpZ family type IV pilin accessory protein -
  ACM66N_RS07270 (ACM66N_07270) - 1537476..1537961 (-) 486 WP_416638435.1 hypothetical protein -
  ACM66N_RS07275 (ACM66N_07275) - 1537931..1538707 (-) 777 WP_416638436.1 hypothetical protein -
  ACM66N_RS07280 (ACM66N_07280) pilA/pilAI 1538918..1539331 (-) 414 WP_312230289.1 pilin Machinery gene
  ACM66N_RS07285 (ACM66N_07285) pilB 1539594..1541297 (+) 1704 WP_312230290.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACM66N_RS07290 (ACM66N_07290) pilC 1541300..1542523 (+) 1224 WP_288497825.1 type II secretion system F family protein Machinery gene
  ACM66N_RS07295 (ACM66N_07295) pilD 1542513..1543400 (+) 888 WP_416638437.1 prepilin peptidase Machinery gene
  ACM66N_RS07300 (ACM66N_07300) coaE 1543517..1544125 (+) 609 WP_312230292.1 dephospho-CoA kinase -
  ACM66N_RS07305 (ACM66N_07305) yacG 1544122..1544322 (+) 201 WP_312230293.1 DNA gyrase inhibitor YacG -
  ACM66N_RS07310 (ACM66N_07310) - 1544361..1544888 (+) 528 WP_312230294.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62412.22 Da        Isoelectric Point: 5.7781

>NTDB_id=1100405 ACM66N_RS07285 WP_312230290.1 1539594..1541297(+) (pilB) [Pseudomonas sp. OHS18]
MSENIPLSGLAKQVVLAELLDEKAAQQAQVQAKRSKLSLVHYLAQNKLVKSRALAELTADQFGIAYFDLSALDKESQPKD
LVSEKLVRQHRALPLWRRGNKLFVGISDPSNHQVVTDIQFSTGLSTEALLVEDDKLGDAIEKFFDTGHTGLEDMGDVDLD
GLETESVDDDNKGEGGGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYERTYRVRFRTDGILHETARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKISKSKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEESQKELYLNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPKQGMDFTQALRAFLRQDPDIIMVGEIRD
LDTASIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCSSCKKEVDVPREALLEEGFP
ESKIGSFKIYGPVGCENCKNGYKGRVGIYEVVKNTPSLQRIIMEEGNSIDIAAQMRKDGFNDLRTSALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1100405 ACM66N_RS07285 WP_312230290.1 1539594..1541297(+) (pilB) [Pseudomonas sp. OHS18]
ATGAGCGAGAACATTCCCCTTTCCGGCCTGGCCAAGCAAGTGGTACTGGCTGAGCTACTGGACGAGAAGGCCGCTCAACA
GGCGCAGGTACAAGCCAAGCGTAGCAAGCTCTCTCTAGTTCATTACCTCGCCCAGAACAAGCTGGTAAAAAGCCGTGCAT
TGGCCGAGCTCACCGCGGATCAGTTCGGCATCGCCTATTTCGATCTAAGCGCGCTGGACAAAGAGAGTCAGCCGAAGGAT
TTGGTCAGCGAAAAGCTCGTGCGGCAGCACCGTGCCCTGCCACTATGGCGGCGTGGTAACAAGCTGTTCGTCGGCATATC
CGACCCTTCCAATCACCAAGTCGTTACCGATATTCAATTCAGCACAGGGCTCTCCACAGAAGCTTTGCTGGTCGAGGACG
ACAAACTGGGCGATGCCATCGAAAAGTTTTTCGATACTGGCCATACCGGCCTAGAAGACATGGGCGACGTTGATCTCGAT
GGCCTGGAAACCGAATCGGTCGATGACGATAATAAGGGCGAGGGTGGCGGCCAGGATGCCGATGACGCGCCTGTGGTGCG
TTTCGTGAACAAGATGCTGCTCGATGCCATCAAGGGTGGCTCCTCGGACCTGCACTTCGAGCCCTATGAGCGCACCTACC
GCGTACGCTTTCGTACCGACGGTATCCTGCACGAGACGGCGCGTCCGCCGATTCAGCTGGCACCGCGCATTTCAGCACGA
CTAAAGGTCATGGCCGGGCTGGATATCTCCGAGCGGCGCAAGCCGCAGGACGGCCGCATCAAGATGAAGATTTCCAAGAG
CAAGTCCATCGACTTTCGCGTCAACACTTTGCCAACACTATGGGGCGAGAAGATCGTGATGCGGATACTCGACTCTTCAA
GTGCGCAAATGGGTATCGATGCCCTGGGCTACGAGGAGAGCCAGAAGGAGCTGTATCTCAATGCCCTCAAACAGCCCCAG
GGCATGATCCTGGTCACCGGCCCCACCGGTTCGGGCAAAACCGTATCGCTCTATACAGGCCTCAATATTCTCAATACGCC
CGACGTGAATATCTCCACCGCTGAAGACCCGGTAGAGATCAACCTTGAAGGCATCAATCAGGTCAACGTCAACCCCAAGC
AAGGCATGGATTTCACACAGGCCCTGCGCGCCTTCCTGCGTCAGGACCCGGATATCATCATGGTCGGCGAAATCCGCGAC
CTGGATACCGCATCCATTGCCATCAAGGCCGCACAAACCGGCCACATGGTGATGTCGACCCTACACACCAACAGCGCCGC
GGAAACACTGACCCGCCTGCGCAATATGGGTGTACCCTCCTTCAACATCGCCACCTCGGTCAACCTGATCATCGCCCAGC
GTCTGGCGCGTAAGCTGTGTTCCTCCTGCAAGAAGGAGGTGGATGTTCCCCGCGAGGCACTGCTCGAAGAAGGTTTCCCG
GAGAGCAAGATCGGCAGCTTCAAGATCTATGGCCCGGTGGGCTGTGAAAATTGCAAGAACGGTTACAAAGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGAGCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGATATTGCCGCGC
AAATGCGCAAAGACGGCTTTAACGACCTGCGCACCTCGGCCCTTGTGAAGGCCATGCAAGGCGTGACCAGCCTTGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.436

100

0.594
  pilB Acinetobacter baylyi ADP1

57.624

99.471

0.573
  pilB Legionella pneumophila strain ERS1305867

55.07

100

0.556
  pilB Vibrio parahaemolyticus RIMD 2210633

51.296

95.238

0.489
  pilB Vibrio cholerae strain A1552

50

97.002

0.485
  pilB Vibrio campbellii strain DS40M4

49.634

96.296

0.478
  pilF Neisseria gonorrhoeae MS11

47.957

99.295

0.476
  pilB/pilB1 Synechocystis sp. PCC 6803

36.495

100

0.4
  pilF Thermus thermophilus HB27

36.972

100

0.37

Multiple sequence alignment