Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABXS88_RS12545 Genome accession   NZ_AP031572
Coordinates   2777161..2778294 (+) Length   377 a.a.
NCBI ID   WP_353672383.1    Uniprot ID   -
Organism   Synechocystis sp. LKSZ1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2772161..2783294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXS88_RS12530 (OLK001_24620) - 2772637..2773572 (+) 936 WP_353672380.1 isoaspartyl peptidase/L-asparaginase -
  ABXS88_RS12535 (OLK001_24630) - 2773662..2774927 (+) 1266 WP_353672381.1 hypothetical protein -
  ABXS88_RS12540 (OLK001_24640) pilB/pilB1 2775046..2777061 (+) 2016 WP_353672382.1 GspE/PulE family protein Machinery gene
  ABXS88_RS12545 (OLK001_24650) pilT 2777161..2778294 (+) 1134 WP_353672383.1 type IV pilus twitching motility protein PilT Machinery gene
  ABXS88_RS12550 (OLK001_24660) - 2778336..2779553 (+) 1218 WP_353672384.1 type II secretion system F family protein -
  ABXS88_RS12555 (OLK001_24670) - 2779550..2780260 (-) 711 WP_353672385.1 class I SAM-dependent methyltransferase -
  ABXS88_RS12560 (OLK001_24680) - 2780405..2781721 (+) 1317 WP_353672386.1 deoxyguanosinetriphosphate triphosphohydrolase -
  ABXS88_RS12565 (OLK001_24690) - 2781789..2782382 (+) 594 WP_353672387.1 rhomboid family intramembrane serine protease -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41149.55 Da        Isoelectric Point: 7.3261

>NTDB_id=110036 ABXS88_RS12545 WP_353672383.1 2777161..2778294(+) (pilT) [Synechocystis sp. LKSZ1]
MAVEYIIEDLMEQLVEMGGSDMHIQAGAPVYFRISGKLNPINDEVLSPQDSQKLIFSMLNNSQRKELEQSWELDCSYGVK
GLARFRVNVYKERGCYAACLRALSSKIPNFEQLGLPTIIREMAERPRGLILVTGQTGSGKTTTLAAILDLINRTRAEHIL
TIEDPIEYVFPNVKSLFHQRQKGEDTKSFANALRAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTNSAAST
IDRILDVFPANQQAQIRAMLSNSLLAVFSQCLVKKKAPKPGEFGRAMAQEIMVITPAIANLIREGKAPQIYSAIQTGAKL
GMQTMEQALATLVMAGTISMEEGLSKSGKPDELQRLIGGAVPSPNAPAANAAAKVRR

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=110036 ABXS88_RS12545 WP_353672383.1 2777161..2778294(+) (pilT) [Synechocystis sp. LKSZ1]
ATGGCTGTGGAATATATTATTGAAGACCTGATGGAACAATTGGTGGAAATGGGCGGCTCGGATATGCATATCCAGGCTGG
CGCTCCCGTCTATTTCCGCATTAGTGGCAAATTGAACCCCATCAATGATGAAGTTTTGAGCCCCCAGGATAGCCAGAAGC
TGATTTTTAGTATGCTGAACAATTCCCAGCGCAAGGAATTGGAGCAGAGTTGGGAATTAGATTGTTCCTACGGCGTTAAG
GGCCTGGCCCGCTTTCGGGTCAATGTGTATAAAGAACGCGGCTGTTACGCGGCCTGTTTACGGGCCCTGTCGTCGAAGAT
TCCGAATTTTGAACAGTTGGGTCTGCCGACCATTATTCGGGAAATGGCGGAACGACCGAGGGGTCTAATCCTGGTGACGG
GACAAACGGGCTCAGGTAAAACCACCACCTTGGCGGCAATTTTGGATTTAATCAACCGGACGCGGGCGGAGCATATTCTC
ACTATTGAAGACCCGATTGAATACGTTTTCCCTAACGTCAAGAGTCTCTTCCACCAGCGTCAGAAAGGGGAAGACACCAA
GAGCTTCGCAAATGCTTTACGAGCGGCCCTGCGGGAAGACCCGGATATTATTCTGGTGGGGGAAATGCGGGACTTGGAAA
CCATTTCCCTCGCCATTAGTGCGGCAGAAACCGGACACTTGGTCTTTGGTACCCTCCATACCAATTCAGCCGCCAGTACT
ATCGACCGGATTTTGGACGTCTTTCCCGCTAACCAGCAGGCCCAAATTCGAGCCATGCTCTCCAATTCTCTACTAGCGGT
GTTCAGCCAATGTCTAGTTAAGAAAAAGGCCCCAAAACCGGGGGAATTTGGCCGAGCCATGGCCCAAGAAATTATGGTGA
TTACCCCCGCTATTGCCAACCTGATCCGGGAAGGGAAAGCCCCTCAGATTTACTCCGCCATCCAAACCGGGGCTAAATTA
GGGATGCAGACCATGGAACAGGCCCTAGCAACCTTGGTGATGGCCGGCACCATTTCCATGGAAGAGGGCCTATCCAAGAG
TGGTAAGCCCGATGAGCTACAGCGCTTAATCGGCGGGGCCGTCCCCTCCCCGAATGCTCCCGCCGCTAATGCCGCGGCCA
AAGTCCGGCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(96-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

54.203

91.512

0.496
  pilT Legionella pneumophila strain Lp02

54.203

91.512

0.496
  pilT Pseudomonas aeruginosa PAK

52.161

92.042

0.48
  pilT Acinetobacter baumannii D1279779

51.585

92.042

0.475
  pilT Acinetobacter baumannii strain A118

51.585

92.042

0.475
  pilT Acinetobacter nosocomialis M2

51.585

92.042

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.37

97.613

0.472
  pilT Acinetobacter baylyi ADP1

51.297

92.042

0.472
  pilT Pseudomonas stutzeri DSM 10701

51.009

92.042

0.469
  pilT Vibrio cholerae strain A1552

51.613

90.451

0.467
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.613

90.451

0.467
  pilT Neisseria meningitidis 8013

50.149

88.859

0.446
  pilT Neisseria gonorrhoeae MS11

49.851

88.859

0.443

Multiple sequence alignment