Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   ABXS88_RS12540 Genome accession   NZ_AP031572
Coordinates   2775046..2777061 (+) Length   671 a.a.
NCBI ID   WP_353672382.1    Uniprot ID   -
Organism   Synechocystis sp. LKSZ1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2770046..2782061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXS88_RS12525 (OLK001_24610) - 2771019..2772248 (-) 1230 WP_353672379.1 pentapeptide repeat-containing protein -
  ABXS88_RS12530 (OLK001_24620) - 2772637..2773572 (+) 936 WP_353672380.1 isoaspartyl peptidase/L-asparaginase -
  ABXS88_RS12535 (OLK001_24630) - 2773662..2774927 (+) 1266 WP_353672381.1 hypothetical protein -
  ABXS88_RS12540 (OLK001_24640) pilB/pilB1 2775046..2777061 (+) 2016 WP_353672382.1 GspE/PulE family protein Machinery gene
  ABXS88_RS12545 (OLK001_24650) pilT 2777161..2778294 (+) 1134 WP_353672383.1 type IV pilus twitching motility protein PilT Machinery gene
  ABXS88_RS12550 (OLK001_24660) - 2778336..2779553 (+) 1218 WP_353672384.1 type II secretion system F family protein -
  ABXS88_RS12555 (OLK001_24670) - 2779550..2780260 (-) 711 WP_353672385.1 class I SAM-dependent methyltransferase -
  ABXS88_RS12560 (OLK001_24680) - 2780405..2781721 (+) 1317 WP_353672386.1 deoxyguanosinetriphosphate triphosphohydrolase -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 75172.87 Da        Isoelectric Point: 5.1318

>NTDB_id=110035 ABXS88_RS12540 WP_353672382.1 2775046..2777061(+) (pilB/pilB1) [Synechocystis sp. LKSZ1]
MAYSSSKKTRAVALRNYFSPFGNKLVAGGHIEPEQLRQALVQVKKSGRSLPEEIQAITGRALSPDLLRQYKKNQLFELKV
LYGVDSLDPELAPVATREMAELIQDLIPLDTCRRYKVLPMARQDTDPPSILVVMVNPDDLAAQDELQRILRFKGLELRRL
VITQEDFEQLLEQYYGIQQELEQVREKQAKDKELDKLADITDIVGSLDMNLGEVRDEDADSLDSKDANQAPVINLVNKVM
AKALQEGASDIHIEPQEETLRIRFRKDGVLHQAFDPLPRKVTPAVVARFKIMADLDIAERRAPQDGKIRRIYQGRKVDFR
VNTIPSRYGEKVCLRILDNTATQLGLDKLITNEETLNTVRELASRPYGLILVTGPTGSGKSTSLYSVLAERNSPEVNINT
AEDPIEYSLPGITQVQVIREKGMDFANILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARL
DEMGVEPFMVSGALLGVLAQRLMRRVCTECRIPYNPGREELSRFGFSISGDESITFYQANSLSPDEIHEARKTGTLCPKC
NGSGYKGRVGVYEVMRNSERIASLINEGATTDRIKDCAVEEGMVTLLAYSLNLVQEGYTTLEEVERVTFTDTGLESELRA
KRKSALTCRTCQAELQQEWLDCPYCMTPRFT

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=110035 ABXS88_RS12540 WP_353672382.1 2775046..2777061(+) (pilB/pilB1) [Synechocystis sp. LKSZ1]
ATGGCTTATTCATCTTCTAAAAAGACTCGTGCAGTTGCACTGCGTAACTACTTTTCTCCCTTTGGCAATAAGCTGGTGGC
AGGGGGACATATTGAACCCGAACAGCTACGCCAGGCCCTGGTGCAAGTCAAGAAGTCAGGGCGGTCTTTACCCGAAGAGA
TTCAAGCCATTACCGGTCGGGCCCTGTCGCCGGATCTCCTCCGCCAGTACAAGAAAAACCAACTGTTTGAGCTAAAAGTT
CTCTACGGGGTCGATAGTCTCGACCCGGAATTAGCCCCAGTGGCTACGCGGGAAATGGCGGAGCTGATTCAAGACCTTAT
TCCCCTAGATACCTGTCGGCGGTATAAGGTTCTTCCCATGGCTCGGCAGGATACCGACCCACCCAGCATCCTAGTGGTGA
TGGTCAACCCCGATGACCTGGCGGCCCAGGATGAACTCCAGCGCATTCTGCGTTTCAAAGGTCTGGAGCTACGGCGTTTG
GTGATCACCCAGGAAGATTTTGAACAACTCCTGGAGCAGTATTATGGCATTCAGCAAGAACTCGAGCAGGTTCGCGAAAA
ACAAGCGAAGGACAAGGAATTAGATAAATTAGCCGACATTACCGATATTGTTGGCTCCCTGGACATGAATTTAGGGGAGG
TGCGGGATGAAGATGCCGATTCCCTGGACTCTAAGGACGCGAACCAAGCTCCAGTCATTAATTTGGTCAATAAAGTAATG
GCCAAGGCCCTCCAGGAAGGGGCCTCGGATATTCATATTGAACCCCAGGAAGAAACCCTCCGCATTCGCTTCCGCAAGGA
TGGCGTTTTGCACCAGGCCTTTGATCCCCTACCCCGCAAGGTGACCCCCGCAGTAGTTGCTCGTTTCAAGATCATGGCAG
ATTTGGACATTGCGGAGCGACGGGCCCCCCAAGATGGCAAAATTCGACGCATCTACCAGGGCCGGAAGGTCGATTTTCGA
GTTAACACCATCCCAAGTCGCTACGGGGAAAAGGTTTGTCTGCGGATTCTGGACAATACAGCCACCCAATTGGGTCTCGA
TAAACTGATTACCAATGAAGAAACGCTAAATACCGTGCGAGAACTAGCCAGTCGTCCCTACGGCTTAATTTTGGTGACAG
GGCCAACGGGGTCAGGAAAATCCACCAGTTTGTACTCTGTCCTGGCGGAGCGCAATAGCCCTGAAGTCAATATCAATACG
GCAGAGGATCCCATTGAGTACTCCTTACCTGGTATTACCCAGGTACAAGTCATTCGGGAAAAGGGAATGGACTTTGCCAA
TATCCTGCGGGCCTTTATGCGTCAGGATCCCGATGTGATTCTAGTCGGGGAAACCCGCGATAAGGAAACGGCCAAAACCG
CTATCGAGGCGGCCTTGACTGGTCACTTAGTATTGACAACCCTTCATACCAATGATGCGGCCGGGGCCATTGCCCGCCTA
GATGAAATGGGGGTGGAACCCTTCATGGTGTCCGGGGCTCTGCTGGGAGTCTTGGCCCAGCGCTTGATGCGGCGGGTCTG
TACCGAATGCCGGATTCCCTATAATCCCGGCCGTGAAGAACTTTCGCGCTTTGGCTTCTCTATTTCTGGAGATGAATCCA
TTACCTTCTACCAGGCCAATAGCCTCAGTCCTGATGAAATTCATGAGGCCCGCAAAACAGGGACGCTTTGTCCTAAATGC
AATGGCAGTGGCTACAAAGGCCGAGTTGGTGTGTACGAAGTGATGCGGAACTCGGAACGCATTGCCAGCCTGATTAATGA
AGGTGCCACCACCGACCGGATTAAGGATTGTGCCGTGGAGGAAGGGATGGTTACGCTTTTAGCCTACAGCTTAAATCTGG
TACAGGAAGGCTATACGACCCTTGAAGAAGTAGAGCGCGTCACCTTCACCGATACCGGACTAGAGTCGGAATTACGAGCC
AAGCGCAAGAGCGCCCTCACCTGCCGGACTTGTCAAGCGGAACTACAGCAAGAATGGCTCGATTGTCCCTACTGTATGAC
TCCCCGCTTTACTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(238-505)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

84.743

98.659

0.836

Multiple sequence alignment