Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACNFWM_RS18020 Genome accession   NZ_CP181388
Coordinates   3817726..3818862 (+) Length   378 a.a.
NCBI ID   WP_132648919.1    Uniprot ID   A0A4V2SGA3
Organism   Rubrivivax gelatinosus isolate YF10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3812726..3823862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNFWM_RS17990 - 3813324..3813824 (+) 501 WP_132648929.1 Lrp/AsnC ligand binding domain-containing protein -
  ACNFWM_RS17995 - 3813992..3814201 (+) 210 WP_132648927.1 hypothetical protein -
  ACNFWM_RS18000 - 3814300..3815250 (+) 951 WP_416461459.1 IS481 family transposase -
  ACNFWM_RS18005 - 3815262..3815954 (-) 693 WP_132648925.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACNFWM_RS18010 pilT 3815997..3817040 (+) 1044 WP_132648923.1 type IV pilus twitching motility protein PilT Machinery gene
  ACNFWM_RS18015 - 3817184..3817705 (+) 522 WP_132648921.1 cyclic nucleotide-binding domain-containing protein -
  ACNFWM_RS18020 pilU 3817726..3818862 (+) 1137 WP_132648919.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNFWM_RS18025 - 3818919..3819809 (+) 891 WP_132648917.1 NAD(P)-dependent oxidoreductase -
  ACNFWM_RS18030 - 3819837..3820469 (-) 633 WP_132649009.1 BON domain-containing protein -
  ACNFWM_RS18035 - 3820484..3821041 (-) 558 WP_132648915.1 SIS domain-containing protein -
  ACNFWM_RS18040 - 3821093..3821479 (-) 387 WP_132648913.1 YraN family protein -
  ACNFWM_RS18045 rsmI 3821472..3822371 (+) 900 WP_132648911.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42065.36 Da        Isoelectric Point: 6.9139

>NTDB_id=1099959 ACNFWM_RS18020 WP_132648919.1 3817726..3818862(+) (pilU) [Rubrivivax gelatinosus isolate YF10]
MERDQASKFVNDLLRLMVTRSGSDLFLTADFPPAIKVDGKVTKVSPQPLTGQHTLQLARAVMNDKQAAEFERTKECNFAI
SPLGVGRFRVNAFMQQGQVGLVLRTIPQTLPTIDSLGLPGVLKEIAMTKRGLVIFVGATGSGKSTSLAAMVDWRNEHSYG
HIITVEDPIEFVHPHKNCIVTQREVGVDTDGWEQALKNTLRQAPDVILMGEIRDRETMEHAVAFSETGHLCMATLHANSA
NQALDRIINFFPEERRGQLLMDLSLNLKALVSQRLLPREQGRGRIAAVEIMLNTPLVSDMIFKGEIGEIKELMKRSRELG
MQTFDQSLFDLYEAGAVGYEDALRNADSVNDLRLQIKLNSQRARSRDLAAGTENMRVI

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1099959 ACNFWM_RS18020 WP_132648919.1 3817726..3818862(+) (pilU) [Rubrivivax gelatinosus isolate YF10]
ATGGAACGCGACCAGGCCTCGAAATTCGTCAACGACCTGCTGCGGCTGATGGTCACGCGCAGCGGCTCGGACCTCTTTCT
CACCGCCGACTTCCCGCCGGCGATCAAGGTCGACGGCAAGGTGACCAAGGTCTCGCCGCAGCCGCTGACCGGCCAGCACA
CGCTGCAGCTGGCGCGCGCGGTGATGAACGACAAGCAGGCCGCCGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
AGCCCGCTGGGCGTGGGGCGCTTTCGCGTCAACGCCTTCATGCAGCAGGGCCAGGTCGGGCTGGTGCTGCGCACGATCCC
GCAGACGCTGCCGACGATCGACTCGCTGGGTCTGCCCGGCGTGCTCAAGGAGATCGCGATGACCAAGCGCGGGCTGGTCA
TCTTCGTCGGCGCCACCGGCTCGGGCAAGAGCACCTCGCTGGCGGCGATGGTCGACTGGCGCAACGAACACTCCTACGGC
CACATCATCACCGTCGAGGACCCGATCGAGTTCGTGCATCCGCACAAGAACTGCATCGTCACGCAGCGCGAGGTGGGCGT
GGACACCGACGGCTGGGAGCAGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACGATGGAGCACGCGGTGGCGTTCTCCGAGACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAGGAGCGCCGCGGCCAGCTGCTGATGGACCTGTCGCTGAACCT
GAAGGCGCTGGTCAGCCAGCGCCTGCTGCCACGCGAGCAGGGGCGCGGCCGCATCGCGGCGGTGGAGATCATGCTCAACA
CGCCGCTGGTCTCGGACATGATCTTCAAGGGCGAGATCGGCGAGATCAAGGAGCTGATGAAACGCTCGCGCGAGCTCGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTCTACGAAGCCGGCGCCGTGGGCTACGAGGACGCGCTGCGCAACGCCGA
TTCGGTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCACGCAGCCGCGACCTCGCCGCCGGCACGGAGA
ACATGCGCGTCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V2SGA3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

59.218

94.709

0.561
  pilU Vibrio cholerae strain A1552

52.304

97.619

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

43.323

89.153

0.386
  pilT Acinetobacter nosocomialis M2

43.544

88.095

0.384
  pilT Acinetobacter baumannii D1279779

43.544

88.095

0.384
  pilT Acinetobacter baumannii strain A118

43.544

88.095

0.384
  pilT Acinetobacter baylyi ADP1

43.544

88.095

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Legionella pneumophila strain ERS1305867

42.943

88.095

0.378
  pilT Legionella pneumophila strain Lp02

42.943

88.095

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

87.831

0.37
  pilT Vibrio cholerae strain A1552

42.169

87.831

0.37

Multiple sequence alignment