Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACM67E_RS07430 Genome accession   NZ_CP181244
Coordinates   1585286..1586419 (+) Length   377 a.a.
NCBI ID   WP_416190831.1    Uniprot ID   -
Organism   Neisseria sp. CCUG12390     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1580286..1591419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM67E_RS07415 (ACM67E_07415) lepB 1581439..1582425 (-) 987 WP_416190828.1 signal peptidase I -
  ACM67E_RS07420 (ACM67E_07420) lepA 1582425..1584218 (-) 1794 WP_416190829.1 translation elongation factor 4 -
  ACM67E_RS07425 (ACM67E_07425) - 1584356..1585057 (-) 702 WP_416190830.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  ACM67E_RS07430 (ACM67E_07430) pilU 1585286..1586419 (+) 1134 WP_416190831.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACM67E_RS07435 (ACM67E_07435) - 1586626..1587198 (-) 573 WP_416190832.1 50S ribosomal protein L25/general stress protein Ctc -
  ACM67E_RS07440 (ACM67E_07440) - 1587266..1588255 (-) 990 WP_416190833.1 ribose-phosphate pyrophosphokinase -
  ACM67E_RS07460 (ACM67E_07460) ispE 1588668..1589546 (-) 879 WP_416190834.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
  ACM67E_RS07465 (ACM67E_07465) - 1589558..1590142 (-) 585 WP_416192753.1 outer membrane lipoprotein LolB -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42390.46 Da        Isoelectric Point: 6.4185

>NTDB_id=1099463 ACM67E_RS07430 WP_416190831.1 1585286..1586419(+) (pilU) [Neisseria sp. CCUG12390]
MFDNSNTPNAKDELFSWLRHMNKYKGSDLFITTNFPPAMKVDGKITRLSDTPLTAEKCMELAFSIMNTKQIEEFSSTNEC
NFAISLPDTSRFRVNAMVQRGATAMVFRAITSNIPKFESLNLPPVLKDIALKKRGLVIFVGGTGSGKSTSLASLIDYRNE
NTFDHIITIEDPIEFVHQHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLH
ANSTNQALDRIINFFPEERRTQLLTDLSLNLQAFISQRLVPREGGKGRVAAVEVLLNSPLIAELIHKGEIHSIKELMKKS
TAQGMQTFDQALYELYERGDVSFQDAIKNADSAHDLRLDIQLRSRRAQNAGPDLELI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1099463 ACM67E_RS07430 WP_416190831.1 1585286..1586419(+) (pilU) [Neisseria sp. CCUG12390]
ATGTTTGACAACTCGAATACCCCCAACGCCAAAGACGAATTGTTTTCTTGGCTGCGTCATATGAATAAATACAAAGGCTC
GGATTTATTCATCACTACCAATTTCCCGCCCGCCATGAAAGTGGACGGCAAAATCACCCGACTGAGCGATACGCCGCTGA
CCGCAGAAAAATGCATGGAATTGGCGTTTTCGATTATGAACACCAAACAAATCGAAGAATTTTCGTCGACCAACGAATGC
AACTTCGCCATCAGCCTACCGGATACCAGCCGCTTCCGTGTCAACGCCATGGTTCAGCGCGGTGCCACGGCTATGGTGTT
CCGCGCCATTACCAGCAACATTCCCAAATTCGAGAGTCTGAACCTGCCGCCGGTATTGAAAGACATCGCCCTGAAAAAAC
GCGGCCTGGTAATTTTCGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTGGCTTCGCTGATCGATTACCGCAACGAA
AACACGTTCGACCACATCATCACCATCGAAGACCCGATTGAATTCGTGCACCAACACAAAAACTGCATTATTACCCAACG
GGAAGTCGGTGTGGATACCGAAAACTGGATGGCCGCGCTGAAAAATACCCTGCGCCAGGCACCGGATGTGATTTTGATTG
GCGAAATCCGTGACCGCGAAACCATGGATTACGCGATTGCCTTTGCCGAAACCGGCCACTTGTGCATGGCTACGCTGCAC
GCCAACAGCACCAACCAGGCACTCGACCGCATCATCAACTTCTTCCCCGAAGAACGCCGTACGCAGCTTCTCACCGATTT
ATCGCTTAACCTTCAAGCATTTATCTCTCAACGCCTAGTTCCGCGTGAAGGCGGCAAAGGACGTGTCGCGGCAGTGGAAG
TATTGCTGAATTCACCGCTGATTGCAGAACTGATTCACAAAGGAGAAATTCACAGCATTAAGGAATTAATGAAAAAATCT
ACCGCACAAGGCATGCAGACCTTTGACCAAGCTCTGTACGAACTGTATGAGCGTGGTGATGTGAGTTTCCAAGATGCGAT
TAAAAATGCCGACTCTGCCCATGATTTGCGTTTGGACATCCAATTGCGCAGCCGCCGTGCGCAGAATGCCGGGCCGGATT
TGGAATTGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(45-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.648

93.369

0.576
  pilU Acinetobacter baylyi ADP1

58.017

90.981

0.528
  pilU Vibrio cholerae strain A1552

53.76

95.225

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.544

88.329

0.385
  pilT Acinetobacter baumannii D1279779

43.827

85.942

0.377
  pilT Acinetobacter baumannii strain A118

43.827

85.942

0.377
  pilT Legionella pneumophila strain Lp02

43.827

85.942

0.377
  pilT Legionella pneumophila strain ERS1305867

43.827

85.942

0.377
  pilT Acinetobacter nosocomialis M2

43.827

85.942

0.377
  pilT Acinetobacter baylyi ADP1

42.378

87.003

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.415

85.676

0.363
  pilT Vibrio cholerae strain A1552

42.415

85.676

0.363
  pilT Neisseria meningitidis 8013

40.597

88.859

0.361
  pilT Pseudomonas aeruginosa PAK

41.463

87.003

0.361

Multiple sequence alignment