Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACM2ZB_RS12425 Genome accession   NZ_CP180589
Coordinates   2646113..2647243 (+) Length   376 a.a.
NCBI ID   WP_313439047.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain ZSL2493     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2641113..2652243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM2ZB_RS12405 (ACM2ZB_12405) - 2642377..2643747 (-) 1371 WP_416055654.1 pyridoxal-phosphate dependent enzyme -
  ACM2ZB_RS12410 (ACM2ZB_12410) - 2644184..2644420 (-) 237 WP_313439038.1 YdcH family protein -
  ACM2ZB_RS12415 (ACM2ZB_12415) - 2644745..2645629 (-) 885 WP_416055655.1 hypothetical protein -
  ACM2ZB_RS12420 (ACM2ZB_12420) - 2645626..2645991 (-) 366 WP_313439044.1 DUF4398 domain-containing protein -
  ACM2ZB_RS12425 (ACM2ZB_12425) pilU 2646113..2647243 (+) 1131 WP_313439047.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACM2ZB_RS12430 (ACM2ZB_12430) - 2647303..2648586 (+) 1284 WP_416055656.1 putative DNA modification/repair radical SAM protein -
  ACM2ZB_RS12435 (ACM2ZB_12435) - 2648567..2650033 (+) 1467 WP_416055657.1 UdgX family uracil-DNA binding protein -
  ACM2ZB_RS12440 (ACM2ZB_12440) maiA 2650074..2650742 (-) 669 WP_416055658.1 maleylacetoacetate isomerase -
  ACM2ZB_RS12445 (ACM2ZB_12445) - 2650757..2651746 (-) 990 WP_416055659.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41587.87 Da        Isoelectric Point: 6.3738

>NTDB_id=1096857 ACM2ZB_RS12425 WP_313439047.1 2646113..2647243(+) (pilU) [Stenotrophomonas maltophilia strain ZSL2493]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALADAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKNGVIDQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSSATQPSITHGF

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1096857 ACM2ZB_RS12425 WP_313439047.1 2646113..2647243(+) (pilU) [Stenotrophomonas maltophilia strain ZSL2493]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCTTGACCACCGGTGCGCCGGTGTA
CATCAAGATCGAGGGCAAGCTGTACCCGCTGGGCAATACCGGCCTGCCGCCGGGCATGGTCAAGAAAATCGCCTACTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTGAACATGGCCATCGCCCTGGCCGACGCGGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGGGGCGAAGTCGGCATGGTCATCCGTGCCATCCGCAGCCGGATTCCCAGCATCGA
AGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGTGGGCTGGTGCTGGTGGTGGGGTCCACCGGCT
CGGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCATGCCTTCCACAA
CGCGCTGAAGAATGCGATGCGTGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCGAGACGATGGAGGCAG
CCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
TCTGGTGAAGAACAAGGAGGGCCGTCGCCTGCCGGCCACCGAGGTGCTGATCAACACCCCGATGATCCGCGACCTGCTGC
GTCGCGGCCAGGTGCACGAGATCAAGGCCGCGATGGAGGCCTCGCTGGAAGAAGGCATGGAAAGCTTCGACCAGTGCCTG
TTCCGCCTGGCCAAGAACGGGGTGATCGACCAGGAGGAAGCGCTGCGCGCCGCCGACTCGCGAGATGGCCTGGCCCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGTGGCGAGCACGATCCGTACGCGGATTTCTCCTCGGCCACCCAGCCCAGCATCACCC
ACGGGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.479

0.489
  pilU Acinetobacter baylyi ADP1

49.435

94.149

0.465
  pilU Vibrio cholerae strain A1552

49.153

94.149

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.241

100

0.449
  pilT Pseudomonas aeruginosa PAK

42.604

89.894

0.383
  pilT Acinetobacter baumannii D1279779

41.716

89.894

0.375
  pilT Acinetobacter nosocomialis M2

41.716

89.894

0.375
  pilT Acinetobacter baumannii strain A118

41.716

89.894

0.375
  pilT Acinetobacter baylyi ADP1

41.176

90.426

0.372
  pilT Pseudomonas stutzeri DSM 10701

41.124

89.894

0.37
  pilT Legionella pneumophila strain ERS1305867

40.828

89.894

0.367
  pilT Legionella pneumophila strain Lp02

40.828

89.894

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

88.564

0.364
  pilT Vibrio cholerae strain A1552

41.141

88.564

0.364

Multiple sequence alignment