Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ACL0K2_RS08800 Genome accession   NZ_CP178378
Coordinates   1961632..1962051 (+) Length   139 a.a.
NCBI ID   WP_255983138.1    Uniprot ID   -
Organism   Pseudomonas sp. YJ42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1956632..1967051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL0K2_RS08785 (ACL0K2_08785) pilD 1957614..1958480 (-) 867 WP_413044241.1 prepilin peptidase Machinery gene
  ACL0K2_RS08790 (ACL0K2_08790) pilC 1958483..1959700 (-) 1218 WP_413044242.1 type II secretion system F family protein Machinery gene
  ACL0K2_RS08795 (ACL0K2_08795) pilB 1959702..1961405 (-) 1704 WP_413044243.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACL0K2_RS08800 (ACL0K2_08800) pilA/pilAI 1961632..1962051 (+) 420 WP_255983138.1 pilin Machinery gene
  ACL0K2_RS08805 (ACL0K2_08805) pilA/pilAII 1962120..1962545 (+) 426 WP_413044244.1 pilin Regulator
  ACL0K2_RS08810 (ACL0K2_08810) - 1962571..1963962 (+) 1392 WP_413044245.1 O-antigen ligase family protein -
  ACL0K2_RS08815 (ACL0K2_08815) tfpZ 1963971..1964705 (+) 735 WP_413044246.1 TfpX/TfpZ family type IV pilin accessory protein -
  ACL0K2_RS08820 (ACL0K2_08820) - 1964988..1965125 (+) 138 WP_413044247.1 YtoQ family protein -
  ACL0K2_RS08825 (ACL0K2_08825) - 1965110..1965379 (-) 270 Protein_1733 TonB-dependent receptor plug domain-containing protein -
  ACL0K2_RS08830 (ACL0K2_08830) - 1965381..1965716 (+) 336 Protein_1734 YtoQ family protein -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14515.55 Da        Isoelectric Point: 5.8192

>NTDB_id=1089231 ACL0K2_RS08800 WP_255983138.1 1961632..1962051(+) (pilA/pilAI) [Pseudomonas sp. YJ42]
MKAQMQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRSNAAAALAEITPGKIGFEQAINEGKTPSLTSTDEGYIGITSS
TSYCTVALDTAADGHIQCTAKGGNAGKFDTKTITLNRTADGEWSCASTLDAKYKPGKCS

Nucleotide


Download         Length: 420 bp        

>NTDB_id=1089231 ACL0K2_RS08800 WP_255983138.1 1961632..1962051(+) (pilA/pilAI) [Pseudomonas sp. YJ42]
ATGAAAGCTCAGATGCAAAAAGGTTTTACTCTGATTGAATTGATGATCGTTGTGGCGATCATCGGCATTCTGGCTGCAAT
TGCGCTGCCGGCCTATCAGGACTACACCGTACGCTCCAACGCCGCCGCTGCCCTGGCCGAAATCACCCCCGGCAAGATCG
GCTTTGAGCAGGCGATCAACGAGGGTAAAACTCCGTCGCTTACTTCTACTGACGAAGGCTACATTGGTATCACCAGCTCG
ACTTCTTACTGTACCGTAGCGCTGGATACTGCTGCGGATGGTCACATCCAGTGTACTGCCAAAGGCGGCAATGCTGGCAA
GTTTGATACCAAAACAATTACGCTTAATCGTACCGCTGACGGCGAGTGGAGCTGCGCCAGTACCCTTGACGCCAAATACA
AGCCAGGCAAGTGTTCCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

96.403

100

0.964
  pilA/pilAII Pseudomonas stutzeri DSM 10701

67.424

94.964

0.64
  pilA Pseudomonas aeruginosa PAK

47.682

100

0.518
  comP Acinetobacter baylyi ADP1

44

100

0.475
  pilA Acinetobacter baumannii strain A118

47.445

98.561

0.468
  pilA2 Legionella pneumophila str. Paris

43.704

97.122

0.424
  pilA2 Legionella pneumophila strain ERS1305867

43.704

97.122

0.424
  pilA Vibrio cholerae strain A1552

39.86

100

0.41
  pilA Vibrio cholerae C6706

39.86

100

0.41
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.86

100

0.41

Multiple sequence alignment