Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACL0K2_RS08795 Genome accession   NZ_CP178378
Coordinates   1959702..1961405 (-) Length   567 a.a.
NCBI ID   WP_413044243.1    Uniprot ID   -
Organism   Pseudomonas sp. YJ42     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1954702..1966405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL0K2_RS08770 (ACL0K2_08770) - 1955686..1956378 (+) 693 WP_045163480.1 energy-coupling factor ABC transporter permease -
  ACL0K2_RS08775 (ACL0K2_08775) yacG 1956435..1956632 (-) 198 WP_045163481.1 DNA gyrase inhibitor YacG -
  ACL0K2_RS08780 (ACL0K2_08780) coaE 1956629..1957237 (-) 609 WP_045163482.1 dephospho-CoA kinase -
  ACL0K2_RS08785 (ACL0K2_08785) pilD 1957614..1958480 (-) 867 WP_413044241.1 prepilin peptidase Machinery gene
  ACL0K2_RS08790 (ACL0K2_08790) pilC 1958483..1959700 (-) 1218 WP_413044242.1 type II secretion system F family protein Machinery gene
  ACL0K2_RS08795 (ACL0K2_08795) pilB 1959702..1961405 (-) 1704 WP_413044243.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACL0K2_RS08800 (ACL0K2_08800) pilA/pilAI 1961632..1962051 (+) 420 WP_255983138.1 pilin Machinery gene
  ACL0K2_RS08805 (ACL0K2_08805) pilA/pilAII 1962120..1962545 (+) 426 WP_413044244.1 pilin Regulator
  ACL0K2_RS08810 (ACL0K2_08810) - 1962571..1963962 (+) 1392 WP_413044245.1 O-antigen ligase family protein -
  ACL0K2_RS08815 (ACL0K2_08815) tfpZ 1963971..1964705 (+) 735 WP_413044246.1 TfpX/TfpZ family type IV pilin accessory protein -
  ACL0K2_RS08820 (ACL0K2_08820) - 1964988..1965125 (+) 138 WP_413044247.1 YtoQ family protein -
  ACL0K2_RS08825 (ACL0K2_08825) - 1965110..1965379 (-) 270 Protein_1733 TonB-dependent receptor plug domain-containing protein -
  ACL0K2_RS08830 (ACL0K2_08830) - 1965381..1965716 (+) 336 Protein_1734 YtoQ family protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62013.98 Da        Isoelectric Point: 5.3329

>NTDB_id=1089230 ACL0K2_RS08795 WP_413044243.1 1959702..1961405(-) (pilB) [Pseudomonas sp. YJ42]
MTDNVALSGLAKQMVLTGLLDNKTAQQAQQQAARSKLSLVTYVVQNKLANGRAVAELAAEQFGVAYMDLNALDKESQLKD
VVSEKLARQHRVLPLLKRGHKLFVAVSDPTNQQAVTDIQFSTGLTAEAILVEDDKLGQAIDKFFESSTSGLEDLGDVDLD
GVDVQTVSDDGANDDTIEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAFRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEVELPREALMEEGFP
ADKIGTFKVYGPVGCENCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1089230 ACL0K2_RS08795 WP_413044243.1 1959702..1961405(-) (pilB) [Pseudomonas sp. YJ42]
ATGACCGACAACGTTGCCCTTTCCGGCCTGGCGAAGCAGATGGTGCTAACCGGCCTGCTCGACAACAAGACTGCGCAACA
AGCGCAACAGCAAGCGGCACGCAGCAAGCTGTCGCTGGTGACCTATGTCGTGCAGAACAAACTCGCCAATGGCCGGGCGG
TAGCTGAACTGGCAGCTGAACAGTTTGGTGTCGCATATATGGATCTCAACGCGCTGGATAAGGAAAGCCAGCTAAAAGAC
GTGGTTTCTGAAAAGCTGGCGCGCCAACATCGCGTATTGCCGCTGCTAAAGCGTGGGCACAAGCTGTTCGTGGCTGTATC
GGATCCGACTAACCAGCAGGCTGTAACGGATATTCAGTTCAGTACCGGGCTTACGGCCGAGGCAATCTTGGTCGAGGATG
ACAAGCTTGGGCAGGCCATCGACAAATTTTTCGAGAGCTCGACTTCCGGGCTAGAGGATCTGGGAGATGTAGATCTGGAC
GGCGTTGACGTCCAGACCGTATCGGATGATGGCGCAAACGACGATACGATCGAGGCTGCCGATGATGCGCCCGTCGTGCG
CTTCGTCAACAAGATGCTGCTCGACGCTATTCGAGGCGGCTCATCCGACCTGCACTTCGAGCCTTATGAAAAGGCTTTTC
GGGTACGGCTCCGTACGGATGGCATTCTTCACGAAGTGGCCCGCCCTCCGATTCAGCTGGCGCCGCGTATATCGGCGCGC
TTGAAGGTCATGGCTGGCCTCGATATATCCGAGCGGCGTAAACCGCAGGACGGCCGGATCAAGATGAAGCTGTCCAAGAC
CAAGGCCATCGACTTCCGCGTCAACACATTGCCGACCCTCTGGGGTGAGAAGATAGTCATGCGGATTCTCGATCCGTCTA
GTGCACAAATGGGCATCGATGCGCTGGGTTATGAGGAGGATCAGAAAGAGCTGTATATGAATGCGCTCAAGCAACCACAG
GGCATGATTCTGGTAACCGGGCCGACCGGATCGGGAAAGACCGTTTCGCTTTACACCGGCTTGAATATTCTTAACACGGT
AGACGTGAATATCTCCACGGCGGAAGACCCTGTGGAGATCAACCTTGAGGGGATCAATCAGGTCAACGTCAATCCCCGTC
AGGGCATGGACTTCTCGCAGGCGCTGCGTGCATTTCTGCGCCAGGACCCTGACATCATCATGGTGGGGGAGATCCGGGAC
TTGGAAACCGCCGAAATTGCCATCAAAGCCGCGCAGACCGGGCATATGGTGATGTCGACGCTGCACACCAACAGCGCAGC
GGAAACGCTGACGCGGCTACGCAACATGGGCGTTGCGGCTTTCAATATCGCCACCTCGGTAAACCTGATTATCGCCCAGC
GCCTTGCGCGCAAGCTCTGTAACAGCTGCAAGAAAGAAGTCGAGTTGCCGCGCGAGGCATTAATGGAGGAGGGCTTCCCT
GCCGATAAGATCGGCACCTTCAAAGTTTACGGGCCGGTGGGTTGCGAAAACTGCAAGGGAGGATACAAAGGTCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCTGCCTTGGCCAGGATTATCATGGAGGACGGCAACTCCATCGACATTTCCACCC
AGATGCGCAAAGACGGCTTCAACGATCTGCGAACCTCGGCCTTGGTCAAGGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTCAACCGGGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.259

100

0.593
  pilB Acinetobacter baylyi ADP1

58.511

99.471

0.582
  pilB Legionella pneumophila strain ERS1305867

55.245

100

0.557
  pilB Vibrio parahaemolyticus RIMD 2210633

49.823

99.471

0.496
  pilF Neisseria gonorrhoeae MS11

50.182

97.002

0.487
  pilB Vibrio campbellii strain DS40M4

48.399

99.118

0.48
  pilB Vibrio cholerae strain A1552

50.746

94.533

0.48
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.015

93.122

0.363

Multiple sequence alignment