Detailed information NTG1089228

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACL0K2_RS08785
• Genome accession: NZ_CP178378
• Coordinates: 1957614..1958480 (-)
• Length: 288 a.a.
• NCBI ID: WP_413044241.1
• Uniprot ID: -
• Organism: Pseudomonas sp. YJ42
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1952614..1963480

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACL0K2_RS08755 (ACL0K2_08755)	rpsI	1953322..1953714 (-)	393	WP_021209313.1	30S ribosomal protein S9	-
ACL0K2_RS08760 (ACL0K2_08760)	rplM	1953729..1954157 (-)	429	WP_019342644.1	50S ribosomal protein L13	-
ACL0K2_RS08765 (ACL0K2_08765)	-	1954542..1955579 (+)	1038	WP_045163479.1	NADP(H)-dependent aldo-keto reductase	-
ACL0K2_RS08770 (ACL0K2_08770)	-	1955686..1956378 (+)	693	WP_045163480.1	energy-coupling factor ABC transporter permease	-
ACL0K2_RS08775 (ACL0K2_08775)	yacG	1956435..1956632 (-)	198	WP_045163481.1	DNA gyrase inhibitor YacG	-
ACL0K2_RS08780 (ACL0K2_08780)	coaE	1956629..1957237 (-)	609	WP_045163482.1	dephospho-CoA kinase	-
ACL0K2_RS08785 (ACL0K2_08785)	pilD	1957614..1958480 (-)	867	WP_413044241.1	prepilin peptidase	Machinery gene
ACL0K2_RS08790 (ACL0K2_08790)	pilC	1958483..1959700 (-)	1218	WP_413044242.1	type II secretion system F family protein	Machinery gene
ACL0K2_RS08795 (ACL0K2_08795)	pilB	1959702..1961405 (-)	1704	WP_413044243.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACL0K2_RS08800 (ACL0K2_08800)	pilA/pilAI	1961632..1962051 (+)	420	WP_255983138.1	pilin	Machinery gene
ACL0K2_RS08805 (ACL0K2_08805)	pilA/pilAII	1962120..1962545 (+)	426	WP_413044244.1	pilin	Regulator

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31904.90 Da
• Isoelectric Point: 6.7317

>NTDB_id=1089228 ACL0K2_RS08785 WP_413044241.1 1957614..1958480(-) (pilD) [Pseudomonas sp. YJ42]
MNVVEFLASNPLAFVLCALVLGLLVGSFLNVVIHRLPIMMQRDWRAQAREFLDLPAESVTTFNLILPNSHCPHCDHEIRP
WENIPLVSWLALRGKCSSCRAPISSRYPLIELLCGLLSAYVAWHFGFSWQAGAMLLLTWGLLAMSMIDVDHQLLPDSIVL
PLLWMGLIVNQFGLFATLPDALWGAIAGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSVVGA
VLGTIMLRMQKADSGTAIPFGPYLAIAGWIALLWGDQITSSYLQFARL

Nucleotide

• Length: 867 bp

>NTDB_id=1089228 ACL0K2_RS08785 WP_413044241.1 1957614..1958480(-) (pilD) [Pseudomonas sp. YJ42]
ATGAATGTCGTTGAATTCCTGGCCAGCAACCCGCTGGCCTTTGTTTTATGTGCACTGGTACTCGGCCTATTGGTCGGCAG
TTTCCTCAACGTCGTCATACACCGCCTGCCGATCATGATGCAGCGGGATTGGCGCGCTCAGGCTCGAGAGTTTCTTGATT
TACCTGCCGAGTCAGTCACGACGTTTAATCTGATCCTTCCAAATTCCCATTGCCCCCATTGCGATCACGAGATTCGTCCG
TGGGAGAACATCCCGTTGGTGAGTTGGCTGGCGCTGCGCGGCAAATGCTCCTCGTGTAGGGCACCAATCAGTAGTCGCTA
CCCGCTTATCGAACTGCTCTGTGGGCTGTTGTCCGCCTATGTCGCCTGGCACTTCGGTTTTTCCTGGCAGGCTGGTGCGA
TGTTGCTGTTGACCTGGGGGTTGCTGGCGATGAGCATGATCGACGTCGATCACCAGCTGCTGCCTGATTCCATCGTGCTG
CCGCTGCTTTGGATGGGGTTGATCGTCAATCAGTTCGGGCTGTTCGCTACGTTGCCTGACGCGCTTTGGGGGGCGATTGC
CGGATATCTCAGTCTTTGGTCGGTGTACTGGCTGTTCAAACTGGTGACTGGCAAGGAGGGCATGGGCTACGGCGATTTCA
AGCTACTGGCGATGCTGGGAGCGTGGGGCGGCTGGCAGGTTTTGCCGCTGACCATCCTACTGTCATCTGTAGTGGGCGCA
GTGCTGGGTACCATCATGCTGCGCATGCAAAAGGCGGATAGTGGTACGGCCATTCCTTTTGGGCCCTACCTGGCGATCGC
CGGCTGGATCGCCTTGCTTTGGGGGGATCAGATCACCTCGAGTTATCTGCAGTTCGCCAGGCTCTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

• Type IV leader peptidase family (Peptidase_A24) (136-243)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	56.842	98.958	0.562
pilD	Vibrio campbellii strain DS40M4	53.846	99.306	0.535
pilD	Neisseria gonorrhoeae MS11	52.399	94.097	0.493
pilD	Acinetobacter nosocomialis M2	49.296	98.611	0.486
pilD	Acinetobacter baumannii D1279779	48.944	98.611	0.483