Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACK3BE_RS27020 Genome accession   NZ_CP177237
Coordinates   5906478..5906885 (-) Length   135 a.a.
NCBI ID   WP_010467585.1    Uniprot ID   A0A024EFQ4
Organism   Pseudomonas mandelii strain VR4' HT30-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5901478..5911885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK3BE_RS27005 (ACK3BE_27005) - 5902278..5905232 (+) 2955 WP_077750273.1 DUF748 domain-containing protein -
  ACK3BE_RS27010 (ACK3BE_27010) - 5905232..5905528 (+) 297 WP_282411970.1 DUF2845 domain-containing protein -
  ACK3BE_RS27015 (ACK3BE_27015) - 5905812..5906165 (-) 354 WP_010467586.1 BON domain-containing protein -
  ACK3BE_RS27020 (ACK3BE_27020) pilA 5906478..5906885 (-) 408 WP_010467585.1 pilin Machinery gene
  ACK3BE_RS27025 (ACK3BE_27025) pilB 5907111..5908811 (+) 1701 WP_083375734.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACK3BE_RS27030 (ACK3BE_27030) pilC 5908814..5910031 (+) 1218 WP_010467583.1 type II secretion system F family protein Machinery gene
  ACK3BE_RS27035 (ACK3BE_27035) pilD 5910033..5910905 (+) 873 WP_311096686.1 A24 family peptidase Machinery gene
  ACK3BE_RS27040 (ACK3BE_27040) coaE 5910902..5911525 (+) 624 WP_102593899.1 dephospho-CoA kinase -
  ACK3BE_RS27045 (ACK3BE_27045) yacG 5911522..5911722 (+) 201 WP_077750267.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 13770.94 Da        Isoelectric Point: 8.1079

>NTDB_id=1086337 ACK3BE_RS27020 WP_010467585.1 5906478..5906885(-) (pilA) [Pseudomonas mandelii strain VR4' HT30-3]
MNTQKGFTLIELLIVVAIIGILATFALPAYSKYQARAKVTAGLAEITALKVPFEDLMNSGTNPDLTKVGGTATTSNCTTT
AAGVATDGTGTIGCTILNAPGPVLSKTITLTRSLTGWTCATTVAQEYAPKGCTGV

Nucleotide


Download         Length: 408 bp        

>NTDB_id=1086337 ACK3BE_RS27020 WP_010467585.1 5906478..5906885(-) (pilA) [Pseudomonas mandelii strain VR4' HT30-3]
ATGAATACTCAAAAAGGTTTTACCCTGATCGAACTGCTGATCGTGGTGGCGATCATCGGGATTCTGGCGACGTTTGCGTT
GCCGGCTTATTCGAAGTATCAAGCGCGTGCCAAGGTGACGGCCGGTCTGGCAGAGATCACGGCGTTGAAAGTCCCGTTTG
AGGACCTCATGAACTCTGGTACCAATCCGGATCTGACAAAAGTGGGCGGAACCGCCACGACTTCCAATTGCACAACCACG
GCAGCGGGTGTCGCGACTGATGGTACCGGCACCATCGGTTGCACGATCCTCAACGCTCCAGGTCCGGTCTTGAGCAAAAC
CATTACCCTGACGCGCTCTCTGACTGGCTGGACTTGCGCCACCACAGTGGCTCAGGAGTACGCACCTAAAGGCTGCACAG
GCGTCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A024EFQ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

50

100

0.511
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.445

100

0.481
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.357

100

0.459
  pilA Vibrio cholerae strain A1552

43.357

100

0.459
  pilA Vibrio cholerae C6706

43.357

100

0.459
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.704

100

0.437
  pilA Pseudomonas aeruginosa PAK

39.597

100

0.437
  comP Acinetobacter baylyi ADP1

35.374

100

0.385
  pilA Vibrio parahaemolyticus RIMD 2210633

36.879

100

0.385
  pilA2 Legionella pneumophila str. Paris

37.778

100

0.378
  pilA2 Legionella pneumophila strain ERS1305867

37.037

100

0.37

Multiple sequence alignment