Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACK3BE_RS27025 Genome accession   NZ_CP177237
Coordinates   5907111..5908811 (+) Length   566 a.a.
NCBI ID   WP_083375734.1    Uniprot ID   -
Organism   Pseudomonas mandelii strain VR4' HT30-3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5902111..5913811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK3BE_RS27005 (ACK3BE_27005) - 5902278..5905232 (+) 2955 WP_077750273.1 DUF748 domain-containing protein -
  ACK3BE_RS27010 (ACK3BE_27010) - 5905232..5905528 (+) 297 WP_282411970.1 DUF2845 domain-containing protein -
  ACK3BE_RS27015 (ACK3BE_27015) - 5905812..5906165 (-) 354 WP_010467586.1 BON domain-containing protein -
  ACK3BE_RS27020 (ACK3BE_27020) pilA 5906478..5906885 (-) 408 WP_010467585.1 pilin Machinery gene
  ACK3BE_RS27025 (ACK3BE_27025) pilB 5907111..5908811 (+) 1701 WP_083375734.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACK3BE_RS27030 (ACK3BE_27030) pilC 5908814..5910031 (+) 1218 WP_010467583.1 type II secretion system F family protein Machinery gene
  ACK3BE_RS27035 (ACK3BE_27035) pilD 5910033..5910905 (+) 873 WP_311096686.1 A24 family peptidase Machinery gene
  ACK3BE_RS27040 (ACK3BE_27040) coaE 5910902..5911525 (+) 624 WP_102593899.1 dephospho-CoA kinase -
  ACK3BE_RS27045 (ACK3BE_27045) yacG 5911522..5911722 (+) 201 WP_077750267.1 DNA gyrase inhibitor YacG -
  ACK3BE_RS27050 (ACK3BE_27050) - 5911726..5911941 (-) 216 WP_010467577.1 hypothetical protein -
  ACK3BE_RS27055 (ACK3BE_27055) - 5912005..5912694 (-) 690 WP_083375730.1 energy-coupling factor ABC transporter permease -
  ACK3BE_RS27060 (ACK3BE_27060) - 5912691..5913158 (-) 468 WP_010467572.1 hypothetical protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62652.86 Da        Isoelectric Point: 6.0117

>NTDB_id=1086338 ACK3BE_RS27025 WP_083375734.1 5907111..5908811(+) (pilB) [Pseudomonas mandelii strain VR4' HT30-3]
MNDIALSGLAKQLVMAELLTEQSAQQAYLHAQRSKISLVSYLVQNKLVKSRQVAEIASEHFGMALLDLNCLDKEAQPRGL
VSEKLIRQHHALPLWRRGNKLFVGVSDPTNHQAINDIQFSTGLNTEAILVEDDKLGDAIEKFFDNQATGLEDMADVDLDG
LDIESVDDNKKDAIAGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGMLREVAKPPSQLANRIAARL
KVMASLDISERRKPQDGRIKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKALYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLHNMGIQGFNIATSVTLIIAQRLARKLCSHCKKPIEIPREALLKEGFPE
ERIGSFTIYEPVGCDQCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLIKAMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1086338 ACK3BE_RS27025 WP_083375734.1 5907111..5908811(+) (pilB) [Pseudomonas mandelii strain VR4' HT30-3]
ATGAATGACATCGCCCTAAGCGGTCTGGCCAAGCAACTGGTCATGGCCGAACTGCTCACTGAGCAAAGCGCGCAACAGGC
GTATCTGCATGCCCAACGCAGCAAAATCTCCCTGGTCAGTTACCTGGTGCAGAACAAGCTGGTGAAGAGCCGGCAGGTTG
CCGAGATTGCCTCGGAGCATTTCGGCATGGCGCTGCTCGACCTCAATTGCCTGGACAAGGAAGCCCAGCCTCGAGGGTTG
GTCAGCGAGAAACTGATCCGTCAGCACCACGCCCTGCCCCTCTGGCGCCGTGGCAACAAACTGTTCGTGGGGGTTTCCGA
CCCGACCAATCATCAAGCCATCAATGACATCCAGTTCAGCACCGGTCTGAACACCGAAGCCATTCTGGTAGAGGACGATA
AGCTCGGCGATGCCATCGAGAAGTTCTTCGACAACCAAGCAACCGGCCTGGAAGACATGGCCGATGTTGACCTTGACGGG
CTGGACATCGAGTCGGTCGACGACAACAAAAAGGACGCCATCGCCGGCCAGGATGCCGATGACGCGCCGGTGGTGCGCTT
CGTCCACAAGATGTTGCTCGATGCGATCAAGAGCGGCTCTTCCGACCTGCATTTCGAGCCCTACGAAAAAACCTACCGGG
TGCGGATGCGCACCGACGGCATGCTGCGAGAAGTCGCCAAGCCGCCGAGCCAACTGGCCAACCGCATTGCGGCGCGGCTG
AAAGTCATGGCCAGCCTCGACATCTCCGAGCGGCGCAAACCCCAGGACGGACGGATCAAGATGCGCCTGTCCAAGACCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCATCCAGCG
CACAAATGGGTATCGACGCCCTCGGCTACGAGCCTGAACAGAAAGCCTTGTACATGGCTGCGCTGAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGGCCGACCGGTTCGGGCAAGACCGTGTCGCTCTACACCGGGTTGAATATCCTCAACACCGTGGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAATCAGGTCAACGTCAATCCCAAGCAAG
GGCTGGATTTCGCCCAGGCTCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGTGAGATCCGCGACCTC
GAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACCGGGCACATGGTGCTTTCCACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACCCGCCTGCACAACATGGGCATTCAGGGCTTCAACATCGCCACCTCGGTCACCCTGATCATCGCCCAGCGGC
TGGCGCGCAAACTGTGCAGCCATTGCAAGAAACCCATCGAGATTCCCCGCGAGGCGTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCTCGTTCACGATCTATGAGCCGGTCGGTTGCGATCAGTGCAATGGCGGTTACAAGGGGCGAGTGGGGAT
TTATGAAGTGGTGAAAAACACACCGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCCCTGGAAATCGACATTCAGA
TGCGCAAGGACGGTTTTAATGACTTGCGCACCTCGGGCTTGATCAAGGCCATGCAAGGCGTCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.147

99.47

0.588
  pilB Acinetobacter baumannii D1279779

58.688

99.647

0.585
  pilB Legionella pneumophila strain ERS1305867

55.398

99.823

0.553
  pilB Vibrio cholerae strain A1552

51.15

99.823

0.511
  pilB Vibrio campbellii strain DS40M4

48.772

100

0.491
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

49.198

99.117

0.488
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.13

93.463

0.366

Multiple sequence alignment